The AAPT1 gene of soybean complements a cholinephosphotransferase-deficient mutant of yeast.

Dewey, Ralph E.; Wilson, Richard F.; Novitzky, William P.; Goode, John H.

doi:10.1105/tpc.6.10.1495

Cited by 66 publications

(44 citation statements)

References 49 publications

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“…In IMET1, the CCT gene was slightly up-regulated at 12 h under N − conditions (p < 0.001), whereas PECT was transcriptionally unchanged. IMET1 harbors two copies of the plant-type DAG:CDP-alchohol (choline/ethanolamine) phosphotransferase (DAG-CPT/EPT), which putatively encodes a bifunctional enzyme that can incorporate a CDP-choline or a CDP-ethanolamine into the DAG backbone CDP-CPT/EPT (Dewey et al, 1994;Qi et al, 2003). Of the two genes, g4589 was transiently up-regulated at 12 h (p < 0.001), but transcripts were reduced 2-fold at 48 h following N deprivation (Figure 4).…”

Section: Transcriptional Regulation Of Membrane Glycerolipids Biosyntmentioning

confidence: 99%

Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation

Han

Jia

et al. 2017

Front. Mar. Sci.

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HIGHLIGHTS• An electrospray ionization mass spectrometry-based lipidomics method was developed and integrated with transcriptomics to elucidate metabolic remodeling and turnover of microalgal membrane lipids by using Nannochloropsis oceanica as a model.The lack of lipidome analytical tools has limited our ability to gain new knowledge about lipid metabolism in microalgae, especially for membrane glycerolipids. An electrospray ionization mass spectrometry-based lipidomics method was developed for Nannochloropsis oceanica IMET1, which resolved 41 membrane glycerolipids molecular species belonging to eight classes. Changes in membrane glycerolipids under nitrogen deprivation and high-light (HL) conditions were uncovered. The results showed that the amount of plastidial membrane lipids including monogalactosyldiacylglycerol, phosphatidylglycerol, and the extraplastidic lipids diacylglyceryl-O-4 ′ -(N, N, N,-trimethyl) homoserine and phosphatidylcholine decreased drastically under HL and nitrogen deprivation stresses. Algal cells accumulated considerably more digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerols under stresses. The genes encoding enzymes responsible for biosynthesis, modification and degradation of glycerolipids were identified by mining a time-course global RNA-seq data set. It suggested that reduction in lipid contents under nitrogen deprivation is not attributable to the retarded biosynthesis processes, at least at the gene expression level, as most genes involved in their biosynthesis were unaffected by nitrogen supply, yet several genes were significantly up-regulated. Additionally, a conceptual eicosapentaenoic acid (EPA) biosynthesis network is proposed based on the lipidomic and transcriptomic data, which underlined import of EPA from cytosolic glycerolipids to the plastid for synthesizing EPA-containing chloroplast membrane lipids.

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Section: Transcriptional Regulation Of Membrane Glycerolipids Biosyntmentioning

confidence: 99%

Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation

Han

Jia

et al. 2017

Front. Mar. Sci.

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“…Genomic DNA and mRNAs were isolated from soybean and transferred to nylon membranes as previously described (Dewey et al, 1994). 32P-labeled DNA probes were synthesized using random primers according to the method of Feinberg and Vogelstein (1983).…”

Section: Northern and Southern Blot Analysesmentioning

confidence: 99%

Characterization of Soybean Choline Kinase cDNAs and Their Expression in Yeast and Escherichia coli

1996

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An expressed sequence tag from Arabidopsis that displayed sequence homology t o mammalian and yeast choline kinases was used t o isolate choline kinase-like cDNAs from soybean (Glycine max 1.). Two distinct cDNAs, designated GmCKl and CmCK2, were recovered that possessed full-length reading frames, each sharing approximately 32% identity at the predicted amino acid leve1 with the rat choline kinase. A third unique choline kinase-like cDNA, CmCK3, was also identified but was not full length. Heterologous expression of CmCKl in yeast (Saccharomyces cerevisiae) and CmCK2 i n both yeast and Escherichia coli demonstrated that each encodes choline kinase activity. I n addition to choline, other potential substrates for the choline kinase enzyme include ethanolamine, monomethylethanolamine (MME), and dimethylethanolamine (DME). 60th soybean choline kinase isoforms demonstrated negligible ethanolamine kinase activity. Competitive inhibition assays, however, revealed very distinct differences i n their responses t o DME and MME. DME effectively inhibited only the CmCK2-encoded choline kinase activity. Although MME failed to effectively inhibit either reaction, an unexpected enhancement of choline kinase activity was observed specifically with the CmCKl -encoded enzyme. These results show that choline kinase is encoded by a small, multigene family in soybean comprising two or more distinct isoforms that exhibit both similarities and differences with regard to substrate specificity.

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“…P-Etn is a common substrate for the biosynthesis of both PtdEtn and PtdCho: PtdEtn is produced via cytidine diphosphoethanolamine by CTP:phosphorylethanolamine cytidylyltransferase (PECT) (Mizoi et al, 2006) and amino alcohol:aminophosphotransferase (Dewey et al, 1994), whereas PtdCho synthesis requires trimethylation of P-Etn to P-Cho catalyzed by S-adenosyl-L-methionine: phosphoethanolamine N-methyltransferase (PMT) (Bolognese and McGraw, 2000). P-Cho is converted by CTP:phosphorylcholine cytidylyltransferase to cytidine diphospho-choline (Inatsugi et al, 2002), which is then incorporated into the DAG backbone by amino alcohol:aminophosphotransferase to produce PtdCho.…”

Section: Introductionmentioning

confidence: 99%

The Choline/Ethanolamine Kinase Family in Arabidopsis: Essential Role of CEK4 in Phospholipid Biosynthesis and Embryo Development

2015

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The AAPT1 gene of soybean complements a cholinephosphotransferase-deficient mutant of yeast.

Cited by 66 publications

References 49 publications

Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation

Metabolic Remodeling of Membrane Glycerolipids in the Microalga Nannochloropsis oceanica under Nitrogen Deprivation

Characterization of Soybean Choline Kinase cDNAs and Their Expression in Yeast and Escherichia coli

The Choline/Ethanolamine Kinase Family in Arabidopsis: Essential Role of CEK4 in Phospholipid Biosynthesis and Embryo Development

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