The present article deals with the physical properties monitoring of the transmission-hydraulic fluid, and changes of those properties due the operating load of the hydraulic pump in laboratory conditions. Tests of the transmission-hydraulic fluid were performed in laboratory conditions with the simulation of the operating load, so as to simulate the real conditions under which hydraulic circuit of the agricultural tractors operates. The universal transmission-hydraulic fluid was subjected to analysis, where the samples of the fluid were taken sequentially according to the chosen methodology at intervals of 250 and 500 h. The results of the present article include the evaluation of the physical properties of the fluid and the flow efficiency change of the hydraulic pump after 250 and 500 h of operation, while simulating the operation load. Based on a laboratory test, we found that after working for 500 h, the dynamic viscosity of the examined hydraulic fluid at 40°C decreased by 2.92 mPa.s and at 100°C decreased by 0.64 mPa.s. When determining the kinematic viscosity of the hydraulic fluid after working for 500 h, we recorded its decrease, namely, at a temperature of 40°C by a value of 3.37 mm2/s and at a temperature of 100°C, its value decreased by 0.77 mm2/s. In the analysis of the test oil samples, we found that the level of water contamination of the hydraulic fluid decreases with increasing number of hours worked. Specifically, the water concentration in the hydraulic fluid decreased by 0.031%. At the same time, we recorded a slight decrease in the flow efficiency of the hydraulic pump, specifically by a value of 0.03% after 500 h worked. After working 500 h, we found that the range of abrasion particles in the transmission-hydraulic fluid is within the prescribed range, which is determined by the standard D6595-00.