The adsorption immobilization of bromelain on chitosan matrices

Olshannikova, Svetlana; Koroleva, Victoria; Holyavka, M. G.; Pankova, S. M.; Belenova, A. S.; Kondratyev, Maxim S.; Samchenko, Alexander A.; Kabanov, Artyom; Artyukhov, V. G.

doi:10.1016/j.jbiotec.2018.06.221

Journal of Biotechnology

2018

DOI: 10.1016/j.jbiotec.2018.06.221

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The adsorption immobilization of bromelain on chitosan matrices

Svetlana Olshannikova

Victoria Koroleva

M. G. Holyavka

et al.

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“…A., KABANOV A. V., KOMAROV V. M., TERENTYEV V. V. The molecular mechanism of adsorption immobilization of inulinase on polymer matrices. Biophysics; v.62(1) , p. [5][6][7][8][9][10][11]2017…”

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Ir-Spectroscopic Study of Immobilization of Selenium Compounds on Biomodified Collagen

Глотова¹,

Galochkina²,

Селеменев³

et al. 2019

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The aim of the work was to research the spectral characteristics of collagen substances bioengineered by using complex collagenolytic proteases preparation obtained before and after the immobilization of selenium compounds in acidic and alkaline media and to comparate the degree of interaction of selenium preparations with collagen matrix upon immobilization and its influence on the conformation of protein molecules. The results of the immobilization of selenium preparations on biomodified collagen by the IR spectroscopy method are presented, analyzed, and discussed. Biomodified collagen was obtained from beef trimming waste (veins and tendons) by sequential peroxide-alkaline and enzymatic hydrolysis with the food collagenase preparation. The following sources of selenium were used as compounds with bioprotective properties for subsequent immobilization on biomodified collagen proteins: 4,4-di[3(5-methyldiprazolil)]selenide (DMDPS) with the content of 0,657g DMDMS in 100 cm3 and sodium selenite. Spectrograms are carried out for products of biomodification of collagen before sorption of compounds of selenium, (at the rate of 1.2 g-6 of selenium on 1 g of collagen) in acid (рН =5) and alkaline (рН =10) environments at a research of influence of compounds of selenium on IR spectrums of products of biomodification of collagen. It was established, that the immobilization takes place by a chemical reaction of selenium preparations with functional groups of the side chains of protein molecules, and its degree varies in the range Na2SeO3 (pH=5) > 4,4-di[3(5-methyldiprazolil)]selenide (DMDPS) > Na2SeO3 (pH=10). It is shown that under the interaction of selenium products with collagen, there is no change in the conformations of its molecules occurred.

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mentioning

confidence: 99%

Ir-Spectroscopic Study of Immobilization of Selenium Compounds on Biomodified Collagen

Глотова¹,

Galochkina²,

Селеменев³

et al. 2019

PTQ

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A Possible Role of Charged Amino-Acid Clusters on the Surface of Cysteine Proteases for Preserving Activity when Binding with Polymers

et al. 2022

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Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry

Mao,

Jiang,

Xiong

et al. 2024

Foods

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This review summarizes the latest progress in enzyme preparation, including enzyme design and modification technology, exploration of new enzyme sources, and application of enzyme preparation in food processing, detection, and preservation. The directed evolution technology improved the stability and catalytic efficiency of enzymes, while enzyme immobilization technology enhanced reusability and industrial applicability. Extremozymes and biomimetic enzymes exhibit excellent performance under harsh conditions. In food processing, enzyme preparation can improve food quality and flavor. In food detection, enzymes combined with immune detection and biosensors realize rapid detection of allergens, pollutants, and pesticide residues. In food preservation, enzymes enhance food quality by extending shelf life and inhibiting microbial growth. In the future, enzyme engineering will be combined with computer-aided design, artificial intelligence, and new material technology to promote intelligent enzyme design and multifunctional enzyme preparation development and help the technological upgrading and sustainable development of the food industry and green chemistry.

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The adsorption immobilization of bromelain on chitosan matrices

Cited by 3 publications

References 0 publications

Ir-Spectroscopic Study of Immobilization of Selenium Compounds on Biomodified Collagen

Ir-Spectroscopic Study of Immobilization of Selenium Compounds on Biomodified Collagen

A Possible Role of Charged Amino-Acid Clusters on the Surface of Cysteine Proteases for Preserving Activity when Binding with Polymers

Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry

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