Tests for coxsackie B virus-specific IgM

Pattison, J. R.

doi:10.1017/s0022172400028965

Cited by 19 publications

(6 citation statements)

References 9 publications

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“…We report the detection of the enterovirus-specific IgG class and subclasses by using purified CB3 virus as the enterovirus antigen. In several studies, immunoassays involving the use of enterovirus antigen for coating the solid phase have given group-specific (broadly reactive) rather than type-specific results, even when the antigen consists of purified virions (5,10,12,14,19). This was also the case in the present study.…”

Section: Discussionsupporting

confidence: 59%

Subclass restriction of human enterovirus antibodies

Torfason¹,

Reimer²,

Keyserling³

1987

J Clin Microbiol

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We studied antibodies to enteroviruses in four groups of serum specimens: those from healthy adults, cord blood specimens, serum specimens known to contain immunoglobulin M (IgM) to coxsackie B (CB) viruses by radioimmunoassay, and serum specimens from children with symptomatic enteroviral infections. Enzymelinked immunosorbent assays (ELISAs) were developed to detect the IgG classand subclass (IgGl, IgG2, IgG3, and IgG4)-specific responses to CB3. The CB3 virus ELISA was not type specific. There was very poor correlation between CB3 virus neutralizing titer and IgG anti-CB3 virus ELISA results, indicating that antibodies to heterologous picornaviruses cross-react with CB3 virus in the assay. All serum specimens tested except one were IgG positive for CB3 virus. All 32 cord serum specimens were positive for IgGl and IgG3. No enterovirus-specific IgG2 or IgG4 was detected in any serum specimen tested. Most serum specimens from the IgM-positive group, healthy adults, and children with enterovirus infections were positive for IgGl and IgG3. Class and subclass antibody titers remained constant over time. IgG antibodies to enteroviruses appear to be restricted to the IgGl and IgG3 subclasses. This pattern is similar to results obtained by other investigators evaluating IgG subclass antibodies to protein antigens.

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Section: Discussionsupporting

confidence: 59%

Subclass restriction of human enterovirus antibodies

Torfason¹,

Reimer²,

Keyserling³

1987

J Clin Microbiol

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“…One would involve removing the enterovirus and rhinovirus specific epitopes from VP1 antigens and another one could involve pre-incubating serum samples with said epitopes to reduce binding to the plated antigens. Using this panel of antigens for studying the IgM fraction of sera could also make the test more specific and relevant for diagnosing acute infections, as IgM responses have been shown to be less cross-reactive than IgG-response [ 39 ]. Studying only the IgM antibodies would however require a serum fractionation step prior to applying the samples to the assay if it is to stay on this platform.…”

Section: Discussionmentioning

confidence: 99%

Multiplexed High-Throughput Serological Assay for Human Enteroviruses

et al. 2020

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Immunological assays detecting antibodies against enteroviruses typically use a single enterovirus serotype as antigen. This limits the ability of such assays to detect antibodies against different enterovirus types and to detect possible type-specific variation in antibody responses. We set out to develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types encompassing all human infecting species. Seven recombinant VP1 proteins from enteroviruses EV-A to EV-D and rhinoviruses RV-A to RV-C species were produced. Using Meso Scale Diagnostics U-PLEX platform we were able to study antibody reactions against these proteins as well as non-structural enterovirus proteins in a single well with 140 human serum samples. Adults had on average 33-fold stronger antibody responses to these antigens (p < 10−11) compared to children, but children had less cross-reactivity between different enterovirus types. The results suggest that this new high-throughput assay offers clear benefits in the evaluation of humoral enterovirus immunity in children, giving more exact information than assays that are based on a single enterovirus type as antigen.

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“…Alternatively, non-labeled virus and a labeled antibody with high affinity for the virus that is not reactive with cellular or serum antigens present in the assay can be used [El-Hagrassy et al, 19801. For an overview of assays and their specificities, see the review by Pattison [1983].…”

Section: Introductionmentioning

confidence: 99%

Comparison of five elsa assays for IgG antibody against coxsackievirus B1

Torfason

Galindo

Keyserling

1988

Journal of Medical Virology

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Enterovirus type and group specificities of five different IgG ELISA methods were compared, using neutralization titration tests as an indicator of the presence or absence of antibodies to coxsackie B (CB) viruses. One of the ELISA assays was a "standard" IgG assay, where the solid phase was coated directly with the purified virus, followed by incubations with human serum, biotinylated anti-human-IgG, streptavidin-peroxidase, and the substrate/chromogen. In a modified standard assay, blocking of common epitopes was attempted by incubating the CB1 virus antigen on the solid phase with a rabbit antiserum to CB5 before the human serum was added. In another modification the serum dilution buffer contained heat-denatured heterologous enteroviruses in an attempt to consume human antibodies reacting with common epitopes. In one assay the purified CB1 virus was captured by purified horse anti-CB1 IgG on the solid phase, before incubation with human serum. In the last of the five assays the serum specimen was incubated with CB1 virus (in the liquid phase) before the virus or virus-antibody complex was captured with purified horse anti-CB1-IgG. Reactions against common antigens dominated in the first three assays. The antigen-capture assay appeared to be at least predominantly type specific. Our data indicate that the liquid-phase assay may be type specific, but more studies are needed. The method of virus purification was critical for the type specificity of the antigen-capture and liquid-phase assays.

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Tests for coxsackie B virus-specific IgM

Cited by 19 publications

References 9 publications

Subclass restriction of human enterovirus antibodies

Subclass restriction of human enterovirus antibodies

Multiplexed High-Throughput Serological Assay for Human Enteroviruses

Comparison of five elsa assays for IgG antibody against coxsackievirus B1

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