The Activity and Organophosphate Inhibition of Cholinesterases From Susceptible and Resistant Ticks (Acari)

Lee, R. M.; Batham, P.

doi:10.1111/j.1570-7458.1966.tb00974.x

Cited by 67 publications

(27 citation statements)

References 7 publications

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“…The other is relatively insensitive and the difference between the systems lies in the nature of this component, which exhibits relatively greater insensitivity and contributes more to total activity in the former than it does in the latter system. Features similar to these have been described previously for the Ridgelands (Lee and Batham 1966) and Biarra enzymes Wharton and Roulston 1970). In Fig.…”

Section: Kinetics Of Inhibition Of Acetylcholinesterase Activity In Vsupporting

confidence: 85%

Characterization of the Organophosphoms-resistant Mt Alford, Gracemere and Silkwood Strains of the Cattle Tick, Boophilus microplus

Schnitzerling¹,

Schuntner²,

Roulston³

et al. 1974

Aust. Jnl. Of Bio. Sci.

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Dosage-mortality tests on larval ticks have indicated that the Mt Alford and Gracemere strains of B. micro pius differ from the Biarra and Ridgelands strains respectively, not only as previously reported by exhibiting markedly greater resistance to both chlorpyrifos and diazinon, but also by exhibiting moderately greater resistance in the Mt Alford strain to bromophos-ethyl and dioxathion and in the Gracemere strain to carbophenothion and coumaphos. Their responses to other chemicals were similar to those of the Biarra and Ridgelands strains respectively.Tests of acetylcholinesterase (AChE) activity and kinetic studies of inhibition of AChE in Mt Alford and Gracemere larvae indicated the presence of enzymes with relatively insensitive components similar to those of Biarra and Ridgelands AChE respectively.The penetration of [I4C]chlorpyrifos was similar in Mt Alford, Gracemere, Biarra and Ridgelands larvae, but of the chlorpyrifos metabolized considerably less was recovered as the oxygen analogue in Mt Alford and Gracemere larvae. This difference appeared to be due to greater hydrolytic activity. It was concluded that the greater resistance of these strains is due to enhanced detoxication.The previously described Mackay strain, although maintaining its pattern of resistance, was shown to have changed to one now exhibiting a reduced capacity for detoxication and a Ridgelands type of AChE. The Silkwood strain found nearly three years later was shown to be similar to the changed Mackay strain.

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Section: Kinetics Of Inhibition Of Acetylcholinesterase Activity In Vsupporting

confidence: 85%

Characterization of the Organophosphoms-resistant Mt Alford, Gracemere and Silkwood Strains of the Cattle Tick, Boophilus microplus

Schnitzerling¹,

Schuntner²,

Roulston³

et al. 1974

Aust. Jnl. Of Bio. Sci.

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“…Altered AChE has also been reported in Australian B. microplus tick strains (Lee & Batham, 1966;Roulston ei al., 1968). Wright & Ahrens (1988) reported that an insensitive AChE is the major mechanism displayed by Mexican strains that develop resistance to coumaphos.…”

Section: Introductionmentioning

confidence: 88%

Detection of esterase activity in susceptible and organophosphate resistant strains of the cattle tickBoophilus microplus(Acari: Ixodidae)

Rosario-Cruz

Miranda-Miranda

Garcia-Vasquez

et al. 1997

Bull. Entomol. Res.

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Two organophosphate (OP) resistant strains of the cattle tick Boophilus microplus (Canestrini) from Mexico and Costa Rica were used to analyse the presence of esterase activity associated with resistance. The concentrations of six major proteins in both resistant strains were increased compared to the susceptible Morelos strain, both when stained with Coomassie Brilliant Blue after SDS-PAGE, and when analysed for esterase activity by the hydrolysis of naphthyl acetate esters. Esterases were named A or B in relation to the substrate preference for alpha or beta naphthyl acetate and numbered according to their position on the SDS-PAGE. The molecular weights of these proteins were: 125, 115, 108, 77, 43 and 67 Kd for Est-Bl, Est-B2, Est-B3, Est-B4, Est-B5 and Est-A respectively. Est-B3 showed cholinesterase (ChE) activity. This study strengthens the hypothesis that the mechanism associated with OP resistance found in many other insects includes an increase of esterase activity, probably as a result of gene amplification. The genes encoding these enzymes could be potentially used as molecular markers to detect resistance in the cattle tick B. microplus using a DNA probe.

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“…Emergence and increasing incidence of tick resistance to OP exposure in Mexico and other countries threaten the US cattle industry and complicate efforts by the CFTEP to maintain an effective entry barrier and to eradicate outbreak infestations [7]. The major mechanism of resistance to OP acaricides in ticks was biochemically characterized as AChE insensitivity to OP inhibition in extracts of tick synganglia [8][9][10] and resistance may also have a metabolic detoxification component [11].…”

Section: Ticksmentioning

confidence: 99%

Acetylcholinesterases of blood-feeding flies and ticks

Temeyer

Tuckow

Brake

et al. 2013

Chemico-Biological Interactions

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The Activity and Organophosphate Inhibition of Cholinesterases From Susceptible and Resistant Ticks (Acari)

Cited by 67 publications

References 7 publications

Characterization of the Organophosphoms-resistant Mt Alford, Gracemere and Silkwood Strains of the Cattle Tick, Boophilus microplus

Characterization of the Organophosphoms-resistant Mt Alford, Gracemere and Silkwood Strains of the Cattle Tick, Boophilus microplus

Detection of esterase activity in susceptible and organophosphate resistant strains of the cattle tickBoophilus microplus(Acari: Ixodidae)

Acetylcholinesterases of blood-feeding flies and ticks

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