Tetracycline Resistance Genes in Activated Sludge Wastewater Treatment Plants

Auerbach, Eric; Seyfried, Erin E.; McMahon, Katherine D.

doi:10.2175/193864706783749495

Cited by 49 publications

(83 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent concerns with land application of biosolids have focused on the potential for enhanced levels of antibiotic resistant bacteria and the spread of antibiotic resistance genes (Auerbach et al, 2007;Brooks et al, 2007;Silbergeld et al, 2008). Data from this present study show that the high biosolid-treated soils had higher antibiotic resistant bacteria (ARB) concentrations than the unamended soils for ampicillin, cephalothin, and ciprofl oxacin but decreased concentrations for tetracycline.…”

Section: Discussion and Summarymentioning

confidence: 61%

Long‐Term Effects of Land Application of Class B Biosolids on the Soil Microbial Populations, Pathogens, and Activity

et al. 2010

View full text Add to dashboard Cite

This study evaluated the influence of 20 annual land applications of Class B biosolids on the soil microbial community. The potential benefits and hazards of land application were evaluated by analysis of surface soil samples collected following the 20th land application of biosolids. The study was initiated in 1986 at the University of Arizona Marana Agricultural Center, 21 miles north of Tucson, AZ. The final application of biosolids was in March 2005, followed by growth of cotton (Gossypium hirsutum L.) from April through November 2005. Surface soil samples (0-30 cm) were collected monthly from March 2005, 2 wk after the final biosolids application, through December 2005, and analyzed for soil microbial numbers. December samples were analyzed for additional soil microbial properties. Data show that land application of Class B biosolids had no significant long-term effect on indigenous soil microbial numbers including bacteria, actinomycetes, and fungi compared to unamended control plots. Importantly, no bacterial or viral pathogens were detected in soil samples collected from biosolid amended plots in December (10 mo after the last land application) demonstrating that pathogens introduced via Class B biosolids only survived in soil transiently. However, plots that received biosolids had significantly higher microbial activity or potential for microbial transformations, including nitrification, sulfur oxidation, and dehydrogenase activity, than control plots and plots receiving inorganic fertilizers. Overall, the 20 annual land applications showed no long-term adverse effects, and therefore, this study documents that land application of biosolids at this particular site was sustainable throughout the 20-yr period, with respect to soil microbial properties.

show abstract

Section: Discussion and Summarymentioning

confidence: 61%

Long‐Term Effects of Land Application of Class B Biosolids on the Soil Microbial Populations, Pathogens, and Activity

et al. 2010

View full text Add to dashboard Cite

show abstract

“…McKinney and Pruden (2012) investigated the damage to ARGs by UV irradiation and found that the UV dose between 200 and 400 mJ cm À2 results in 3-4 log reduction in ARGs. Nevertheless, UV light did not contribute to the elimination of tetR, tetG and tetQ genes (Auerbach et al 2007).…”

Section: Introductionmentioning

confidence: 88%

Effects of ultraviolet disinfection on antibiotic-resistantEscherichia colifrom wastewater: inactivation, antibiotic resistance profiles and antibiotic resistance genes

Zhang

Wang

et al. 2017

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Resistance in aquatic environments can be intrinsic, but it can also be the result of anthropogenic influences. Run-off from agricultural areas (Chee-Sanford et al 2001) and wastewater treatment facilities (Iwane et al 2001;Auerbach et al 2007) are also sources of antibioticresistant bacteria (ARB) and ARGs in aquatic environments.…”

Section: Introductionmentioning

confidence: 99%

Characterization of tetracycline-resistant bacteria in an urbanizing subtropical watershed

2013

View full text Add to dashboard Cite

Aims: The objective of this study was to determine whether varying levels of urbanization influence the dominant bacterial species of mildly resistant (0Á03 mmol l À1 tetracycline) and highly resistant (0Á06 mmol l À1 tetracycline) bacteria in sediment and water. Also, the level of urbanization was further evaluated to determine whether the diversity of tetracycline resistance genes present in the isolates and the capability of transferring their resistance were influenced. Methods and Results: Sediment and water samples collected from five sampling sites were plated in triplicate on nutrient agar plates with a mild dose (0Á03 mmol l À1 ) and a high dose (0Á06 mmol l À1 ) of tetracycline. Five colonies from each plate plus an additional five from each triplicate group were randomly selected and isolated on nutrient agar containing 0Á03 mmol l À1 tetracycline (400 isolates). The isolates were identified by 16S rRNA gene sequencing and comparison to GenBank using BLAST. The isolates were also screened for 15 tetracycline resistance genes using a multiplex PCR assay and their ability to transfer resistance through conjugation experiments using a kanamycin-resistant Escherichia. coli K-12 strain labelled with a green fluorescent protein gene. Results from this study indicate that the dominant resistant organisms in this watershed are Acinetobacter spp., Chryseobacterium spp., Serratia spp., Pseudomonas spp., Aeromonas spp. and E. coli. All of these organisms are Gram negative and are closely related to pathogenic species. A majority of the isolates (66%) were capable of transferring their resistance, and there was a greater incidence of tet resistance transfer with increasing urbanization. Also, it was determined that the dominant resistance genes in the watershed are tet(W) and tet(A). Conclusion: Urbanization significantly affected dominant tetracycline-resistant bacteria species, but did not affect dominant resistance genes. There was correlation between increased urbanization with an increase in the ability to transfer tetracycline resistance. This indicates that urban areas may select for bacterial species that are capable of transferring resistance. Significance and Impact of Study: These results indicate that urbanization influences the occurrence of tetracycline-resistant bacteria and the potential for transfer of resistance genes.

show abstract

Tetracycline Resistance Genes in Activated Sludge Wastewater Treatment Plants

Cited by 49 publications

References 0 publications

Long‐Term Effects of Land Application of Class B Biosolids on the Soil Microbial Populations, Pathogens, and Activity

Long‐Term Effects of Land Application of Class B Biosolids on the Soil Microbial Populations, Pathogens, and Activity

Effects of ultraviolet disinfection on antibiotic-resistantEscherichia colifrom wastewater: inactivation, antibiotic resistance profiles and antibiotic resistance genes

Characterization of tetracycline-resistant bacteria in an urbanizing subtropical watershed

Contact Info

Product

Resources

About