The advances in brucellosis vaccines

Hou, Huanhuan; Liu, Xiaofeng; Peng, Qisheng

doi:10.1016/j.vaccine.2019.05.084

Cited by 73 publications

(52 citation statements)

References 109 publications

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“…Thus, diagnosis relies mainly on serology. Treatment in farm animals is forbidden in many countries and vaccines are not always protective and safe for human health [5]. Eradication programs strictly follow test and slaughter/culling policy.…”

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

et al. 2019

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Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad (n = 87) and Bahawalpur (n = 94). Presence of antibodies against B. canis and B. abortus/B. melitensis was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate Brucella DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for B. abortus DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with B. canis infection, whereas presence of wounds was found to be associated with B. abortus infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on B. canis and B. abortus in dogs in Pakistan.

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Section: Introductionmentioning

confidence: 99%

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

et al. 2019

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“…Recently, several studies have been carried out to evaluate immunogenicity and protective responses, as well as the application of immunogenic proteins in the diagnosis of brucellosis [14,85]. For instance, the purified rAdk and rSecB of B. abortus have been proposed to be potential candidates for subunit vaccines [86].…”

Section: Serological Proteome Analysis (Serpa)mentioning

confidence: 99%

“…In the last decade, various types of subunit vaccine candidates utilizing proteomic technology have been proposed. However, some problems regarding the levels of protection and safety remain a matter of open discussion [14].…”

Section: Introductionmentioning

confidence: 99%

Proteomics of Brucella: Technologies and Their Applications for Basic Research and Medical Microbiology

et al. 2020

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Brucellosis is a global zoonosis caused by Gram-negative, facultative intracellular bacteria of the genus Brucella (B.). Proteomics has been used to investigate a few B. melitensis and B. abortus strains, but data for other species and biovars are limited. Hence, a comprehensive analysis of proteomes will significantly contribute to understanding the enigmatic biology of brucellae. For direct identification and typing of Brucella, matrix-assisted laser desorption ionization—time of flight mass spectrometry (MALDI—TOF MS) has become a reliable tool for routine diagnosis due to its ease of handling, price and sensitivity highlighting the potential of proteome-based techniques. Proteome analysis will also help to overcome the historic but still notorious Brucella obstacles of infection medicine, the lack of safe and protective vaccines and sensitive serologic diagnostic tools by identifying the most efficient protein antigens. This perspective summarizes past and recent developments in Brucella proteomics with a focus on species identification and serodiagnosis. Future applications of proteomics in these fields are discussed.

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“…So far, vaccination has been successful as a control strategy for preventing exposure to brucellosis in regions where the disease is prevalent (3). Consequently, various studies have demonstrated that live Brucella vaccines such as S19, RB51, S2, and Rev.1 are able to effectively offer immune protection against brucellosis and these are therefore widely used around the world (4).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, various studies have demonstrated that live Brucella vaccines such as S19, RB51, S2, and Rev.1 are able to effectively offer immune protection against brucellosis and these are therefore widely used around the world (4). However, negative implications of these vaccines including diagnostic interference, residual virulence in the host, and pathogenic effects to human health cast doubts to this control strategy (3,(5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Artificially Induced Rough-Mutant Brucella Strain RM57 and Its Parent Strain Brucella melitensis M1981

et al. 2020

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Brucellosis is one of the most common zoonotic epidemics with a serious threat to public health and livestock development in many countries across the world. Vaccination is a key control strategy toward preventing brucellosis in high-prevalence regions. Recently, a rough-type Brucella melitensis mutant strain (RM57) induced from a B. melitensis strain M1981 showed protective effects in guinea pigs indicating that it is a good vaccine candidate. In this study, stress response assays were performed to reveal the mechanisms underlying virulence attenuation of RM57. In addition, a genome-wide transcriptome profile of RM57 was analyzed relative to the parent strain M1981 in order to reveal genetic factors controlling the phenotypes. Our results indicated a similar sensitivity to various stress conditions in RM57 owing to a lack of significant differences from its parent strain. Transcriptome analysis showed that a total of 1,205 genes were differentially expressed between RM57 and M1981 with gene ontology terms revealing that these genes are involved in energy production and conversion, translation, ribosomal structure, and biogenesis. Pathway enrichment analysis revealed that genes involved in oxidative phosphorylation, ribosome, nitrogen metabolism, tyrosine metabolism, and two-component system were significantly affected. As a result of these differences at the molecular level, the function of type IV secretion system in RM57 was found to be affected leading to reduced virulence of the RM57 mutant strain in both macrophage and mice infection models.

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The advances in brucellosis vaccines

Cited by 73 publications

References 109 publications

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

Proteomics of Brucella: Technologies and Their Applications for Basic Research and Medical Microbiology

Comparative Transcriptome Analysis of Artificially Induced Rough-Mutant Brucella Strain RM57 and Its Parent Strain Brucella melitensis M1981

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