Tetrazolo[1,5-a]quinoline moiety-based Os(IV) complexes: DNA binding/cleavage, bacteriostatic and photocytotoxicity assay

Pursuwani, Bharat H.; Bhatt, Bhupesh S.; Vaidya, Foram U.; Pathak, Chandramani; Patel, Mohan N.

doi:10.1080/07391102.2020.1756912

Cited by 8 publications

(1 citation statement)

References 42 publications

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“…The half-maximal inhibitory concentration (IC 50 ) was determined by the MTT assay method [39]. Briefly, 1.0 × 10 4 MCF-7 cells were treated with prepared 1.0, 2.5, 5.0, 10.0, 20.0, and 30.0 µg/mL of QCN, mixed P123/F88(2:1) micelles, and QCN-incorporated mixed P123/F88(2:1) micelles for 24 h. After that, the cells were washed with DPBS and incubated with MTT (5.0 mg/mL) for 4 h at 303 K in a dark environment.…”

Section: In Vitrocell Proliferation Activity Studiesmentioning

confidence: 99%

Formulation, Solubilization, and In Vitro Characterization of Quercetin-Incorporated Mixed Micelles of PEO-PPO-PEO Block Copolymers

Patel

Shaikh

Ray

et al. 2021

Appl Biochem Biotechnol

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Quercetin (QCN) is a plant polyphenol with a variety of medicinal effects. Poor water solubility, on the other hand, restricts its therapeutic effectiveness. The purpose of this study was to develop mixed micellar systems using two biocompatible amphiphilic PEO-PPO-PEO triblock copolymers, Pluronic P123 (EO 20 -PO 70 -EO 20 ) and Pluronic F88 (EO 104 -PO 39 -EO 104 ), in order to enhance the aqueous solubility and oral bioavailability of QCN drug. The critical micelle concentrations (CMCs) of mixed P123/F88 micellar solutions were investigated using UV-visible spectroscopy with pyrene as a probe. Mixed P123/F88 micelles have low CMCs, indicating that they have a stable micelle structure even when diluted. The solubility of QCN in aqueous mixed P123/F88 micellar solutions at different temperatures was investigated to better understand drug entrapment. The QCN solubility increased with increasing temperature in the mixed P123/F88 micellar system. The QCN-incorporated mixed P123/F88 micelles were prepared using the thin-film hydration method and were well characterized in terms of size and morphology, compatibility, in vitro release and antioxidant profile. In addition, the cell proliferation activity of the mixed micelles was evaluated in the MCF-7 cell line. The QCN-incorporated mixed P123/F88 micelles had a small particle size (< 25 nm) and a negative zeta potential with a spherical shape. The in vitro release behaviour of QCN from a mixed P123/F88 micellar system was slower and more sustained at physiological conditions. The oxidation resistance of QCN-incorporating mixed P123/F88 micelles was shown to be considerably higher than that of pure QCN. An in vitro cell proliferation study revealed that QCN-incorporated mixed micells were effective in inhibiting tumour cell growth. In conclusion, the QCNincorporated mixed P123/F88 micelle may be a promising approach to increase QCN oral bioavailability, antioxidant activity, and cell viability.

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Section: In Vitrocell Proliferation Activity Studiesmentioning

confidence: 99%