Tetrodes markedly improve the reliability and yield of multiple single-unit isolation from multi-unit recordings in cat striate cortex

Gray, Charles M.; Maldonado, Pedro E.; Wilson, Mathew W.; McNaughton, Bruce L.

doi:10.1016/0165-0270(95)00085-2

Cited by 668 publications

(521 citation statements)

References 16 publications

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“…Micro-electrode arrays (MEAs) (Thomas et al 1972;Gross 1979;Pine 1980) and related technologies such as tetrode probes (Gray et al 1995), silicon probes (Bai and Wise 2001) and multi-wire probes (Nicolelis et al 1998) offer great promise to record action potentials extracellularly from a large number of cells simultaneously (Meister et al 1994;Potter 2001), in cell culture, in slice or in vivo (Bragin et al 2000). In addition, electrical stimulation through such arrays has been reported in a wide variety of preparations, such as murine spinal cord (Gross et al 1993), rat cortex (Jimbo et al 1999), cat sciatic nerve (Branner and Normann 2000) and rabbit retina (Grumet et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Real-time multi-channel stimulus artifact suppression by local curve fitting

Wagenaar

Potter

2002

Journal of Neuroscience Methods

217

179

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We describe an algorithm for suppression of stimulation artifacts in extracellular micro-electrode array (MEA) recordings. A model of the artifact based on locally fitted cubic polynomials is subtracted from the recording, yielding a flat baseline amenable to spike detection by voltage thresholding. The algorithm, SALPA, reduces the period after stimulation during which action potentials cannot be detected by an order of magnitude, to less than 2 ms. Our implementation is fast enough to process 60-channel data sampled at 25 kHz in real-time on an inexpensive desktop PC. It performs well on a wide range of artifact shapes without re-tuning any parameters, because it accounts for amplifier saturation explicitly and uses a statistic to verify successful artifact suppression immediately after the amplifiers become operational. We demonstrate the algorithm's effectiveness on recordings from dense monolayer cultures of cortical neurons obtained from rat embryos. SALPA opens up a previously inaccessible window for studying transient neural oscillations and precisely timed dynamics in short-latency responses to electric stimulation.

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Section: Introductionmentioning

confidence: 99%

Real-time multi-channel stimulus artifact suppression by local curve fitting

Wagenaar

Potter

2002

Journal of Neuroscience Methods

217

179

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“…The principle of the tetrode recording technique was proposed by McNaughton et al (1983b) as an extension of the stereotrode recording method and has subsequently been described in detail (O'Keefe and Recce, 1993;Wilson and McNaughton, 1993;Gray et al, 1995;Gothard et al, 1996). Briefly, the tips of four microwires of a tetrode are so closely spaced that most cells recorded by any of the wires will simultaneously be recorded by one or more of the other wires, typically at a different amplitude.…”

Section: Recording Techniques and Proceduresmentioning

confidence: 99%

The Effect of Aging on Experience-Dependent Plasticity of Hippocampal Place Cells

et al. 1997

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The firing characteristics of 1437 CA1 pyramidal neurons were studied in relation to both spatial location and the phase of the theta rhythm in healthy young and old rats performing a simple spatial task on a rectangular track. The old rats had previously been found to be deficient on the Morris spatial learning task. Age effects on the theta rhythm per se were minimal. Theta amplitude and frequency during rapid eye movement sleep were virtually identical. During behavior, theta frequency was slightly reduced with age. In both groups, cell firing occurred at progressively earlier phases of the theta rhythm as the rat traversed the place field of the cell (i.e., there was "phase precession," as reported by others). The net phase shift did not differ between age groups. The main finding of the study was a loss of experience-dependent plasticity in the place fields of old rats. During the first lap around the track on each day, the initial sizes of the place fields were the same between ages; however, place fields of young rats, but not old, expanded significantly during the first few laps around the track in a given recording session. As the place fields expanded, the rate of change of firing with phase slowed accordingly, so that the net phase change remained constant. Thus changes in field size and phase precession are coupled. A deficit in plasticity of place fields in old rats may lead to a less accurate population code for spatial location.

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“…Tetrodes allow both simultaneous recordings of multiple neurons and identi®cation of each individual unit based on spike amplitude pro®le of each unit activity recorded across four electrode channels [13,16]. This technique, compared to other methods, has a number of advantages in recording multiple neighboring neurons with clear isolation of individual units [8]. Detailed description of recording procedures in the medial PFC has been reported [11].…”

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confidence: 99%