The Action of Cholecystokinin and Related Peptides on Guinea Pig Small Intestine

Yau, William M.; Makhlouf, G.M.; Edwards, Leslie E.; Farrar, John T.

doi:10.1139/y74-041

Cited by 44 publications

(24 citation statements)

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“…The administration of CCK-OP in 20-fold higher dose than that of cerulein, which is considered to be of similar potency (Romań ski, 2004), evoked, however, a stronger effect on the incidence of the MR as compared with the cerulein effect. Yau et al (1974) suggested that CCK-OP and cerulein are equipotent, while Ruckebusch and Soldani (1985) found that a 10-fold lower cerulein dose than CCK-OP evoked an even stronger gallbladder contractile response. These doses were similar to those used in the present study.…”

Section: Discussionmentioning

confidence: 99%

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Romanski

2009

Animal

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Cholecystokinin (CCK) can exert multiple actions on intestinal motility but its effect on the small-intestinal 'minute rhythm' (MR) is virtually unknown. Therefore, the electrical activity from the abomasal antrum, duodenal bulb, duodenum, jejunum and ileum was continuously recorded in six sheep before, during and after slow intravenous administration, of three doses each, of cholecystokinin-octapeptide (CCK-OP) and cerulein. In four of these sheep, two additional electrodes and the strain gauge force transducer were also inserted in the duodenum. Chronic experiments were performed in the fasted and non-fasted animals and saline or CCK peptides were injected during phases 1, 2a or 2b of the duodenal migrating myoelectric complex (MMC). The administration of both CCK peptides in various doses evoked an inhibitory effect mostly in the duodenal bulb, except for the lowest dose of cerulein. The effects of 20 times greater doses of CCK-OP than that of cerulein were more pronounced. The introduction of both CCK peptides during phase 1 of the MMC produced no marked or significant response. In non-fasted animals, the effects of both hormonal peptides, given during phase 2b of the MMC, were often stronger than those given during phase 2a, while in fasted animals the effects of CCK peptides, administered in the course of phases 2a and 2b of the MMC, were similar. Both higher doses of CCK peptides increased the number of spike bursts within the given MR pattern in the duodenum and decreased the incidence of MR mostly in the duodenal bulb. The inhibitory effects of both CCK peptides on the bulbar MR exhibited a dose-response character, though the lowest dose often evoked the slight stimulatory response. It is concluded that CCK principally exerts an inhibitory effect upon the MR in the duodenal bulb and modifies the MR in the duodenum by increasing the spike burst number in a given MR pattern. Both these actions of CCK peptides seem to be physiological. There is a positive relationship between the intensity of the refractory period and the demonstrated effect of CCK in the duodenum.

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Section: Discussionmentioning

confidence: 99%

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Romanski

2009

Animal

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“…In vitro studies have shown that this CCK effect results from direct hormonal action on gallbladder musele (4,5). It has been proposed that this action of CCK on the gallbladder is physiological (6).…”

Section: Introductionmentioning

confidence: 99%

Effect of cholecystokinin and the octapeptide of cholecystokinin on the feline sphincter of Oddi and gallbladder. Mechanisms of action.

Behar¹,

Biancani²

1980

J. Clin. Invest.

158

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A B S T R A C T WVe studied the effect of cholecystokinin (CCK) and the octapeptide of cholecystokinin (OP-CCK) on the feline gallbladder and sphincter of Oddi. Both CCK caused a dose-dependent gallbladder contraction and sphincter of Oddi relaxation. The halfmaximal responses of the sphincter of Oddi were 6 ng/kg for OP-CCK and 0.15 Ivy-dog U/kg for CCK, which were lower than those ofthe gallbladder wvith 28 ng/kg and 0.32 Ivy-dog U/kg, respectively. The effect of OP-CCK on the gallbladder was partially blocked by tetrodotoxin (P < 0.02), hexamethonium alone (P < 0.05), or a combination of hexamethonium and atropine (P < 0.01). The gallbladder response to CCK wvas not blocked by either atropine alone (P < 0.60) or adrenergic anitagonists (P > 0.40). The sphincter of Oddi response to OP-CCK was blocked by tetrodotoxin (P < 0.001) but it was not blocked by cholinergic (P < 0.20) or adrenergic antagonists (P < 0.60). After comiplete denervation with tetrodotoxin, OP-CCK caused sphincter of Oddi contraction. These findings indicate that there are two excitatory receptors for CCK in the gallbladder, one at the cholinergic neurons and the other at the level of the gallbladder muscle. There are also tvvo receptors for CCK in the sphincter of Oddi, one that is inhibitory, and present at the noncholinergic, nonadrenergic neurons, and the other, excitatory, at the circular muscle.Address reprint requtests to Jose Behar, M. D., Rhode Island Hospital, Providence, R.

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“…On the basis of our data and that published in the literature we propose the following mechanisms. The excitatory respohses of the gall-bladder, small intestine, and Heidenhain pouch to CCK-7 and CCK were suppressed by atropine and ganglion blockers and were mediated, therefore, through release of acetylcholine, as has been demonstrated for isolated gallbladder and gastrointestinal smooth muscle (Yau & Farrar, 1972;Fisher, DiMarino & Cohen, 1973;Vizi, Bertaccini, Impicciatore & Knoll, 1973). The inhibitory effects of CCK-7 and CCK on the motility and spike discharges in stomach and duodenum (Doringer et al, 1975;Oigaard et al, 1975) are mediated not by adrenergic transmission but probably by other hormonal factors, e.g secretin, somatostatin or VIP, and this is supported by the following observations:…”

Section: Effects Of Various Drugs On Cck Responsesmentioning

confidence: 69%

Effect of desamino‐cholecystokinin‐octapeptide (CCK‐7) on the intraluminal pressure and myoelectrical activity of the gall‐bladder, stomach, and small intestine in conscious dogs

Milenov

Nieber²,

Niedrich³

et al. 1983

British J Pharmacology

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Desamino‐cholecystokinin‐octapeptide (CCK‐7) increased the gall‐bladder pressure and decreased the gastric pressure following i.v. infusion in conscious dogs. In small intestine and Heidenhain pouch CCK‐7 exerted an initial excitatory effect followed by an inhibitory effect on intraluminal pressure and peristalsis. In all organs the effect of CCK‐7 on pressure was correlated with a change of spike discharge. CCK‐7 is more potent on gastrointestinal motor activity than a Boots preparation of pancreozymin. The results suggest that CCK‐7 at doses within the physiological range could be involved in the regulation of gastrointestinal motility.

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The Action of Cholecystokinin and Related Peptides on Guinea Pig Small Intestine

Cited by 44 publications

References 0 publications

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Cholecystokinin-dependent selective inhibitory effect on ‘minute rhythm’ in the ovine small intestine

Effect of cholecystokinin and the octapeptide of cholecystokinin on the feline sphincter of Oddi and gallbladder. Mechanisms of action.

Effect of desamino‐cholecystokinin‐octapeptide (CCK‐7) on the intraluminal pressure and myoelectrical activity of the gall‐bladder, stomach, and small intestine in conscious dogs

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