The Adaptation of Rhodopseudomonas sphaeroides f. sp. denitrificans for Growth Under Denitrifying Conditions

Michalski, Wojciech P.; Nicholas, D. J. D.

doi:10.1099/00221287-130-1-155

Cited by 10 publications

(16 citation statements)

References 12 publications

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“…In common with other phototrophic bacteria, R. sphaeroides subsp. denitrificans grown in light under anaerobic conditions synthesizes both bacteriochlorophyll a and carotenoids and derives ATP for growth from photosynthetic electron transport (20,24,26 further 12 h of growth ( Fig. 3a and b).…”

Section: Resultsmentioning

confidence: 99%

“…Bacteriochlorophyll a was extracted in acetone-methanol (7:2 [vol/vol]) from washed cell suspensions and determined by using an extinction coefficient value of 76 mM cm-' at 772 nm (24). The pigment was also purified from acetonemethanol extracts by powdered-sugar column chromatography.…”

Section: Methodsmentioning

confidence: 99%

“…denitrificans IL106 was kindly supplied by T. Satoh, Department of Biology, Tokyo Metropolitan University, Tokyo, Japan. It was grown photoheterotrophically in a mineral salt medium as described previously (24). Analysis of pigments.…”

Section: Methodsmentioning

confidence: 99%

“…1) with butan-1-ol-acetic acid-H20 ( Electrophoresis. Details of sodium dodecyl sulfate (SDS)-polyacrylamide slab gel electrophoresis and sample preparations were as previously described (24). Gradient gel electrophoresis was run on Pharmacia PAA 4/30 gels.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to these two distinct growth regimes, it can also generate ATP by nitrate respiration (19,24,32). The presence of nitrate in a photosynthetically grown bacterial culture enhanced the synthesis of nitrate, nitrite, and nitrous oxide reductases (22,24) but markedly depressed the synthesis of carotenoids, bacteriochlorophyll, and soluble cytochromes (23)(24)(25)(26). Denitrifying cells also contain fewer chromatophores, and the number of reaction center complexes is about 60% of that of nondenitrifying cells (26).…”

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confidence: 99%

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Inhibition of bacteriochlorophyll synthesis in Rhodobacter sphaeroides subsp. denitrificans grown in light under denitrifying conditions

Michalski

Nicholas

1987

J Bacteriol

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The inclusion of nitrate or nitrite in cultures of Rhodobacter spaeroides subsp. denitrificans grown heterotrophically in light depressed the formation of bacteriochlorophyll a. The pigment biosynthesis was inhibited at the stage of the reduction of chlorophyllide (chlorin) to bacteriochlorophyllide (tetrahydroporphyrin) since 3-hydroxyethylchlorophyllide a accumulated in the culture medium. The addition of exogenous 5-aminolevulinic acid to these cultures resulted in a complete restoration of bacteriochlorophyll synthesis accompanied by the accumulation of 3-vinylbacteriopheophorbide. This indicates that under these conditions bacteriochlorophyll was formed via an alternative route, in which the reduction of chlorins to tetrahydroporphyrins precedes modifications of the C-3 side chain. The multiple forms of 5-aminolevulinic acid synthase were purified from cells grown with and without nitrate. Antibodies against these proteins were raised in rabbits and used in enzyme-linked immunosorbent assays for various forms of 5-aminolevulinic acid synthase. In denitrifying cells, the amount and activity of fraction I of the enzyme was reduced by approximately 40 and 30%, respectively. Partly active enzymes from both types of cells were activated by cystine trisulfide.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Inhibition of bacteriochlorophyll synthesis in Rhodobacter sphaeroides subsp. denitrificans grown in light under denitrifying conditions

Michalski

Nicholas

1987

J Bacteriol

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Isolation and characterization of some chemoautotrophic Chromatiaceae

Kämpf

Pfennig

1986

J. Basic Microbiol.

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Isolation and characterization of some chemoautotrophic Chromatiaceae CHAHLUTTE: KAMPF and N. PFENNIG (Ileceived M a y 26, 1986) Three strains of facultatively chemoautotrophic Chromatiaceae were isolated from natural habitats under chemotrophic conditions and identified as Chromatium gracile (strains 23-3 and 25-1) and T h i o c~p~a roseopersicina (strain LABOE). The strains utilized hydrogen and reduced sulfur compounds for photo-and chemolithosutotrophic growth. Ammonium or dinitrogen were utilized as nitrogcn sources for photo-and chemotrophic growth. With most of the organic carbon compounds examined chemoheterotrophic growth was dependend on thc presence of a reduced sulfur compound. The newly isolated strains grew chemoorganotrophically with fumarate, fructose as well as with some short-chain fatty acids and aliphatjc alcohols. The determination of the respiratory activity of Chromatium gracile 25-1 revealed that the utilization of butyrate for chemotrophic growth was not constitutive. The maximal respiratory activity of phototrophically grown Chromatium gracile 25-1 was 2 times higher than that of other facultative cliemotrophic Chromatiaceae. The respiratory activity of the obligately phototrophic Thiocapsa pfennigii was similar to that of phototrophically grown facultatively chemotrophic Chromatiaceae. 33'

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Regulation of nitrogen fixation by nitrite and glutamine in Derxia gummosa

Wang¹

1986

FEMS Microbiology Letters

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Nitrogenase activity of cells of Derxia gummosa (30 h growth in cultures without combined nitrogen) was not inhibited on adding nitrate. However, on adding either azaserine or methionine sulfoximine (MSX) with nitrate to these cells, nitrogenase (C2H2 reduction) was inhibited because nitrite accumulated in the reaction mixtures. Nitrite inhibition of the in vivo C2H2 reduction had a Ki value of 16 μM. Both ammonia and glutamine inhibited N2 fixation (C2H2 reduction) in intact cells and in those treated with toluene. This inhibition by ammonia was relieved by methionine sulfoximine but not by glutamine. Azaserine enhanced the inhibition of nitrogenase produced by either ammonia or glutamine, since these treatments resulted in an accumulation of glutamine.

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The Adaptation of Rhodopseudomonas sphaeroides f. sp. denitrificans for Growth Under Denitrifying Conditions

Cited by 10 publications

References 12 publications

Inhibition of bacteriochlorophyll synthesis in Rhodobacter sphaeroides subsp. denitrificans grown in light under denitrifying conditions

Inhibition of bacteriochlorophyll synthesis in Rhodobacter sphaeroides subsp. denitrificans grown in light under denitrifying conditions

Isolation and characterization of some chemoautotrophic Chromatiaceae

Regulation of nitrogen fixation by nitrite and glutamine in Derxia gummosa

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