2017
DOI: 10.1016/j.mce.2017.02.005
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Testosterone promotes tube formation of endothelial cells isolated from veins via activation of Smad1 protein

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Cited by 10 publications
(5 citation statements)
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“…Nevertheless, our results suggest that, apart from these proangiogenic factors, steroid hormones are also involved in tube formation, as previously observed by other authors [72]. It has been shown that the addition of androgens increases the formation of vascular-like structures [73]. Our results support this hypothesis, since the concentrations of DHEA and DHT in the culture medium decreased following the addition of VEGF to neoplastic cells, which suggests that the cells consume these androgens to increase the formation of tubular-like structures.…”
Section: Discussionsupporting
confidence: 90%
“…Nevertheless, our results suggest that, apart from these proangiogenic factors, steroid hormones are also involved in tube formation, as previously observed by other authors [72]. It has been shown that the addition of androgens increases the formation of vascular-like structures [73]. Our results support this hypothesis, since the concentrations of DHEA and DHT in the culture medium decreased following the addition of VEGF to neoplastic cells, which suggests that the cells consume these androgens to increase the formation of tubular-like structures.…”
Section: Discussionsupporting
confidence: 90%
“…Caveolae/Rafts isolation kit (#CS0750) was purchased from Sigma and was used according to manul of the kit and protocols reported by our group and others 59 , 60 . Cells were homogenized in 1 mL of lysis buffer (10 mM Tris-HCl, pH=7.4, 1 mM EDTA, 200 mM sucrose and proteases inhibitors mix).…”
Section: Methodsmentioning
confidence: 99%
“…To investigate the effect of PPD formulations on angiogenesis in vitro, HUVECs were seeded on Matrigel (BD Biosciences, USA), which was used to precoat a 96-well plate. The samples were incubated with the PPD formulations for 8 h. Tube networks were quantified by quantifying the number of nodes under an inverted microscope (Olympus CKX41, Tokyo, Japan) [ 25 ].…”
Section: Methodsmentioning
confidence: 99%