The acoustic analysis provides additional information on building tradition and related indoor practice that includes sound, thus deepening our understanding of architectural heritage. In this paper, the sound field of the Orthodox medieval church Lazarica (Kruševac city, Serbia) is examined. Lazarica is a representative of Morava architectural style, developed in the final period of the Serbian medieval state, when also the chanting art thrived, proving the importance of the aural environment in Serbian churches. The church plan is a combination of a traditional inscribed cross and a triconch. After the in situ measurement of acoustic impulse response using EASERA software, we built a computer model in the acoustic simulation software EASE and calibrated it accordingly. Following the parameters (reverberation time (T30), early decay time (EDT) and speech transmission index (STI)), we examined the acoustic effect of the space occupancy, central dome and the iconostasis. In all the cases, no significant deviation between T30 and EDT parameter was observed, which indicates uniform sound energy decay. Closing the dome with a flat ceiling did not show any significant impact on T30, but it lowered speech intelligibility. The height of iconostasis showed no significant influence on the acoustics of Lazarica church.