Tetra-, penta-, and hexa-nor-lanostane triterpenes from the medicinal fungus Ganoderma australe

Zhou, Lin; Akbar, Subiy; Wang, Mengxi; Chen, He‐Ping; Liu, Ji‐Kai

doi:10.1007/s13659-022-00356-x

Cited by 5 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Triterpenoids are one of the most studied compounds contained in Garnoderma spp. Recently, many new lanostane-type triterpenoids have been identified in G. australe extracts from mycelia 44 and fruiting bodies 45 – 47 using alcohol and ethyl acetate. Some of these compounds have demonstrated antituberculosis activity, moderate inhibition of nitric oxide production and a significant inhibitory effect on the activity of α-glucosidase.…”

Section: Discussionmentioning

confidence: 99%

In vitro cholesterol lowering activity of Ganoderma australe mycelia based on mass spectrometry, synchrotron Fourier-transform infrared analysis and liver-spheroid bioactivity

Wongkhieo,

Tangmesupphaisan,

Siriwaseree

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Mycelia were cultivated from a Thai wild mushroom identified as Ganoderma australe based on polymerase chain reaction (PCR) and morphological analyses. The mycelial extracts were examined for their active ingredients using a liquid chromatography-tandem mass spectrometry (LC‒MS/MS) method. This revealed the presence of lovastatin and tentative compounds including p-coumaric, nicotinamide, gamma-aminobutyric acid, choline, nucleosides, amino acids, and saccharides. The extracts had an inhibitory effect on the activity of HMG-CoA reductase in a concentration-dependent manner. At 2.5 mg/mL, the G. australe extracts did not interfere with the viability of HepG2 spheroids, but their biochemical composition was altered as determined by Fourier-transform infrared (FTIR) spectroscopy. The lipid profile of the spheroids treated with the mycelial extract was distinct from that of the control and the 5 µM lovastatin treatment, corresponding with the production of cholesterol by the spheroids. The mycelia of G. australe increased the percentage of high-density lipoprotein (HDL) production to 71.35 ± 2.74%, compared to the control and lovastatin-treated spheroids (33.26 ± 3.15% and 32.13 ± 3.24%, respectively). This study revealed the superior effect of natural compound mixtures to pure lovastatin, and the potential use of Thailand’s wild G. australe as a functional food to prevent or alleviate hypercholesterolemia.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro cholesterol lowering activity of Ganoderma australe mycelia based on mass spectrometry, synchrotron Fourier-transform infrared analysis and liver-spheroid bioactivity

Wongkhieo,

Tangmesupphaisan,

Siriwaseree

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Basidiocarps, mycelia, and spores of Ganoderma are known as the source of biologically active lanostane-type triterpenoids [17][18][19]60,[73][74][75]. These compounds are structurally diverse and include acids and alcohols.…”

Section: Triterpenoids In Surface Layer Of Ganoderma Basidiocarpsmentioning

confidence: 99%

“…The presence and distribution of target bioactive components in the fruiting bodies (basidiocarps) and other parts of this fungus may depend on the genetic background, cultivation conditions, and developmental stage. There are many studies of cell wall macromolecules and secondary metabolites in Ganoderma basidiocarps based on various analytical methods, including photometric assays [10], different techniques of mass spectrometry (MS) [11][12][13][14], one-and two-dimensional (1D, 2D) nuclear magnetic resonance (NMR) spectroscopy [15][16][17][18][19], gas chromatography with MS detection (GC-MS) [20], high-performance liquid chromatography (HPLC) [10,21], and HPLC with MS [21][22][23][24] or NMR [25] detection. These methods exhibit high accuracy, selectivity, and sensitivity to the structure of the analytes.…”

Section: Introductionmentioning

confidence: 99%

Mid-Infrared Spectroscopic Study of Cultivating Medicinal Fungi Ganoderma: Composition, Development, and Strain Variability of Basidiocarps

Synytsya,

Bleha,

Skrynnikova

et al. 2023

JoF

View full text Add to dashboard Cite

Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy was proposed for rapid, versatile, and non-invasive screening of Ganoderma basidiocarps to assess their potential for specific applications. Fifteen species and strains of this fungus were selected for analysis, and fine sections at different parts of young and mature basidiocarps were obtained. The spectra of fungal samples showed significant differences interpreted in terms of biochemical composition using characteristic bands of proteins, polysaccharides, lipids, and triterpenoids. Obviously, for the transverse sections in trama, especially in the basal part, the most intense bands at 950–1200 cm−1 corresponded to polysaccharide vibrations, while for the superficial sections, the bands of carbonyl and aliphatic groups of triterpenoids at 1310–1470, 1550–1740, and 2850–2980 cm−1 predominated. The pilei, especially hymenium tubes, apparently contained more proteins than the bases and stipes, as evidenced by the intense bands of amide vibrations at 1648 and 1545–1550 cm−1. The specificity of the Ganoderma basidiocarp is a densely pigmented surface layer rich in triterpenoids, as proved by ATR-FTIR spectroscopy. The spectral differences corresponding to the specificity of the triterpenoid composition may indicate the prospects of individual strains and species of this genus for cultivation and further use in food, cosmetics, or medicine.

show abstract

Three previously undescribed metabolites from Cordyceps cicadae JXCH-1, an entomopathogenic fungus

Fan,

Liu,

Zhao

et al. 2023

Nat. Prod. Bioprospect.

View full text Add to dashboard Cite

Three previously undescribed compounds, cordycicadione (1), cordycicadin F (2), and 7-hydroxybassiatin (3), were isolated from the cultures of Cordyceps cicadae JXCH1, an entomopathogenic fungus. Their structures and relative configurations were elucidated primarily by NMR spectroscopic analysis. The absolute configurations of 1 and 2 were determined by ECD calculations. Single-crystal X-ray diffraction method was adopted to determine the absolute configuration of 3. Compound 2 is a polycyclic polyketide with an unusual enol ether moiety and a spiro ring. The compounds obtained in this study were subjected to screening their inhibition against the proliferation of the human lung cancer cell line A549 and the production of nitric oxide in murine macrophages RAW264.7. Graphical Abstract

show abstract

Tetra-, penta-, and hexa-nor-lanostane triterpenes from the medicinal fungus Ganoderma australe

Cited by 5 publications

References 22 publications

In vitro cholesterol lowering activity of Ganoderma australe mycelia based on mass spectrometry, synchrotron Fourier-transform infrared analysis and liver-spheroid bioactivity

In vitro cholesterol lowering activity of Ganoderma australe mycelia based on mass spectrometry, synchrotron Fourier-transform infrared analysis and liver-spheroid bioactivity

Mid-Infrared Spectroscopic Study of Cultivating Medicinal Fungi Ganoderma: Composition, Development, and Strain Variability of Basidiocarps

Three previously undescribed metabolites from Cordyceps cicadae JXCH-1, an entomopathogenic fungus

Contact Info

Product

Resources

About