Tetranuclear and binuclear iron-sulfur clusters in succinate dehydrogenase: A method of iron quantitation by formation of paramagnetic complexes

Salerno, John C.; Ohnishi, Tomo̧ko; Lim, Joyce; King, Tsoo E.

doi:10.1016/0006-291x(76)90884-6

Cited by 52 publications

(18 citation statements)

References 12 publications

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“…However, we interpret this spectrum as showing features due to an additional reaction with excess NO. It is known that iron-sulphur proteins treated with NO gas can give an EPR spectrum characteristic of an ironcysteine-NO complex [8,9]. A spectrum of this complex is shown in fig.2(c).…”

Section: Resultsmentioning

confidence: 99%

Stability of the nitrosyl—sirohaem complex of plant nitrite reductase, investigated by EPR spectroscopy

et al. 1980

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Section: Resultsmentioning

confidence: 99%

Stability of the nitrosyl—sirohaem complex of plant nitrite reductase, investigated by EPR spectroscopy

et al. 1980

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“…Va sorelaxation during the very early phase of ischemia (Morikawa et aI., 1992), the inhibition of platelet and leukocyte adhesion (Radomski et aI., 1987;Kubes et aI., 1991), and the blockade of NMDA receptors (Lei et aI., 1992;Manzoni et aI., 1992) may protect the brain from ischemic injury. The cytotoxicity of NO through the formation of perox ynitrites (Beckman, 1991), the mediation of gluta mate neurotoxicity (Dawson et aI., 1991), and the inhibition of several enzymes containing a nonheme iron-sulfur complex (Salerno and Ohnishi, 1976;Dawson et aI. , 1992) may exacerbate injury.…”

Section: Discussionmentioning

confidence: 99%

Electron Paramagnetic Resonance (EPR) Detection of Nitric Oxide Produced during Forebrain Ischemia of the Rat

Tominaga¹,

Sato

Ohnishi

et al. 1994

J Cereb Blood Flow Metab

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Summary: To detect if nitric oxide (NO) is produced in rat forebrain ischemia, we applied an electron paramag netic resonance (EPR) NO-trapping technique. We also performed a detailed characterization of the technique. Diethyldithiocarbamate (DETC) and Fe-citrate were used as NO-trapping reagents. Under controlled ventilation, forebrain ischemia was produced by occlusion of both carotid arteries combined with hemorrhagic hypotension at 50 mm Hg for 15 min. DETC and Fe were administered 30 min prior to the onset of ischemia. During ischemia, the cerebral cortex was removed, and EPR samples were prepared. At liquid nitrogen temperatures, the NO-Fe DETC signal (a triplet signal centered at g = 2.039 with the hyperfine coupling constant aN of 13 G) was detected Nitric oxide (NO) is a highly reactive regulatory molecule with a wide range of distribution through out the body, including the brain. Based upon its physiological role, the major sources of NO produc tion in the brain are thought to be endothelial cells and neurons (Palmer et al., 1988;Moncada et al., 1991;Bredt and Snyder, 1992). NO or a related compound released from endothelial cells serves as an endothelium-derived relaxing factor that main tains and regulates vascular tone (Moncada et al., 1991; Vanin, 1991;Prado et al., 1992 Abbreviations used: CCA, common carotid artery; DEA/NO, 1,I-diethyl-2-hydroxy-2-nitrosohydrazine; DETC, diethyldithio carbamate; EPR, electron paramagnetic resonance; Hb, hemo globin; L-NAME, N-nitro-L-arginine methyl ester; NMDA, N-methyl-o-aspartate; NOS, nitric oxide synthase. 715overlapping Cu-DETC signals. By perfusing various con centrations of an NO-generating agent, 1, I-diethyl-2-hy droxy-2-nitrosohydrazine, into the rat brains, the amount of the "trapped NO" was calibrated. The size of the NO Fe-DETC signal was well correlated with the NO con centrations in the perfusate (correlation coefficient r = 0.998, P < 0.01). Based on this calibration curve, it was found that the amount of trapped NO during forebrain ischemia increased to seven times that of the control (control n = 5, forebrain ischemia n = 4, P < 0.005). Key Words: Brain ischemia-Diethyldithiocarbamate-l, 1-Di ethyl-2-hydroxy-2-nitrosohydrazine-Electron paramag netic resonance-Nitric oxide-Nitric oxide trapping.1992; Buchanan and Phillis, 1993;Pelligrino et al. , 1993). The neuronal production of NO is evidenced by the fact that a constitutive NO synthase (NOS) exists in neurons throughout the brain Vincent and Kimura, 1992;Nozaki et al., 1993). NO acts as an intraneuronal and interneu ronal messenger by activating soluble guanylate cy clase and by blocking the N-methyl-o-aspartate (NMDA) receptor (Knowles et al., 1989; Garth waite, 1991;Bredt and Snyder, 1992;Lei et al., 1992;Manzoni et al., 1992). NO is a free radical, and its short half-life «30 s) has rendered direct measurement difficult. To demonstrate the produc tion of NO indirectly, the levels of nitrite sjnitrates, stable metabolites of NO, and cyclic GMP have been measured. Alternatively, NOS inhibi...

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“…This signal is characteristic of NO complexes of various iron-sulfur units containing proteins, peptides and chelates, including purified succinate dehydrogenase. 22) Inhibition of the phosphoroclastic system was observed after the addition of NO, but such a result would be expected from any process that is dependent on iron-sulfur centers. 22) The addition of NaNO 2 to a C. botulinum vegetative cell suspension results in the formation of iron-NO complexes.…”

Section: Discussionmentioning

confidence: 95%

“…22) Inhibition of the phosphoroclastic system was observed after the addition of NO, but such a result would be expected from any process that is dependent on iron-sulfur centers. 22) The addition of NaNO 2 to a C. botulinum vegetative cell suspension results in the formation of iron-NO complexes. Since iron-sulfur proteins exist in E. coli 23) and the EPR signal from NaNO 2 -treated cells of E. coli O157:H7 was identical to that of C. botulinum, it appears that iron-NO complexes were also formed in NaNO 2 -treated E. coli O157:H7.…”

Section: Discussionmentioning

confidence: 95%

“…22) Woods et al 8) have reported a finding suggesting such a mechanism, including the formation of a small but detectable amount of NO after the addition of nitrite to a cell-free system. Although the modification of the iron-sulfur centers certainly resulted in an antibacterial effect, the addition of NaNO 2 may have exerted other inhibitory actions.…”

Section: Discussionmentioning

confidence: 99%

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Anti-microbial Action against VerotoxigenicEscherichia coliO157:H7 of Nitric Oxide Derived from Sodium Nitrite

Yoshikawa

Suzuki

Hisamatsu

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Tetranuclear and binuclear iron-sulfur clusters in succinate dehydrogenase: A method of iron quantitation by formation of paramagnetic complexes

Cited by 52 publications

References 12 publications

Stability of the nitrosyl—sirohaem complex of plant nitrite reductase, investigated by EPR spectroscopy

Stability of the nitrosyl—sirohaem complex of plant nitrite reductase, investigated by EPR spectroscopy

Electron Paramagnetic Resonance (EPR) Detection of Nitric Oxide Produced during Forebrain Ischemia of the Rat

Anti-microbial Action against VerotoxigenicEscherichia coliO157:H7 of Nitric Oxide Derived from Sodium Nitrite

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