The accelerated effect of recombinant human bone morphogenetic protein 2 delivered by β-tricalcium phosphate on tendon-to-bone repair process in rabbit models

Hirakawa, Yoshihiro; Manaka, Tomoya; Orita, Kumi; Ito, Yoichi M.; Ichikawa, Koichi; Nakamura, Hiroaki

doi:10.1016/j.jse.2017.11.025

Cited by 15 publications

(10 citation statements)

References 38 publications

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“…In order to obtain more new bone formation, and thus tendon healing at the bone interface can be improved, researchers have attempted to use osteo-biomaterials, such as calcium phosphate ceramics and bone morphogenetic proteins. 25,45,46 The in vivo results gained with the osteo-biomaterial were desirable. Previous studies reported that GO exhibits the ability to allow for the migration and growth of osteoblasts and mesenchymal stem cells and had a greater osteoconductivity ability compared with traditional calcium phosphate ceramics.…”

Section: Discussionmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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Background These normal entheses are not reestablished after repair despite significant advances in surgical techniques. There is a significant need to develop integrative biomaterials, facilitating functional tendon-to-bone integration. Materials and methods We fabricated a highly interconnective graphene oxide-doped electrospun poly(lactide-co-glycolide acid) (GO-PLGA) nanofibrous membrane by electrospinning technique and evaluated them using in vitro cell assays. Then, we established rabbit models, the PLGA and GO-PLGA nanofibrous membranes were used to augment the rotator cuff repairs. The animals were killed postoperatively, which was followed by micro-computed tomography, histological and biomechanical evaluation. Results GO was easily mixed into PLGA filament without changing the three dimensional microstructure. An in vitro evaluation demonstrated that the PLGA membranes incorporated with GO accelerated the proliferation of BMSCs and furthered the Osteogenic differentiation of BMSCs. In addition, an in vivo assessment further revealed that the local application of GO-PLGA membrane to the gap between the tendon and the bone in a rabbit model promoted the healing enthesis, increased new bone and cartilage generation, and improved collagen arrangement and biomechanical properties in comparison with repair with PLGA only. Conclusion The electrospun GO-PLGA fibrous membrane provides an effective approach for the regeneration of tendon to bone enthesis.

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Section: Discussionmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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“…Several parameters, such as the type of the cells and scaffold phases, determine the appropriate growth factor type and strategy. For instance, growth factor gradients, in type, concentration, and/or releasing time, are required to promote a differential proliferation for seeded cells in interface tendon scaffolds. ,,, Basic fibroblast growth factor (bFGF) (also known as fibroblast growth factor 2 (FGF-2)), growth differentiation factor (GDF), − platelet-derived growth factor (PDGF), , and transforming growth factor-beta 3 (TGF-β3) are commonly used in TL tissue engineering. , When the interfacial regeneration is considered, several factors including transforming growth factor-beta 1 (TGF-β1), TGF-β3, different bone morphogenetic proteins (BMPs; e.g., BMP-12, BMP-7, BMP-2), ,− PDGF-BB, FGF-2, and F2A (peptide mimetic of FGF-2) have been employed to enhance insertional healing and improve the interfacial strength both in vitro and in animal models. Thus, the optimization of cell culture medium supplementation has attracted much interest; however, there are limited reports on growth factor signal using biodegradable scaffolds.…”

Section: Signaling Strategies In Biodegradable Scaffoldsmentioning

confidence: 99%

“…Although nanofiber alignment had no effect on gene expression, the immobilized PDGF improved the proliferation and tenogenic differentiation of ADSCs. Hirakawa et al showed that the tendon-to-bone regeneration process in a rabbit model was accelerated using BMP-2 delivered by β-tricalcium phosphate (β-TCP). In another study, bFGF was loaded on electrospun PLGA fibrous scaffolds to enhance the repair of rotator cuff tears (RCTs) .…”

Section: Signaling Strategies In Biodegradable Scaffoldsmentioning

confidence: 99%

Biofabrication and Signaling Strategies for Tendon/Ligament Interfacial Tissue Engineering

Heidari

Ruan

De‐Juan‐Pardo

et al. 2021

ACS Biomater. Sci. Eng.

