The 14.6 kd rubber elongation factor (Hev b 1) and 24 kd (Hev b 3) rubber particle proteins are recognized by IgE from patients with spina bifida and latex allergy

Yeang, Hoong Yeet; Cheong, Kay; Elumalai, Sunderasan; Hamzah, Samsidar; Chewa, Nyu Ping; Hamidb, Sharifah; Hamilton, Robert G.; Cardosa, Mary Jane

doi:10.1016/s0091-6749(96)70097-0

Cited by 145 publications

(108 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown in Fig. 8 To further confirm that SRPP plays a role in rubber biosynthesis, polyclonal antibodies from SRPP-challenged rabbit serum and monoclonal antibodies from mouse were prepared (13), and their ability to inhibit rubber biosynthesis in C-serum was tested. The untreated blood serum and the culture supernatant were used as a control without further purification.…”

Section: Resultsmentioning

confidence: 98%

“…Siler et al (27) analyzed the composition of rubber particles of four rubber-producing plants including H. brasiliensis and suggested that the rubber particle surface is a mosaic of protein, conventional membrane lipids, and other components. Among many latex proteins, two main proteins remain associated with rubber particles after repeated washing: REF (14 kDa) with large rubber particles and SRPP (23 kDa) with small rubber particles (13).…”

Section: Discussionmentioning

confidence: 99%

“…For PCR, 10 l of the first strand cDNA synthesis was used to amplify part of the coding sequence for SRPP. A degenerate primer, BW1, was designed according to sequence similarities between Hev b 1 (commonly known as REF) and previously published peptide fragments of Hev b 3 (13). The peptide sequence KYLDFV was chosen (see Fig.…”

Section: Methodsmentioning

confidence: 99%

“…One of the genes most abundantly expressed in latex was found to be a cDNA clone encoding a major rubber particle protein, Hev b 3. This protein causes allergenic response in spina bifida patients, and only its partial amino acid sequence was determined (13,18). The gene encoding this protein has not been cloned.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Isolation, Characterization, and Functional Analysis of a Novel cDNA Clone Encoding a Small Rubber Particle Protein from Hevea brasiliensis

Kang

Shin

et al. 1999

Journal of Biological Chemistry

Self Cite

193

118

View full text Add to dashboard Cite

Biochemical evidence reported so far suggests that rubber synthesis takes place on the surface of rubber particles suspended in the latex of Hevea brasiliensis. We have isolated and characterized a cDNA clone that encodes a protein tightly bound on a small rubber particle. We named this protein small rubber particle protein (SRPP). Prior to this study, this protein was known as a latex allergen, and only its partial amino acid sequence was reported. Sequence analysis revealed that this protein is highly homologous to the rubber elongation factor and the Phaseolus vulgaris stress-related protein. Southern and Northern analyses indicate that the protein is encoded by a single gene and highly expressed in latex. An allergenicity test using the recombinant protein confirmed that the cloned cDNA encodes the known 24-kDa latex allergen. Neither ethylene stimulation nor wounding changed the transcript level of the SRPP gene in H. brasiliensis. An in vitro rubber assay showed that the protein plays a positive role in rubber biosynthesis. Therefore, it is likely that SRPP is a part of the rubber biosynthesis machinery, if not the rubber polymerase, along with the rubber elongation factor.Rubber (cis-1,4-polyisoprene), an isoprenoid polymer with no known physiological function to the plant, is produced in about 2000 plant species with varying degrees of quality and quantity (1). Rubber is the raw material of choice for heavy duty tires and other industrial uses requiring elasticity, flexibility, and resilience. Hevea brasiliensis has been the only commercial source of natural rubber mainly because of its abundance in the tree, its quality, and the ease of harvesting. The diminishing acreage of rubber plantations and life-threatening latex allergy to Hevea rubber, coupled with an increasing demand, have prompted research interests in the study of rubber biosynthesis and the development of alternative rubber sources.In H. brasiliensis, rubber synthesis takes place on the surface of rubber particles suspended in the latex (the cytoplasm of laticifers). The laticifers are specialized vessels that are located adjacent to the phloem of the rubber tree. When severed during tapping, the high turgor pressure inside the laticifers expels latex containing 30 -50% (w/w) cis-1,4-polyisoprene. The latex can be fractionated by centrifugation into three phases: the top fraction containing mostly rubber particles, the metabolically active middle fraction (called C-serum), and the bottom fraction of mainly vacuole-like organelles called lutoids. More than 240 expressed sequence tags (ESTs) 1 have been identified from the latex of H. brasiliensis 2 Kush et al. (2) have shown differential expression of several rubber biosynthesis-related genes in latex. The rubber elongation factor (REF), an enzyme involved in rubber biosynthesis (3), is highly expressed in laticifers (4). Laticiferous cells actively translate the transcribed genes into proteins. About 200 distinct polypeptides are present in the latex of H. brasiliensis (5). Arokiaraj et al...

