IntroductionA DISTINGUISHED industrial chemist once stated that many of the present shortcomings in our knowledge of plant nutrition were largely due to Lawes and Gilbert having been too successful.Before the middle of the last century outstanding practical results were already being achieved by agricultural chemists who analysed soils and plants by relatively simple procedures and thereby deduced how the nutritional requirements of crops could be more adequately met. At that time knowledge of the physiological processes which take place in plants was meagre and, when plant physiology became a subject of active and precise experimentations, the study of nutritional relationships in the field had become so much the province of agricultural chemists that plant physiologists usually turned their attention elsewhere. In subsequent decades there was often surprisingly little contact between those who studied the physiology of nutrition, frequently in fragments of plant tissues grown in artificial media, and the agricultural chemist who grappled with the more immediately practical problems of evaluating the availability of nutrients in the soil and devising how nutritional conditions could be ameliorated.In this paper it is assumed that emphasis shall be placed on those characteristics of the behaviour of plants which are relevant to determining the relationship between nutrient absorption and the exchangeability of ions in the soil.The thought behind this remark was obvious.
Entry of nutrients into plantsNutrients enter plants as dissociated ions in solution ; the pathways which they follow are similar to those traversed by water, but the mechanism responsible for their movement is different. The driving force behind the transpiration stream is the loss of water from leaves ; mass flow occurs across the root. It is, however, beyond doubt that ions reach the conducting tissues of roots only after they have been conveyed by an ' active ' process, that is to say, one in which energy is uti1ised.l This may sometimes cause the ionic concentrations in the xylem sap to exceed that in the outer medium by a factor of IOO or more.2 Active transfer depends on energy released by respiration. This has been amply demonstrated by many types of study, for example, investigations of the effects of oxygen tension and the supply of respirable carbohydrates or the action of respiratory inhibitors. The nature of the mechanism, however, still remains ~n k n o w n .~ At one time it was widely held that the electron transfer in respiration directly mediated the accumulation of ions, but it is now considered more probable that the connexion between respiration and accumulation is considerably less direct and is possibly linked with protein synthesis4 Clearly, however, the process depends on ions being restrained by some product of metabolism in consequence of which they are moved against electrochemical gradients. Whether anions or cations are subject to this process, the other ion moving passively, or whether ions of both signs are transferr...