The Action of Chlorambucil (CB. 1348) and Busulphan (Myleran) on the Haemopoietic Organs of the Rat

Elson, L. A.; Galton, D. A. G.; Till, Morwenna

doi:10.1111/j.1365-2141.1958.tb06038.x

Cited by 96 publications

(27 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2) during the 4 days following the administration of a mid-lethal dose (100 fig/ mouse) of mustine hydrochloride provides quantitative confirmation of the histological observations of Graef et al [8], Elson et al [7], and Kindred [13] which emphasized the sensitivity of the haematopoietic and lymphoid tissues to treatment with nitrogen mustard. The subse quent regeneration in the cellularity of the femoral bone marrow, to at tain values in the normal range by the 8th day post injection, is preced ed by closely parallel changes in the CFU in the medullary cavity of the femoral diaphysis, indicating that the effects of this drug on the size of the haematopoietic stem cell compartment in the endogenous system are similar to those observed using the exogenous spleen colony assay [15].…”

Section: Discussionsupporting

confidence: 57%

See 1 more Smart Citation

Effects of Mustine Hydrochloride on Mouse Haematopoietic and Lymphoid Tissues

Sharp¹,

Thomas²,

Briscoe³

1973

Chemotherapy

View full text Add to dashboard Cite

During the first 4 days after the intravenous administration of a lethal dose (100 μg) of mustine hydrochloride to mice, there is a marked decrease in the weights of the lymph nodes, thymus and spleen and in the cellularity of the femoral bone marrow. In the subsequent 4 days, the cellularity of the bone marrow increases to values within the normal range while the weights of the lymph nodes, thymus and spleen remain exceedingly small. In this bone marrow regenerating after the injection of mustine hydrochloride, 94% of the recognisable haematopoietic cells are granulocytes at various stages of maturation.

show abstract

Section: Discussionsupporting

confidence: 57%

“…Elson [6] and E lson et al [7] performed comparative studies of the effects of X-irradiation, Busulphan (Myleran) and an aromatic nitrogen mustard derivative CB1348 (Chlorambucil) on the haematopoietic tis sues of the rat. These studies extended up to 4 weeks after treatment, but only qualitative data were obtained for the lymphoid tissues.…”

mentioning

confidence: 99%

Effects of Mustine Hydrochloride on Mouse Haematopoietic and Lymphoid Tissues

Sharp¹,

Thomas²,

Briscoe³

1973

Chemotherapy

View full text Add to dashboard Cite

show abstract

“…The drug busulfan, which has been used for experimental and clinical BM transplantation because of its anti-leukemic and anti-myelopoietic effects [ 10, 16,17,36,37,411, was effective in the depletion of myelopoietic stem cells within the BM of PVG (C-) rats at a concentration of 30 mg/kg b.w. when given 3 days before xenografting, without any evidence of morbidity or mortality.…”

Section: Discussionmentioning

confidence: 99%

“…C5a is quantitatively much more potent as a chemotactic agent than C3a and affects a wider range of cells, including neutrophils, monocytes, basophils, and eosinophils [15]. It has previously been shown that the drug busulfan, which has been used for experimental and clinical bone marrow (BM) transplantation, is an extremely effective anti-leukemic and myeloablative agent, involving little immunosuppressive activity because of its minimal impact on lymphoid cells [16,36,371. Intra-peritoneal injection of busulfan in sub-lethal f:…”

Section: Introductionmentioning

confidence: 99%

Busulfan depletes neutrophils and delays accelerated acute rejection of discordant xenografts in the guinea pig-to-rat model

et al. 2003

View full text Add to dashboard Cite

Complement factor plays a critical role in mediating hyperacute rejection of discordant xenografts. In order to explore the mechanism of discordant xenograft rejection, we investigated kinetics and phenotypes of the cellular infiltrate in xenografts in untreated and leukocyte‐depleted recipients, in relation to graft survivial. Guinea pig cardiac xenografts were heterotopically transplanted to totally C6‐deficient PGV (C‐) rats. Grafts were removed after 0, 6, 12, and 24 h (n= 6). Histological evaluation was performed with hematoxylin and eosin (H & E) and immunoperoxidase staining. The agents fucoidin and busulfan were applied to delay xenograft rejection further. Within 6 h, minimal perjection further. Within 6 h, minimal perivascular edema with isolated infiltrating CD11b/c‐ and ED1‐positive cells were found. An intense infiltration of CD11b/c‐ and EDI‐positive cells with intestitial hemorrhage was present after 24 h, though with little CD161 and CD3 cell infiltration. Inhibition of cell adhesion by fucoidin did not prolong xenograft survival (34 ± 15 h, n= 4, P <0.47), but the depletion of granuloecytes by injection of busulfan did prolong survival of the discordant xenograts, to 62±22 h (n 7, P ± 0.0039). These reults demonstrate a significant effect of specific depletion of granulocytes and macrophages by busulfan therapy on guinea pig cardiac xenograft survival in PVG (C‐) rats, suggesting the participation of these infiltrating cells in the xenoreractive rejection process.

show abstract

“…The accumulation is not restricted to guineapigs. It has been noted in the bone marrow of irradiated mice (6) and in rats recovering from the effects of Busulphan (10).…”

mentioning

confidence: 99%