The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding

Abstract

Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds given the extreme “crowding” conditions within the mitochondrion. Here, we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA. We use high molecular mass polyethylene glycol as a macromolecular cosolute and monitor the structure of the RNA globally and with nucleotide resolution. We demonstrate that crowding has no impact on the 2D fold and we conclude that the MFE structure in dilute solvent conditions represents a good proxy for the folding of the pre-mRNA in its mitochondrial solvent context.

Comparative Genomics and Phylogenetic Analysis of the Chloroplast Genomes in Three Medicinal Salvia Species for Bioexploration

“…The rps 12 gene was split into two introns; one intron between exon 2 and 3 was 528 bp in length, and another intron between exon 1 and 2 was about 28 kb in length ( Table 2 , Figure S2 ). The latter intron is trans-spliced to produce mature rps 12 mRNA ( Figure S2 ) [ 36 ]. The exon 1 and the two copies of exons are trans-spliced together to form two transcripts.…”

Comparative genomics and phylogenetic analysis of the chloroplast genomes in three medicinal Salvia species for bioexploration

“…The rps12 gene is splitted by two introns; one intron between exon 2 and 3 is 528 bp in length, another intron between exon 1 and 2 is about 28 kb in length(table 2, additional 8, Figure S2). The latter intron is trans-spliced to produce mature rps12 mRNA (additional 8, Figure S2) [42]. The exon1 and the two copies of exons are trans-spliced together to form two transcripts.…”