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Tendons and ligaments (TL) have poor healing capability, and for serious injuries like tears or ruptures, surgical intervention employing autografts or allografts is usually required. Current tissue replacements are nonideal and can lead to future problems such as high retear rates, poor tissue integration, or heterotopic ossification. Alternatively, tissue engineering strategies are being pursued using biodegradable scaffolds. As tendons connect muscle and bone and ligaments attach bones, the interface of TL with other tissues represent complex structures, and this intricacy must be considered in tissue engineered approaches. In this paper, we review recent biofabrication and signaling strategies for biodegradable polymeric scaffolds for TL interfacial tissue engineering. First, we discuss biodegradable polymeric scaffolds based on the fabrication techniques as well as the target tissue application. Next, we consider the effect of signaling factors, including cell culture, growth factors, and biophysical stimulation. Then, we discuss human clinical studies on TL tissue healing using commercial synthetic scaffolds that have occurred over the past decade. Finally, we highlight the challenges and future directions for biodegradable scaffolds in the field of TL and interface tissue engineering.

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“…Their results demonstrated significantly greater load to failure (P = 0.037) and enthesis maturity score (P = 0.010) compared with control at 4 weeks but not at 2 and 8 weeks, potentially suggesting an accelerated healing process without long-term structural benefit. 28 Manipulation of TGF-b signaling and expression has also been of interest. TGF-b1 is endogenously expressed at high levels during adult scar formation but at low levels during fetal wound healing, whereas TGF-b3 exhibits the opposite expression pattern.…”

Section: Growth Factorsmentioning

confidence: 99%

Augmentation of Rotator Cuff Healing With Orthobiologics

Kovacevic

Suriani

Levine

et al. 2021

J Am Acad Orthop Surg

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The limited regenerative capacity of the tendon-bone enthesis after surgical repair poses a significant challenge to achieving desired clinical outcomes. Biologic augmentation of the repair site has the potential to enhance the biomechanical and histological integrity of the enthesis, leading to lower retear rates and greater patient satisfaction. Platelet-rich plasma, stem cells and bone marrow aspirate concentrate, growth factors, biodegradable or biomimetic scaffolds, and amniotic products have been investigated in preclinical and, in some cases, clinical studies aimed at augmenting tendon-bone healing. Although many of these therapies have achieved some degree of success in improving structural, histological, and clinical outcomes after surgical tendon-bone enthesis repair, none have reliably and consistently lead to clinical improvement. High-quality randomized controlled clinical studies are needed to definitively evaluate the efficacy of these biologic therapies and ultimately determine which, if any, are capable of achieving a tendon-bone repair that is structurally noninferior to the native enthesis before injury.T he high rate of reinjury after the surgical repair of rotator cuff tears represents a significant clinical challenge. An estimated 250,000 rotator cuff repairs are performed each year in the United States, 1 and retear rates after surgery have been reported to range from 24 to 94% depending on age, chronicity, tear size, and repair technique, among other factors. 2 Surgical techniques and supplemental therapies that lead to a more robust tendon-tobone reattachment after repair are needed to diminish the psychological and economic burden that results from retears, revision procedures, additional rehabilitation, and missed work.The native tendon-to-bone enthesis morphology is not regenerated after injury and surgical repair. Rather, a structurally and histologically inferior enthesis microarchitecture results (Figure 1). The need to identify biologic agents that have the capacity to regenerate the native tendon-bone enthesis after surgical repair is apparent. Recent preclinical and clinical research has aimed to identify and evaluate the efficacy of these orthobiologics. Therefore, the purpose of this review was to critically analyze preclinical and clinical studies on the use of orthobiologics for tendon-to-bone healing published in

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The accelerated effect of recombinant human bone morphogenetic protein 2 delivered by β-tricalcium phosphate on tendon-to-bone repair process in rabbit models

Cited by 15 publications

References 38 publications

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Biofabrication and Signaling Strategies for Tendon/Ligament Interfacial Tissue Engineering

Augmentation of Rotator Cuff Healing With Orthobiologics

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