TGF‐β1‐induced EMT of non‐transformed prostate hyperplasia cells is characterized by early induction of SNAI2/Slug

Abstract

This study suggests that in BPH-1 cells the transcription factor SNAI2/Slug is important for EMT initiation, while the ZEB family of transcription factors in cooperation with the miR-200 family may oppose the reversal of the EMT phenotype.

Significance of E-cadherin, β-catenin, and vimentin expression as postoperative prognosis indicators in cervical squamous cell carcinoma

“…The main molecular factors triggering EMT are the nuclear factor κB pathway [11], the Wnts signaling pathway [12], the transforming growth factor β 1 pathway [13,14], the integrin family members [15,16], and the activation of receptor tyrosine kinases [4]. The central target of these signaling pathways is the repression of E-cadherin expression and the increase of vimentin expression.…”

WITHDRAWN: Mechanisms navigating the TGF-β pathway in prostate cancer

“…In PC-3 prostate cancer cells, blockade of NF-kB signaling leads to decreased vimentin expression and inhibition of their invasive capability, indicating functional involvement of NF-kB in mediating TGF-b-induced EMT [50]. Other transcription factors, such as SNAI2/Slug that control E-cadherin expression (epithelial cell marker), are causally involved in TGF-b1-induced EMT in non-transformed prostate cells, conferring loss of polarity at the invading front [51]. In benign epithelial cells, EMT transcriptional inducers, Snail and Twist, contribute to the appearance of CD44Hi/CD24low cancer stem cells phenotype, implicating EMT as the process driving acquisition of stemlike characteristics in cancer cells [52].…”

Transforming growth factor-β1 induces epithelial-mesenchymal transition and increased expression of matrix metalloproteinase-16 via miR-200b downregulation in bladder cancer cells

“…TGF-β1 is a cytokine that enhances cell characteristics associated with invasiveness, metastasis and motility (14,16). EMT is associated with progression and poor prognosis in various types of cancer, and can be induced by TGF-β1 (14). In a study by Koo et al (15), AY-27 rat bladder cancer cells were incubated with TGF-β1 and were observed to have reduced E-cadherin and increased vimentin immunoreactivity.…”

“…This may be a novel mechanism of invasion and metastasis in bladder cancer. TGF-β1 is a cytokine that enhances cell characteristics associated with invasiveness, metastasis and motility (14,16). EMT is associated with progression and poor prognosis in various types of cancer, and can be induced by TGF-β1 (14).…”

“…EMT is associated with progression and poor prognosis in numerous types of cancer, and EMT can be induced by TGF-β1 (14,15). In the present study, a time-course western blot analysis was designed to investigate the expression of EMT markers, including E-cadherin, N-cadherin and vimentin.…”