The action of bile salts on fluid and glucose movement by rat and hamster jejunum, in Vitro

Abstract

Everted sacs of rat and hamster jejunum were used to measure, simultaneously, fluid and glucose movement in the presence of various concentrations of bile salts. Low concentrations of sodium taurocholate or glycocholate did not affect the rates of fluid absorption or transport by either rat or hamster jejunum. Under these conditions, in some cases, while glucose absorption was unaffected, glucose transport was inhibited. High concentrations of bile salts reduced fluid and glucose absorption and transport by both rat and hamster jejunum. Although the transport of glucose against a concentration gradient is completely inhibited at low concentrations of bile salts with rat jejunum, it still occurs with hamster jejunum even in the presence of high bile salt concentrations. In the absence of glucose i n the incubation medium, the rates of fluid absorption by rat and hamster jejunum were greatly reduced. Under these conditions, fluid transport by rat jejunum was completely abolished, whereas fluid transport by hamster jejunum was greatly reduced, but not completely inhibited. However, addition of bile salts to the glucosefree incubation medium did produce a complete inhibition of fluid absorption and transport by both rat and hamster jejunum. Bile salts, within the concentration range used in this investigation, have no effect upon glucose utilization and lactic acid production by either rat or hamster jejunum. Sodium taurocholate and glycocholate, a t the low Concentration of 1 mM, reduced the ATP levels in both rat and hamster jejunal sacs. This reduction of ATP levels in whole sacs was also observed to occur in the mucosal layer in the presence of low concentrations of bile salts. A greater reduction in tissue ATP levels was observed when the bile salt concentration was increased. The amount of ATP i n unincubated rat and hamster jejunum was reduced by approximately 40% when incubated in bicarbonate saline containing glucose. Tissue ATP levels were also reduced when sacs were incubated in a glucose-free medium, a greater reduction being observed i n rat jejunum. In the absence of glucose, bile salts were capable of decreasing ATP levels i n hamster, but not in rat jejunum. The effects of bile salts on fluid and glucose movement by rat and hamster jejunum were discussed in relation to tissue energy sources for these processes. Also, the possible role played by bile salts i n regulating the transference of various substances across the intestinal wall was discussed.

Alterations of Rat Intestinal Disaccharide Absorption by Bile or Conjugated Bile Acids

“…In an isotonic solution 3 mmol/1 taurocholate had no effect on the jejunal net water movement ("absorption"), whereas 10 mmol/1 caused a decreased water absorption. A similar concentration-dependent effect of taurocholate on the jejunal water absorption was described in the hamster (2). In in vivo-experiments in the rat 5 mmol/1 taurocholate inhibited (18) or did not alter (8) the jejunal water transport.…”

The effects of bile salt and raw bile on the intestinal absorption of micellar fatty acid in the rat in vitro

“…showing that raw bile did not affect the transport of 3-O-methyl-D-glucose suggest that the bile did not alter the permeability of the cell membrane to this compound, nor did it affect the cellular machinery responsible for its active transport. It is worthy of note, however, that there is disagreement on the effects of bile salts and raw bile on the absorption and transport of aqueous solutes (Faust & Wu, 1965;Pope, Parkinson & Olson, 1966;Roy, Dubois & Philippon, 1970).…”

Medikamentöse Gallensteinauflösung bei Cholecystolithiasis

“…Sie ist chologen, d.h. im wesentlichen darauf zuriickzufiihren, dab antiabsorptiv und hydragog wirkende Gallens/iuren in gr6Beren Mengen als normalerweise ins Kolon gelangen und dort die Wasserund Elektrolyt-Resorption hemmen. Chologene Diarrhoen sind dem Arzt nach Ileostomien vertraut; die antiabsorptive und hydragoge Wirkung von Gallens/iuren wurde erstmals im Tierexperiment beschrieben (Faust 1963;Faust, Wu 1965;Forth et al 1963Forth et al , 1964. Auch hier entscheidet AusmaB und Dauer der Beeintrfichtigung darfiber, ob die Therapie fortgesetzt werden kann.…”