Tetrameric peptide purified from hydrolysates of biodiesel byproducts of Nannochloropsis oculata induces osteoblastic differentiation through MAPK and Smad pathway on MG-63 and D1 cells

Nguyen, M. Hong; Qian, Zhong‐Ji; Nguyen, Van-Tinh; Choi, Il‐Whan; Heo, Soo‐Jin; Oh, Chul; Kang, Do‐Hyung; Kim, GeunHyung; Jung, Won‐Kyo

doi:10.1016/j.procbio.2013.06.031

Cited by 39 publications

(24 citation statements)

References 32 publications

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“…Biselyngbyaside, a macrolide compound from marine cyanobacteria Lyngbya sp., also inhibits osteoclastogenesis and induces apoptosis in mature osteoclasts [ 31 ]. Similar results are presented for a range of extracts, such as microalgae byproducts [ 34 ] and a depsipeptide (largazole) sourced from cyanobacteria [ 92 ]. Another interesting prokaryote is Alteromonas infernus , which is a deep sea species known to produce a high molecular weight polysaccharide.…”

Section: Assorted Taxasupporting

confidence: 68%

Bioactive Compounds from Marine Organisms: Potential for Bone Growth and Healing

Carson

Clarke

2018

Marine Drugs

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Marine organisms represent a highly diverse reserve of bioactives which could aid in the treatment of a wide range of diseases, including various musculoskeletal conditions. Osteoporosis in particular would benefit from a novel and effective marine-based treatment, due to its large disease burden and the inefficiencies of current treatment options. Osteogenic bioactives have been isolated from many marine organisms, including nacre powder derived from molluscan shells and fucoidan—the sulphated polysaccharide commonly sourced from brown macroalgae. Such extracts and compounds are known to have a range of osteogenic effects, including stimulation of osteoblast activity and mineralisation, as well as suppression of osteoclast resorption. This review describes currently known soluble osteogenic extracts and compounds from marine invertebrates and algae, and assesses their preclinical potential.

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Section: Assorted Taxasupporting

confidence: 68%

Bioactive Compounds from Marine Organisms: Potential for Bone Growth and Healing

Carson

Clarke

2018

Marine Drugs

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“…46,47 MAPKs have been implicated in the pathways relevant to cell proliferation, apoptosis, as well as differentiation. 11,48 The activation of MAPK signalling cascade contributes to the induction of expression of the phenotypic markers of osteoblastic differentiation. 11 Additionally, the Smad pathway is also affected during osteoblast differentiation through phosphorylation of regulatory Smads.…”

Section: Discussionmentioning

confidence: 99%

“…11,48 The activation of MAPK signalling cascade contributes to the induction of expression of the phenotypic markers of osteoblastic differentiation. 11 Additionally, the Smad pathway is also affected during osteoblast differentiation through phosphorylation of regulatory Smads. 49 We had shown that FBP-KSA induced phosphorylation of MAPKs (p38, ERK, and JNK) and Smads.…”

Section: Discussionmentioning

confidence: 99%

“…Reduced bone density and strength with increased risk of spontaneous and traumatic bone fracture are hallmarks of osteoporosis, the most common human metabolic bone disorder . According to the World Health Organization, postmenopausal osteoporosis afflicts nearly 30% of women older than 65 years of age . Although the drugs that are used conventionally for the treatment of osteoporosis inhibit bone resorption, they may also suppress bone formation and contribute to the pathogenesis of osteonecrosis .…”

Section: Introductionmentioning

confidence: 99%

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Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

Heo

Nam

et al. 2018

Cell Biochemistry & Function

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Fish bone, a by-product of fishery processing, is composed of protein, calcium, and other minerals. The objective of this study was to investigate the effects of a bioactive peptide isolated from the bone of the marine fish, Johnius belengerii, on the osteoblastic differentiation of MC3T3-E1 pre-osteoblasts. Post consecutive purification by liquid chromatography, a potent osteogenic peptide, composed of 3 amino acids, Lys-Ser-Ala (KSA, MW: 304.17 Da), was identified. The purified peptide promoted cell proliferation, alkaline phosphatase activity, mineral deposition, and expression levels of phenotypic markers of osteoblastic differentiation in MC3T3-E1 pre-osteoblast. The purified peptide induced phosphorylation of mitogen-activated protein kinases, including p38 mitogen-activated protein kinase, extracellular regulated kinase, and c-Jun N-terminal kinase as well as Smads. As attested by molecular modelling study, the purified peptide interacted with the core interface residues in bone morphogenetic protein receptors with high affinity. Thus, the purified peptide could serve as a potential pharmacological substance for controlling bone metabolism.

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“…A number of marine‐derived protein hydrolysates or peptides have been reported to show bone health‐promoting effects. Tetrapeptide MPDW obtained from marine microalgae, Nannochloropsis oculata , by alcalase hydrolysis, induced osteoblast differentiation through mitogen‐activated protein kinases and Smad signaling pathway in MG‐63 and mouse MSCs (Nguyen et al, ). Protein hydrolysates derived from blue mussel and ark shell by pepsin hydrolysis increased osteoblast differentiation in mouse MSCs by activating the BMP signaling pathway followed by activation of transcription factors (Hyung, Ahn, & Je, ).…”

Section: Food‐derived Bioactive Peptides: Production and Bone Health mentioning

confidence: 99%

Bone health-promoting bioactive peptides

Ahn

2018

J Food Biochem

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Bioactive peptides, derivatives of proteins, show versatile biological effects and represent potential health-promoting agents as functional food ingredients and/or nutraceuticals. Bone health depends on the balance between bone formation and resorption. When the balance is disrupted, bone diseases such as osteoporosis and fragility fractures may result. Accumulating evidence suggests that peptides derived from endogenous proteins and food proteins enhance bone health.This article reviews the literature on peptides exhibiting bone health-promoting effects. Possible biochemical mechanisms and production of these peptides are briefly discussed.

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Tetrameric peptide purified from hydrolysates of biodiesel byproducts of Nannochloropsis oculata induces osteoblastic differentiation through MAPK and Smad pathway on MG-63 and D1 cells

Cited by 39 publications

References 32 publications

Bioactive Compounds from Marine Organisms: Potential for Bone Growth and Healing

Bioactive Compounds from Marine Organisms: Potential for Bone Growth and Healing

Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

Bone health-promoting bioactive peptides

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