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Isolation, Characterization, and Functional Analysis of a Novel cDNA Clone Encoding a Small Rubber Particle Protein from Hevea brasiliensis

Kang

Shin

et al. 1999

Journal of Biological Chemistry

Self Cite

193

118

View full text Add to dashboard Cite

show abstract

“…allergen.org/index.php). Hev b 1, Rubber elongation factor (HbREF, 14.7 kDa) and Hev b 3, Small rubber particle protein (HbSRPP, 22.3 kDa), are recognized as major allergens found in Hevea latex [28][29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition

Dafoe¹,

Huang²,

Yang³

2017

J Biotechnol Biomater

View full text Add to dashboard Cite

The annual plant Taraxacum kok-saghyz, commonly called Russian dandelion, has recently gained commercial attention as an alternative source to the rubber tree, Hevea brasiliensis, for natural rubber latex. While producing rubber of equivalent quality, the potential application of TKS latex for hypoallergenic products remains to be determined. In order to quantify the allergenicity of TKS latex proteins, we compared the extent of anti-Hevea latex polyclonal and monoclonal antibody cross-reactivity toward Hevea latex proteins to that of TKS latex proteins by quantitative ELISA methods and semi-quantitative image densitometry. ELISA measurement of polyclonal antibody recognition toward TKS latex proteins was on the order of one-tenth relative to equal amounts of Hevea latex protein, while recognition by the monoclonal antibody was below 2%. Immunoblots confirmed significantly reduced polyclonal antibody recognition toward TKS latex proteins, in the same range as the cross-reactivity exhibited toward the rubber-producing plant, Lactuca sativa and Glycine max, a non-rubber-producing plant. Despite the presence of cross-reactivity, these quantitative results support TKS as an alternative source of latex that may require lessintensive processing for the reduction of antigenic proteins in the manufacture of hypoallergenic natural rubber latex products.

show abstract

Natural Rubber Latex Allergy

Brehler¹,

Kütting²

2001

Arch Intern Med

View full text Add to dashboard Cite

In the past 10 years, IgE-mediated allergy to natural rubber latex has become a significant health problem in industrialized countries, especially among health care workers, patients with congenital malformations, and children with a history of multiple surgical interventions. Curative treatment inducing immunological tolerance in formerly sensitized patients is experimental and not yet generally available. Therefore, it is important to be aware of the seriousness of latex allergy and to understand the risk factors leading to this allergy. Preventive measures are needed to decrease the incidence of natural rubber latex sensitization. This article gives a brief review of the current state of knowledge concerning latex allergy, including a definition of latex, epidemiological data, identified allergens, the clinical spectrum, diagnostic procedures, cross-reactions, preventive measures, the legislative background, and economics.

show abstract

The 14.6 kd rubber elongation factor (Hev b 1) and 24 kd (Hev b 3) rubber particle proteins are recognized by IgE from patients with spina bifida and latex allergy

Cited by 145 publications

References 20 publications

Isolation, Characterization, and Functional Analysis of a Novel cDNA Clone Encoding a Small Rubber Particle Protein from Hevea brasiliensis

Isolation, Characterization, and Functional Analysis of a Novel cDNA Clone Encoding a Small Rubber Particle Protein from Hevea brasiliensis

Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition

Natural Rubber Latex Allergy

Contact Info

Product

Resources

About