Testosterone enhances expression and functional activity of epithelial sodium channel (ENaC), cystic fibrosis transmembrane regulator (CFTR) and sodium hydrogen exchanger (NHE) in vas deferens of sex-steroid deficient male rats

Ramli, Norlisah; Giribabu, Nelli; Salleh, Naguib

doi:10.1016/j.steroids.2018.06.012

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…A study that examined the localization of the ENaCγ (ENaCG) subunit by immunohistochemistry showed staining nearly in the entire row of rat epithelial cells including the cytoplasm (Khadijah Ramli et al, 2018). The authors noted that ENaC is also located on the basolateral membrane.…”

Section: Resultsmentioning

confidence: 99%

“…Our high‐resolution confocal microscopy images showed well preserved and complete images of stereocilia bundles of individual cells (Figures 4, 5, and 9). Previous studies on the histology of the vas deferens using either histochemical staining or electron microscopic ultrastructure showed mostly cut and scattered stereocilia, for example, Francavilla et al (1987) and Khadijah Ramli et al (2018, 2019) that do not represent the dense organization and the structure of the actin bundles.…”

Section: Discussionmentioning

confidence: 96%

“…Cystic fibrosis transmembrane conductance regulator (CFTR) is another ion transporter that is expressed in the vas deferens and modulates the composition of the luminal fluid in the male reproductive tract (Bertog et al, 2000; Carlin et al, 2006; Cheung, Leung, Leung, & Wong, 2003; Khadijah Ramli, Giribabu, & Salleh, 2018; Reynaert et al, 2000). CFTR is a cAMP activated anion channel that secretes mainly Cl¯ ions, and to a lesser extent, HCO 3 ¯ (Csanády, Vergani, & Gadsby, 2019; Saint‐Criq & Gray, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…We undertook the present study, to map the sites of localization of AQP, ENaC, and CFTR in the vas deferens. Recently, the expression of the gamma subunit of ENaC was shown at a low resolution in a partial section of a distal segment of the rat vas deferens by immunohistochemistry (Khadijah Ramli et al, 2018). We aimed to answer the following questions: Is ENaC expressed in both proximal and distal segments of the vas deferens?…”

Section: Introductionmentioning

confidence: 99%

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High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens

Sharma

Kumaran

Hanukoglu

2020

Molecular Reproduction Devel

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Vas deferens is a conduit for sperm and fluid from the epididymis to the urethra. The duct is surrounded by a thick smooth muscle layer. To map the actin cytoskeleton of the duct and its epithelium, we reacted sections of the proximal and distal regions with fluorescent phalloidin. Confocal microscopic imaging showed that the cylinder‐shaped epithelium of the proximal region has a thick apical border of actin filaments that form microvilli. The epithelium of the distal region is covered with tall stereocilia (13–18 µm) that extend from the apical border into the lumen. In both regions, the lateral and basal cell borders showed a thin lining of actin cytoskeleton. The vas deferens epithelium contains various channels to regulate the fluid composition in the lumen. We mapped the localization of the epithelial sodium channel (ENaC), aquaporin‐9 (AQP9), and cystic fibrosis transmembrane conductance regulator (CFTR) in the rat and mouse vas deferens. ENaC and AQP9 immunofluorescence were localized on the luminal surface and stereocilia and also in the basal and smooth muscle layers. CFTR immunofluorescence appeared only on the luminal surface and in smooth muscle layers. The localization of all three channels on the apical surface of the columnar epithelial cells provides clear evidence that these channels are involved concurrently in the regulation of fluid and electrolyte balance in the lumen of the vas deferens. ENaC allows the flow of Na+ ions from the lumen into the cytoplasm, and the osmotic gradient generated provides the driving force for the passive flow of water through AQP channels.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 96%