Thalassiosira pseudonana and Skeletonema costatum biomass optimization: Cultivation, harvesting, extraction of oils and biodiesel and pelletization of the residue

Iglesias, Silvia Calvo; Míguez, Clara; Sánchez, Ángel; Cancela, Ángeles; Álvarez, Xana

doi:10.1016/j.seares.2022.102243

Cited by 2 publications

(1 citation statement)

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“…Nevertheless, there is little information on the cultivation potential and chemical profile of the genus Skeletonema. Some species such as S. marinoii, S. dohrnii and S. costatum have been previously studied [7,[16][17][18]; however, there are no reports on S. gravileii. In a nutrient-deprived environment, Skeletonema species are able to increase lipid production [10], and even produce higher amounts of omega-3 eicosapentaenoic acid (EPA) [19].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Growth and Chemical Profile of Diatom Skeletonema grevillei in Bioreactor and Incubation-Shaking Cabinet in Two Growth Phases

et al. 2022

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Marine microalgae, diatoms, are considered a source of a wide range of high-value compounds, and numerous studies indicate their biotechnological potential in the food and feed industry, cosmetic industry, nanotechnology, pharmaceutical industry, biodiesel production, fertilizers, and wastewater treatment. The aim of this study was to compare the growth, chemical profiles, and antioxidant activity of the diatom Skeletonema grevillei cultivated in a bioreactor and an incubation-shaking cabinet at different growth phases (after 192 and 312 h). Growth was monitored by evaluating cell density with the Sedgewick Rafter chamber, and the collected biomass was extracted with 70% ethanol assisted by ultrasound. Extracts were evaporated to dryness and compounds were identified in derivatized form by gas chromatography and mass spectrometry (GC-MS) analysis, while antioxidant capacity was evaluated by DPPH and ORAC. Significantly faster growth was observed in the bioreactor than in the incubation-shaking cabinet. Oleamide, palmitelaidic acid, glycerol monostearate, myristic acid, cholesterol, eicosapentaenoic acid, 1-monopalmitin, and 24-methylene cholesterol were identified as the major compounds in both systems. Among them, oleamide was the dominant compound in both systems. It is also shown that prolonging the cultivation period had a direct effect on increasing the extract yield. The highest DPPH inhibition (11.4 ± 1%) and ORAC values (93.3 ± 8.4 mM TE) were obtained for the S. grevillei extract recovered from the bioreactor after 312 h. The obtained results contribute to the possibility of using S. grevillei for various biotechnological applications in the future.

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