TGFB1 modulates in vitro secretory activity and viability of equine luteal cells

Galvão, António; Wołodko, Karolina; Rebordão, Maria Rosa; Skarzynski, Dariusz J.; Ferreira‐Dias, Graça

doi:10.1016/j.cyto.2018.03.038

Cited by 11 publications

(11 citation statements)

References 35 publications

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“…Similar events appear to take place in the CL undergoing luteolysis, as the consistent proliferation of luteal fibroblast was shown to be mediated by TGFβ1 in bovine CL (24). Importantly, our recent studies also revealed the effect of TGFβ1 on equine functional and structural luteolysis (3, 25), reiterating the importance of the TGFβ ligands Nodal and TGFβ1 for equine CL regression. Next, we treated the mid CL explants with the proangiogenic factors VEGFA and FGF, and found that only VEGFA treatment showed a trend on Alk7 downregulation.…”

Section: Discussionsupporting

confidence: 56%

“…During luteolysis, PGF 2α orchestrates the interactions between TSP1 and TGFβ1 (10). Our previous results demonstrated the importance of the crosstalk between PGF 2α and Nodal/ TGFβ1 (2, 3). Furthermore, the link between the main luteolysin and HIF1α was also shown to mediate functional and structural regression of the CL (8).…”

Section: Discussionmentioning

confidence: 79%

“…We have recently shown that Nodal enters a close feed-forward loop with PGF 2α toward progesterone (P4) downregulation and intraluteal PGF 2α amplification at the time of luteolysis initiation (2). Importantly, when we blocked Nodal and TGFβ1 signaling in PGF 2α treated cells we abolished its functional and structural luteolytic role (3). Furthermore, the regression of the corpus luteum (CL) is also associated with decreased blood flow (5, 6), which originates low oxygen (O 2 ) tension in the organ, an event named hypoxia (7).…”

Section: Introductionmentioning

confidence: 91%

“…Following the trigger of the uterine prostaglandin (PG) F 2α , various local auto-, paracrine interactions are initiated (1, 2). Amongst others, the morphogens from transforming growth factor-β (TGF β) superfamily Nodal and TGFβ1 appear to be key for luteolysis in the mare (3). Particularly Nodal, after binding to its type II receptor, the activin-A receptor 2B (ACVR2B), phosphorylates either activin-like kinase 4 (Alk4) or activin-like kinase 7 (Alk7), and subsequently mediates the phosphorylation of a SMAD family member 2 (Smad2) or Smad3, which finally translocates to the nucleus and regulates transcription (4).…”

Section: Introductionmentioning

confidence: 99%

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The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum

et al. 2019

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The regulation of corpus luteus (CL) luteolysis is a complex process involving a myriad of factors. Previously, we have shown the involvement of Nodal in functional luteolysis in mares. Presently, we ask the extent of which Nodal mediation of luteolysis is done through regulation of angioregression. We demonstrated the interaction between Nodal and hypoxia-inducible factor 1 α (HIF1α) and thrombospondin 1/thrombospondin receptor (TSP1/CD36) systems, could mediate angioregression during luteolysis. First, we demonstrated the inhibitory effect of Nodal on the vascular marker platelet/endothelial cell adhesion molecule 1 (CD31). Also, treatment of mid CL explants with vascular endothelial growth factor A (VEGFA) showed a trend on activin-like kinase 7 (Alk7) protein inhibition. Next, Nodal was also shown to activate HIF1α and in vitro culture of mid CL explants under decreased oxygen level promoted Nodal expression and SMAD family member 3 (Smad3) phosphorylation. In another experiment, the crosstalk between Nodal and TSP1/CD36 was investigated. Indeed, Nodal increased the expression of the anti-angiogenic TSP1 and its receptor CD36 in mid CL explants. Finally, the supportive effect of prostaglandin F2α (PGF2α) on TSP1/CD36 was blocked by SB431542 (SB), a pharmacological inhibitor of Nodal signaling. Thus, we evidenced for the first time the in vitro interaction between Nodal and both HIF1α and TSP1 systems, two conserved pathways previously shown to be involved in vascular regression during luteolysis. Considering the given increased expression of Nodal in mid CL and its role on functional luteolysis, the current results suggest the additional involvement of Nodal in angioregression during luteolysis in the mare, particularly in the activation of HIF1α and TSP1/CD36.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum

et al. 2019

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“…Primers were designed using Primer 3.0 v.0.4.0. software [28], based on gene sequences from GeneBank (NCBI), as described before [29]. All primers were synthesised by Sigma Aldrich.…”

Section: Real-time Polymerase Chain Reactionmentioning

confidence: 99%

Leptin Resistance in the Ovary of Obese Mice is Associated with Profound Changes in the Transcriptome of Cumulus Cells

Wołodko

Walewska

Adamowski

et al. 2020

Cell Physiol Biochem

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Endometrial and luteal gene expression of putative gene regulators of the equine maternal recognition of pregnancy

Amorim

Bramer

Rajamanickam

et al. 2022

Animal Reproduction Science

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TGFB1 modulates in vitro secretory activity and viability of equine luteal cells

Cited by 11 publications

References 35 publications

The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum

The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum

Leptin Resistance in the Ovary of Obese Mice is Associated with Profound Changes in the Transcriptome of Cumulus Cells

Endometrial and luteal gene expression of putative gene regulators of the equine maternal recognition of pregnancy

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