Tethered-bilayer lipid membranes as a support for membrane-active peptides

Cornell, Bruce; Krishna, Gowri; Osman, Péter; Pace, Ronald; Wieczorek, Lech

doi:10.1042/bst0290613

Cited by 72 publications

(48 citation statements)

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“…An immunosensing device, comprising a lipid membrane incorporating ion channels tethered to the surface of a gold electrode, has been reported [39]. This article describes key steps in the assembly of the device and provides further evidence for its proposed sensing mechanism.…”

Section: Applications Of Nano Biosensors Based On Lipid Films For Usementioning

confidence: 99%

Lipid Based Nano‐biosensors for Medical Diagnostics

Nikoleli

Nikolelis

Tzamtzis

2012

Intelligent Nanomaterials

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Lipid membrane based nano-biosensors have been exploited in biosensor technology for the last 30 years. Recent advances in the stabilization of lipid membranes have recently increased the number of publications; however, the applications for the construction of medical devices for diagnostics are still limited. The objective of the present article is to describe recent methods for the construction of stabilized lipid film biosensors and advances in uses of lipid film based nano-biosensors for medical diagnostics. These novel devices could be used for the quantification of high-risk toxicants in the human body. Such developments include the production of biochips to detect neurotransmitters, proteins, DNA, and small molecules, such as glucose. Recent research is focused on the construction of a portable device that will be commercialized and readily be used by nonskilled personnel for the remote sensing of biomedical interest analytes.

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Section: Applications Of Nano Biosensors Based On Lipid Films For Usementioning

confidence: 99%

Lipid Based Nano‐biosensors for Medical Diagnostics

Nikoleli

Nikolelis

Tzamtzis

2012

Intelligent Nanomaterials

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“…This has been solved to some extent by the deployment of supported [17] and suspended [18] bilayer and monolayer/bilayer [19] devices as putative sensor systems. Nonetheless, these developments have become unnecessarily complicated to use as simple detection systems in spite of their biological relevance [20,21]. In fact their complexity is related to their configuration and the mechanisms involved in the sensing which involve some kind of binding system [20,21].…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, these developments have become unnecessarily complicated to use as simple detection systems in spite of their biological relevance [20,21]. In fact their complexity is related to their configuration and the mechanisms involved in the sensing which involve some kind of binding system [20,21]. The phospholipid on Hg system occupies a unique niche among membrane-based sensors and has received considerable scientific interest [22].…”

Section: Introductionmentioning

confidence: 99%

High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes

et al. 2011

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A sensing system for the rapid on-line screening of lipids and biomembrane active compounds in water samples has been successfully developed. The sensor consists of a flow cell and incorporated wafer-based device with on-chip mercury on platinum (Pt/Hg) working electrode and platinum (Pt) auxiliary and pseudo-reference electrodes. To optimise system performance, the following experiments were carried out: (i) Deposition and removal of phospholipid layers on and from Pt/Hg electrodes respectively, (ii) Effect of electrode size on signal, (iii) Monitoring of different phospholipids deposited in flow cell and, (iv) Detection of phospholipid monolayer interaction with representative compounds. The results showed that: (i) Miniaturisation and ruggedisation of the mercury (Hg)/phospholipid system has been successfully achieved, (ii) Rapid cyclic voltammetry facilitates repetitive dioleoyl phosphatidylcholine (DOPC) monolayer formation on Hg from aqueous DOPC dispersion and, (iii) The device responds selectively to organic compounds injected into electrolyte flow.

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“…system could then be incorporated directly onto a conducting polymer/glassy carbon support, or some other immobilizing substrate surface (e.g, ref. [36], Fig. 8).…”

Section: Towards the Design Of Biomimetic Catalystsmentioning

confidence: 99%

The Biomimetic Inspiration for Renewable Hydrogen Fuel Production from Water Oxidation within Artificial Photosynthesis

Pace

Stranger

2012

Aust. J. Chem.

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The thermodynamic constraints for the operation of the water oxidizing Mn 4 /Ca cluster within Photosystem II (PS II) are discussed. These are then examined in the light of the known redox chemistry of hydrated Mn-oxo systems and relevant model compounds. It is shown that the latest high resolution crystal structure of cyanobacterial PS II suggests an organization of the mono Ca tetranuclear Mn cluster that naturally accommodates the stringent requirements for successive redox potential constancy, with increasing total oxidation state, which the enzyme function imposes. This involves one region of the Mn 4 /Ca cluster being dominantly involved with substrate water binding, while a separate, single Mn is principally responsible for the redox accumulation function. Recent high level computational chemical investigations by the authors' strongly support this, with a computed pattern of Mn oxidation states throughout the catalytic cycle being completely consistent with this interpretation. Strategies to design synthetic, biomimetic constructs utilizing this approach for efficient electrolytic generation of hydrogen fuel within artificial photosynthesis are briefly discussed.

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Tethered-bilayer lipid membranes as a support for membrane-active peptides

Cited by 72 publications

References 0 publications

Lipid Based Nano‐biosensors for Medical Diagnostics

Lipid Based Nano‐biosensors for Medical Diagnostics

High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes

The Biomimetic Inspiration for Renewable Hydrogen Fuel Production from Water Oxidation within Artificial Photosynthesis

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