The A78V Mutation in the Mad3-like Domain of<i>Schizosaccharomyces pombe</i>Bub1p Perturbs Nuclear Accumulation and Kinetochore Targeting of Bub1p, Bub3p, and Mad3p and Spindle Assembly Checkpoint Function

Kadura, Sheila; He, Xiangwei; Vanoosthuyse, Vincent; Hardwick, Kevin; Sazer, Shelley

doi:10.1091/mbc.e04-07-0558

Cited by 26 publications

(36 citation statements)

References 51 publications

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“…Imp1p-GFP, produced from the imp1 promoter at the endogenous locus, had a similar localization pattern ( Figure 3F-2) whereas GFP alone equilibrated across the nuclear envelope ( Figure 3F-1), as previously reported (Kadura et al 2005).…”

Section: Resultssupporting

confidence: 82%

The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

et al. 2005

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The nuclear import of classical nuclear localization signal-containing proteins depends on importin-a transport receptors. In budding yeast there is a single importin-a gene and in higher eukaryotes there are multiple importin-a-like genes, but in fission yeast there are two: the previously characterized cut15 and the more recently identified imp1. Like other importin-a family members, Imp1p supports nuclear protein import in vitro. In contrast to cut15, imp1 is not essential for viability, but imp1D mutant cells exhibit a telophase delay and mild temperature-sensitive lethality. Differences in the cellular functions that depend on Imp1p and Cut15p indicate that they each have unique physiological roles. They also have common roles because the imp1D and the cut15-85 temperature-sensitive mutations are synthetically lethal; overexpression of cut15 partially suppresses the temperature sensitivity, but not the mitotic delay in imp1D cells; and overexpression of imp1 partially suppresses the mitotic defect in cut15-85 cells but not the loss of viability. Both Imp1p and Cut15p are required for the efficient nuclear import of both an SV40 nuclear localization signal-containing reporter protein and the Pap1p component of the stress response MAP kinase pathway. Imp1p and Cut15p are essential for efficient nuclear protein import in S. pombe.

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Section: Resultssupporting

confidence: 82%

The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

et al. 2005

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“…For example, Bub1p binds to Bub3p throughout the cell cycle [reviewed in [Howell et al, 2000[Howell et al, , 2004Shah et al, 2004]. The association of SAC proteins with kinetochores is important for SAC function [Kadura et al, 2005;Taylor et al, 1998;Vanoosthuyse et al, 2004] perhaps because it alters the binding interactions between SAC proteins and influences the formation of the mitotic checkpoint complex (MCC) [Sudakin et al, 2001], which imposes a cell cycle arrest by binding to the Anaphase Promoting Complex (APC). The APC/C is a multi-protein ubiquitin ligase that targets important cell cycle proteins for degradation by the 26S proteasome.…”

Section: A Consensus Model For Spindle Checkpoint Functionmentioning

confidence: 99%

“…How does microtubule capture by the kinetochore displace SAC proteins, and how does kinetochore binding of SAC proteins influence the formation of the APC/Cinhibitory mitotic checkpoint complex? Nuclear accumulation of SAC proteins during interphase is crucial for SAC function in yeast and is also likely to be important in higher eukaryotes [Campbell and Hardwick, 2003;Campbell et al, 2001;Chen et al, 1998;Kadura et al, 2005;Ouyang et al, 1998;Yoon et al, 2004]. Although Bub1p has a functional NLS [Kadura et al, 2005] and other checkpoint proteins have putative NLS consensus sequences, the mechanism by which the nuclear accumulation of SAC proteins is accomplished or regulated has not been well studied.…”

Section: Future Frontiersmentioning

confidence: 99%

“…Nuclear accumulation of SAC proteins during interphase is crucial for SAC function in yeast and is also likely to be important in higher eukaryotes [Campbell and Hardwick, 2003;Campbell et al, 2001;Chen et al, 1998;Kadura et al, 2005;Ouyang et al, 1998;Yoon et al, 2004]. Although Bub1p has a functional NLS [Kadura et al, 2005] and other checkpoint proteins have putative NLS consensus sequences, the mechanism by which the nuclear accumulation of SAC proteins is accomplished or regulated has not been well studied. In addition, SAC proteins may have cellular functions distinct from their characterized roles in checkpoint signaling [reviewed in Gillett et al, 2004;Lew and Burke, 2003;Vanoosthuyse et al, 2004;Yu and Tang, 2005], and investigating these roles will be an important topic for future study.…”

Section: Future Frontiersmentioning

confidence: 99%

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SAC‐ing mitotic errors: How the spindle assembly checkpoint (SAC) plays defense against chromosome mis‐segregation

Kadura

Sazer

2005

Cell Motil. Cytoskeleton

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“…The Bub3 protein physically interacts with Bub1 in vivo in several organisms (Roberts et al 1994;Taylor et al 1998;Vanoosthuyse et al 2004). This interaction is required for the localization of these proteins to the kinetochores in both perturbed and unperturbed mitoses (Taylor et al 1998;Kadura et al 2005). Bub3 is a stoichiometric component of the mitotic checkpoint complex (Hardwick et al 2000;Sudakin et al 2001;Poddar et al 2005).…”

mentioning

confidence: 99%

Schizosaccharomyces pombeBub3 Is Dispensable for Mitotic Arrest Following Perturbed Spindle Formation

Tange

Niwa

2008

Genetics

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The core proteins of the spindle assembly checkpoint (SAC), Mads, Bubs, and Mps1, first identified in the budding yeast, are thought to be functionally and structurally conserved through evolution. We found that fission yeast Bub3 is dispensable for SAC, as bub3 null mutants blocked mitotic progression when spindle formation was disrupted. Consistently, the bub3 mutation only weakly affected the stability of minichromosome Ch16 compared with other SAC mutants. Fission yeast Rae1 has sequence homology with Bub3. The bub3 rae1 double mutant and rae1 single mutant did not have defective SAC, suggesting that these genes do not have overlapping roles for SAC. Observations of living cells revealed that the duration of the mitotic prometaphase/metaphase was longer in the bub3 mutant and was Mad2 dependent. Further, the bub3 mutant was defective in sister centromere association during metaphase. Together, these findings suggest that fission yeast Bub3 is required for normal spindle dynamics, but not for SAC.

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The A78V Mutation in the Mad3-like Domain ofSchizosaccharomyces pombeBub1p Perturbs Nuclear Accumulation and Kinetochore Targeting of Bub1p, Bub3p, and Mad3p and Spindle Assembly Checkpoint Function

Cited by 26 publications

References 51 publications

The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

SAC‐ing mitotic errors: How the spindle assembly checkpoint (SAC) plays defense against chromosome mis‐segregation

Schizosaccharomyces pombeBub3 Is Dispensable for Mitotic Arrest Following Perturbed Spindle Formation

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