TGF-β Family Signaling in Mesenchymal Differentiation

Grafe, Ingo; Alexander, Stefanie; Peterson, Jonathan; Snider, Taylor Nicholas; Lévi, Benjamin; Lee, Brendan; Mishina, Yuji

doi:10.1101/cshperspect.a022202

Cited by 211 publications

(174 citation statements)

References 542 publications

(721 reference statements)

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“…Tgf-b signaling plays critical roles in cellular events such as embryonic development (Liu et al, 2018;Meyers and Kessler, 2017), homeostasis of hematopoietic stem cells (Blank and Karlsson, 2015), mesenchymal differentiation (Grafe et al, 2018), aging, and cancer (Papageorgis, 2017). Interestingly, modulation of the Tgf-b signaling in injured mice retina, with little regenerative capability, had a negligible effect on MG activation and regeneration (Kugler et al, 2015).…”

Section: Tgf-b Signaling Is Essential For Normal Retinal Regenerationmentioning

confidence: 99%

Biphasic Role of Tgf-β Signaling during Müller Glia Reprogramming and Retinal Regeneration in Zebrafish

et al. 2020

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Section: Tgf-b Signaling Is Essential For Normal Retinal Regenerationmentioning

confidence: 99%

Biphasic Role of Tgf-β Signaling during Müller Glia Reprogramming and Retinal Regeneration in Zebrafish

et al. 2020

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“…Transforming growth factor beta (TGFβ) plays an important role in determining bone mass and quality . Mice overexpressing TGFβ in bone have osteoporosis, whereas mice with genetic TGFβ inhibition have stronger bones .…”

Section: Treatmentmentioning

confidence: 99%

“…(115,116) Transforming growth factor beta (TGFb) plays an important role in determining bone mass and quality. (117) Mice overexpressing TGFb in bone have osteoporosis, (118) whereas mice with genetic TGFb inhibition have stronger bones. (119) TGFb signaling is increased in Crtap-/and G610C mice, and pharmacologic inhibition of TGFb with a neutralizing antibody led to higher bone mass and stronger bones.…”

Section: Bisphosphonatesmentioning

confidence: 99%

Osteogenesis Imperfecta: New Perspectives From Clinical and Translational Research

2019

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Osteogenesis imperfecta (OI) is a monogenic bone fragility disorder that usually is caused by mutations in one of the two genes coding for collagen type I alpha chains, COL1A1 or COL1A2 . Mutations in at least 18 other genes can also lead to an OI phenotype. As genetic testing is more widely used, mutations in these genes are also more frequently discovered in individuals who have a propensity for fractures, but who do not have other typical clinical characteristics of OI. Intravenous bisphosphonate therapy is still the most widely used drug treatment approach. Preclinical studies in OI mouse models have shown encouraging effects when the antiresorptive effect of a bisphosphonate was combined with bone anabolic therapy using a sclerostin antibody. Other novel experimental treatment approaches include inhibition of transforming growth factor beta signaling with a neutralizing antibody and the inhibition of myostatin and activin A by a soluble activin receptor 2B. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research

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“…Main coordinators of this process are transforming growth factor-β (TGF-β) family proteins and Wnt/β-catenin signaling [54,55]. In vitro, the mesenchymal condensation is mimicked through the self-assembly methods [56,57] with TGF-β supplementation.…”

Section: Mesenchymal Condensation: Necessary Requirement For Chondrogmentioning

confidence: 99%

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

Gadjanski

2018

Osteochondral Tissue Engineering

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In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue engineering for potential clinical use, other applications of biomimetic OC TE including formation of the OC tissues to be used as high-fidelity disease/ healing models and as in vitro models for drug toxicity/efficacy evaluation are covered. Highlights Biomimetic OC TE uses "smart" scaffolds able to locally regulate cell phenotypes and dual-flow bioreactors for two sets of conditions for cartilage/ bone Protocols for hierarchical OC grafts engineering should entail mesenchymal condensation for cartilage and vascular component for bone Immunomodulation, low oxygen tension, purinergic signaling, time dependence of stimuli application are important aspects to consider in biomimetic OC TE Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering Ivana GadjanskiAbstract In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue en...

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TGF-β Family Signaling in Mesenchymal Differentiation

Cited by 211 publications

References 542 publications

Biphasic Role of Tgf-β Signaling during Müller Glia Reprogramming and Retinal Regeneration in Zebrafish

Biphasic Role of Tgf-β Signaling during Müller Glia Reprogramming and Retinal Regeneration in Zebrafish

Osteogenesis Imperfecta: New Perspectives From Clinical and Translational Research

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

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