The 3R approach to experimental dermatology

Sebaceous gland: Milestones of 30‐year modelling research dedicated to the “brain of the skin”

“…The growing interest in 3D spheroids is also due to the fact that this novel approach holds, like cell cultures did previously, great promise in terms of the replacement of animal experiments. [55,56] SZ95 sebocytes were cultured on a layer of Matrigel ® in Dulbecco's modified Eagle's medium containing epidermal growth factor and Matrigel ® . After 2 weeks, in vitro-derived 3D cell aggregates could be defined as spheroids.…”