The Ability of Resveratrol to Attenuate Ovalbumin-Mediated Allergic Asthma Is Associated With Changes in Microbiota Involving the Gut-Lung Axis, Enhanced Barrier Function and Decreased Inflammation in the Lungs

Abstract

Asthma is a chronic respiratory disease highly prevalent worldwide. Recent studies have suggested a role for microbiome-associated gut–lung axis in asthma development. In the current study, we investigated if Resveratrol (RES), a plant-based polyphenol, can attenuate ovalbumin (OVA)-induced murine allergic asthma, and if so, the role of microbiome in the gut–lung axis in this process. We found that RES attenuated allergic asthma with significant improvements in pulmonary functions in OVA-exposed mice when tested using plethysmography for frequency (F), mean volume (MV), specific airway resistance (sRaw), and delay time(dT). RES treatment also suppressed inflammatory cytokines in the lungs. RES modulated lung microbiota and caused an abundance of Akkermansia muciniphila accompanied by a reduction of LPS biosynthesis in OVA-treated mice. Furthermore, RES also altered gut microbiota and induced enrichment of Bacteroides acidifaciens significantly in the colon accompanied by an increase in butyric acid concentration in the colonic contents from OVA-treated mice. Additionally, RES caused significant increases in tight junction proteins and decreased mucin (Muc5ac) in the pulmonary epithelium of OVA-treated mice. Our results demonstrated that RES may attenuate asthma by inducing beneficial microbiota in the gut-lung axis and through the promotion of normal barrier functions of the lung.

The Nutritional Intervention of Resveratrol Can Effectively Alleviate the Intestinal Inflammation Associated With Celiac Disease Induced by Wheat Gluten

“…Resveratrol abrogated the adverse effects of the gluten protein-derived peptide p31-43 and inhibited the overexpression of TG2 and inflammatory mediators iNOS and COX-2, thereby ameliorating dysfunction of cell barriers. This was consistent with Alharris’ study ( 31 ) that resveratrol could directly enhance the solidity of the epithelial barrier against the LPS-induced barrier disruption by increasing the expression of tight junction proteins. We identified an association of oxidative stress damage with the pathogenesis of celiac disease, where barrier function of the intestinal epithelium was disrupted by the upregulated oxidative stress in the tissues, which in turn promoted the development of the disease.…”

Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway

“…To validate the PCR results of upregulated mTOR gene expression, we performed immunofluorescent staining of lung tissue. We calculated the corrected total cell fluorescence (CTCF) for mTOR-stained cells in the lung tissue of naïve, SEB + VEH, and SEB + RES groups using ImageJ software from NIH, we performed immunofluorescent staining as described earlier (Alharris et al, 2018;Sarkar et al, 2019;Alharris et al, 2022). Briefly, the slides with a section of lung tissue were first deparaffinized according to the standard protocol, antigen…”

Probiotics: Protecting Our Health from the Gut

“…In addition, an increase in tight junction protein expression in alveolar epithelial cells and a decrease in LPS biosynthesis and mucin production were observed. This, in turn, led to an increase in the abundance of beneficial bacterial species in the gut, such as Bacteroides acidifaciens , and, consequently, an increase in butyric acid concentration [ 226 ] ( Figure 3 ).…”