The Accelerating Effect of Calcium and Other Cations on the Conversion of Fibrinogen to Fibrin 1

Ratnoff, Oscar D.; Potts, Albert M.

doi:10.1172/jci102886

Cited by 47 publications

(17 citation statements)

References 9 publications

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“…26 Our team's result further confirmed that calcium doped mesoporous silica spheres possessed superior hemostatic properties compared to pure mesoporous silica spheres. 26 Our team's result further confirmed that calcium doped mesoporous silica spheres possessed superior hemostatic properties compared to pure mesoporous silica spheres.…”

Section: Introductionsupporting

confidence: 63%

Calcium-modified microporous starch with potent hemostatic efficiency and excellent degradability for hemorrhage control

et al. 2015

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Effective hemorrhage control is vital for reducing mortality after major trauma both in civilian life and in the military. In recent years microporous starch (MS) has been used as a hemostatic agent. However, MS has an insufficient hemostatic capacity to stop severe bleeding. To improve its hemostatic performance, in this study calcium-modified microporous starch (CaMS) was firstly developed via oxidization and selfassembly with calcium ions (Ca 2+ ) on MS, and the hemostasis efficiency and degradation behaviour were evaluated. The results showed that the carboxyl groups and Ca 2+ had been modified successfully onto MS. MS and CaMS both initiated the hemostatic response by rapid absorption and swelling due to their porous structure and high surface area. It is noteworthy that CaMS activated an intrinsic pathway of coagulation cascade and induced platelet adhesion because of the modified Ca 2+ and carboxyl groups.The synergistic effects of the chemical activation mechanism and physical absorption mechanism resulted in a dramatic improvement in the hemostatic capacity of CaMS, and thus achieved an effective hemorrhage control in rabbit liver and femoral artery injuries. Additionally, the degradation of CaMS was improved greatly by the modification. In conclusion, CaMS effectively improved hemostatic performance and degradability. CaMS is a promising candidate for designing hemostatic agents in more extensive clinical applications.

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Section: Introductionsupporting

confidence: 63%

Calcium-modified microporous starch with potent hemostatic efficiency and excellent degradability for hemorrhage control

et al. 2015

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“…By contrast, magnesium ions retarded the rates of polymerization of fibrin monomer to degrees resembling those induced by equimolar additions of NaCl. Since addition of MgCI, changed ionic strength to a greater degree than addition of NaCl [2], the two were not considered equivalent. However, the inhibitory effect of MgCI, could be ascribed largely to its nonspecific contribution to salt concentration of the medium.…”

Section: The Effect Of Divalent Cations On the Polymerization Of Dissmentioning

confidence: 99%

“…Although the accelerating effect of calcium [ 11 and related divalent cations such as magnesium, strontium, barium, manganese, cobalt, and nickel upon fibrin clot formation from fibrinogen by thrombin has been well documented [2], the underlying mechanism is still unclear. The conversion of fibrinogen to fibrin clot is a multistep process [3], and previous studies have attempted to examine the specific steps affected by cations, especially by calcium and magnesium, normally existing in appreciable concentrations in blood.…”

Section: Introductionmentioning

confidence: 99%

Effects of divalent cations on the conversion of fibrinogen to fibrin and fibrin polymerization

1982

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Effects of divalent cations on fibrinogen and its reaction with thrombin were re-examined and correlated to improve definition of mechanisms underlying the acceleration of clot formation by the ions. The rate of release of fibrinopeptides from fibrinogen was not affected by any of the specific ions studied, but increased rates of fibrin monomer polymerization were obtained with all but magnesium ions. Maximal acceleration of monomer polymerization was observed with the divalent ions at concentration of 2.5-5 mM, and no added specific ion effects were observed with much higher levels. The acceleratory ions were found to have a corresponding effect on the solubility of fibrinogen, as judged from acceleration of its precipitation at low temperature. Although magnesium ions had no effect on fibrin monomer polymerization, they did have a low ranking effect on cryoprecipitation. These data confirm that the principal effect of divalent cations in the fibrinogen-fibrin transformation is to accelerate fibrin monomer polymerization, and suggest that the acceleration is associated with effects on the solubility of fibrinogen.

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“…(Concentration gradient of about 0.5,0.5, and 40 mmol/l respectively.) The clot-promoting effect of these ions has been demonstrated by several authors (5, 25,26) and has been related to a decreased solubility of fibrin (9) and to an accelerated fibrin polymerisation (1). This effect has been confirmed in the present study, where a shortening of the reptilase clotting time (RCT) was observed after dialysis.…”

Section: The Dialysis Fluid Used In Haemodialysismentioning

confidence: 97%

Impaired Anticoagulant Effect of Heparin in the Artificial Kidney: An Experimental Study

Björnson

Godal

1976

Scandinavian Journal of Clinical and Laboratory Investigation

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Dialysis of blood and plasma was performed in vitro, in a 'mini-Kill' dialyser as well as in dialysis bags. A marked shortening of the thrombin-clotting time was observed, indicating fall in heparin anticoagulant effect. The concentration of heparin, however, as measured by polybrene titration, was substantially less reduced. Fibrin formation, as evidenced by the ethanol gelation test, occurred more often in the dialysed than in the control plasma. In conclusion, the discrepancy between concentration and anticoagulant effect of heparin could be partly explained by influx from the dialysate of calcium, magnesium, and acetate ions. The fibrin-polymerizing effect of these ions was confirmed by a shortening of the clotting time with Reptilase, a proteolytic enzyme not influenced by thrombin inhibitors such as heparin. In addition, liberation of platelet factor 4 may be responsible for some reduction in antithrombin activity of heparin. No evidence of heparin being dialysed or adhering to the dialysis membrane was found.

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The Accelerating Effect of Calcium and Other Cations on the Conversion of Fibrinogen to Fibrin 1

Cited by 47 publications

References 9 publications

Calcium-modified microporous starch with potent hemostatic efficiency and excellent degradability for hemorrhage control

Calcium-modified microporous starch with potent hemostatic efficiency and excellent degradability for hemorrhage control

Effects of divalent cations on the conversion of fibrinogen to fibrin and fibrin polymerization

Impaired Anticoagulant Effect of Heparin in the Artificial Kidney: An Experimental Study

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