The adjuvanticity of Ganoderma lucidum polysaccharide for Newcastle disease vaccine

Zhang, Ping; Ding, Ronglong; Jiang, Shanxiang; Ji, Liwei; Pan, Mingming; Liu, Li; Zhang, Wei; Gao, Xiaona; Huang, Wenjuan; Zhang, Guanjun; Lin, Peng; Jiang, Hui

doi:10.1016/j.ijbiomac.2014.01.067

Cited by 20 publications

(12 citation statements)

References 31 publications

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“…Hsu et al also has demonstrated that an extract of G. lucidum polysaccharides could induce IL-1 expression via TLR4-modulated protein kinase signaling pathways in macrophages (47). Zhang et al also has reported that the G. lucidum polysaccharide acts as an adjuvant for chicken Newcastle disease vaccine (48). Here, we found that PS-G could effectively induce EV-A71-specific immune responses via the intranasal route.…”

Section: Discussionsupporting

confidence: 64%

A Polysaccharide Purified From Ganoderma lucidum Acts as a Potent Mucosal Adjuvant That Promotes Protective Immunity Against the Lethal Challenge With Enterovirus A71

et al. 2020

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Enterovirus A71 (EV-A71), the pathogen responsible for the seasonal hand-footand-mouth epidemics, can cause significant mortality in infants and young children. The vaccine against EV-A71 could potentially prevent virus-induced neurological complications and mortalities occurring due to the high risk of poliomyelitis-like paralysis and fatal encephalitis. It is known that polysaccharide purified from Ganoderma lucidum (PS-G) can effectively modulate immune function. Here, we used PS-G as an adjuvant with the EV-A71 mucosal vaccine and studied its effects. Our data showed that PS-G-adjuvanted EV-A71 generated significantly better IgA and IgG in the serum, saliva, nasal wash, bronchoalveolar lavage fluid (BALF), and feces. More importantly, these antibodies could neutralize the infectivity of EV-A71 (C2 genotype) and cross-neutralize the B4, B5, and C4 genotypes of EV-A71. In addition, more EV-A71-specific IgA-and IgG-secreting cells were observed with the used of a combination of EV-A71 and PS-G. Furthermore, T-cell proliferative responses and IFN-γ and IL-17 secretions levels were notably increased in splenocytes when the EV-A71 vaccine contained PS-G. We also found that levels of IFN-γ and IL-17 released in Peyer's patch cells were significantly increased in EV-A71, after it was combined with PS-G. We further demonstrated that both CD4 + and CD8 + T cells were able to generate IFN-γ and IL-17 in the spleen. An easy-accessed model of hybrid hSCARB2 + / + /stat-1 − / − mice was used for EV-A71 infection and pathogenesis. We infected the mouse model with EV−A71, which was

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Section: Discussionsupporting

confidence: 64%

A Polysaccharide Purified From Ganoderma lucidum Acts as a Potent Mucosal Adjuvant That Promotes Protective Immunity Against the Lethal Challenge With Enterovirus A71

et al. 2020

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“…Immunologic adjuvant plays an important role in prevention of infectious disease with different types of vaccine, such as aluminum and oil adjuvant [1], but these chemical adjuvants commonly cause side effects, such as strong local stimulation and carcinogenesis [6,7]. Some chemical adjuvants failed to enhance the immunogenicity of weak antigen vaccine [8].…”

Section: Introductionmentioning

confidence: 99%

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Yang

Zhang

Xu³

et al. 2019

Bioscience Reports

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The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated in chickens. The results showed that in a certain range of concentrations, PTFP treatment can dose-dependently enhance lymphocyte proliferation. Then, 280 14-days-old chickens were randomly divided into seven groups, and vaccinated with ND vaccine except blank control (BC) group. At the first vaccination, chickens were orally administrated with PTFP at concentration ranging from 0 to 50 mg/kg once a day for 3 successive days, and the BC group was treated with physiological saline. The lymphocyte proliferation rate, serum antibody titer, and levels of interferon-γ (IFN-γ) were respectively measured on 7, 14, 21, and 28 days after the first vaccination. The results showed that PTFP at the suitable doses could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IFN-γ concentrations. Taken together, these data indicated that PTFP could improve the immune efficacy against ND vaccine in chickens, and could be as the candidate of a new-type immune adjuvant.

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“…Thus, the active sites of PGPS tc and PGPS 60c were selected and subjected to further experiments by synthetic analysis. Zhang et al have demonstrated that in vitro Ganoderma lucidum polysaccharide (GLP) could significantly enhance lymphocytes proliferation singly or synergistically with ConA [ 19 ]. Liu et al have reported that Atractylodes macrocephala polysaccharide (AMP) and its selenium-modified products at certain concentrations could significantly promote lymphocytes proliferation and enhance cellular immunity [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro

et al. 2017

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The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4+ and CD8+ T cells were performed. Four different concentrations of PGPSs (PGPStc, PGPS60c, PGPS80c, and PGPStp) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPStc groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPStc and PGPS60c were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4+ and CD8+ T cells. At most time points, PGPStc could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments.

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The adjuvanticity of Ganoderma lucidum polysaccharide for Newcastle disease vaccine

Cited by 20 publications

References 31 publications

A Polysaccharide Purified From Ganoderma lucidum Acts as a Potent Mucosal Adjuvant That Promotes Protective Immunity Against the Lethal Challenge With Enterovirus A71

A Polysaccharide Purified From Ganoderma lucidum Acts as a Potent Mucosal Adjuvant That Promotes Protective Immunity Against the Lethal Challenge With Enterovirus A71

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro

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