The adverse effect of the herbicide atrazine on the reproduction in the intertidal varunid crab Neohelice granulata (Dana, 1851)

Alvarez, Natalia; Avigliano, Luciana; Loughlin, Camila Mac; Rodríguez, Enrique M.

doi:10.1016/j.rsma.2014.12.001

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…When the crab Neohelice granulata was exposed to atrazine during the 3-month pre-reproductive period, during which the ovary is growing out before spawning, a significant ( p < 0.05) delay in ovarian growth was detected, in terms of a lower proportion of vitellogenic oocytes ( Silveyra et al, 2017 , Figure 1A ). The same effect was observed after 1 month of exposure to atrazine during the ovarian re-maturation, i.e., just after the first spawning, once the reproductive period is attained ( Álvarez et al, 2015 ). Correspondingly, during the 3-month exposure mentioned above, a diminished vitellogenin content was found in the ovary of N. granulata , together with a reduced size in both previtellogenic and vitellogenic oocytes ( Silveyra et al, 2017 ).…”

Section: Inhibition Of Ovarian Growthsupporting

confidence: 61%

“…Some evidence of oxidative stress has also been reported in P. clarkii ( Silveyra et al, 2018 ; Yang et al, 2021 ), as well as a decrease in the total hemocyte count ( Yang et al, 2021 ), which represents inhibition of the immune system. On the other hand, a higher incidence of oocyte reabsorption has been reported in N. granulata females exposed during the ovarian re-maturation ( Álvarez et al, 2015 ).…”

Section: Oxidative Stress Histopathological Effects and Depletion Of ...mentioning

confidence: 97%

“…(D) zoea I from hatched from ovigerous females of N. granulata exposed during the entire egg incubation period (H: hydropsy; ASP: atrophied dorsal spine; melanization, atrophy of setae, and atrophied eyes can be also observed). Modified from Álvarez et al (2015) . All figures are reproduced with permission.…”

Section: Inhibition Of Ovarian Growthmentioning

confidence: 99%

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Effects of the Herbicide Atrazine on Crustacean Reproduction. Mini-Review

2022

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Atrazine, one of the most intensively applied herbicides worldwide, is commonly found in several water bodies, affecting the associated fauna. Autochthon crustacean species have been relatively less studied, compared to vertebrate species, particularly concerning reproductive success. In this mini-review, we summarize the relevant information about the effects of atrazine exposure on the main reproductive aspects of crustaceans. One of these effects is related to the inhibition of ovarian growth. In this respect, a diminished vitellogenin content was found in the ovary of crabs exposed to atrazine during the entire period of ovarian growth, in correlation with a reduced oocyte size and a delay of ovarian maturation. Similar results were observed in crayfish. Atrazine was also able to affect the reproductive process, acting as an endocrine disruptor. In this sense, this herbicide was suspected to affect the secretion of some neurohormones involved in the gonadal growth, as well as to alter the circulating levels of steroid hormones which promote the synthesis of vitellogenin for ovarian growth. Moreover, atrazine induced sexual differentiation in juvenile crayfish toward a higher proportion of females, while it produced an increment of males in daphnids. Another aspect affected by this herbicide was the reduction of offspring production, as well as several embryonic abnormalities; genotoxic effects have been also reported in crayfish. Finally, some metabolic imbalances, such as reduction in energy reserves, have been observed in some species, together with oxidative stress and histopathological effects.

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Section: Inhibition Of Ovarian Growthsupporting

confidence: 61%

Section: Oxidative Stress Histopathological Effects and Depletion Of ...mentioning

confidence: 97%

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Effects of the Herbicide Atrazine on Crustacean Reproduction. Mini-Review

2022

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“…Moreover, the results of the 90-days in vivo assays, either made with Roundup Ultramax ® at 0.2 mg/L or with technical glyphosate at 1 mg/L, showed significant oocyte reabsorption. Reactional oocyte atresia has been observed in previous studies made on N. granulata females exposed to several pollutants (39)(40)(41)(42). In this kind of atresia, follicular cells that surround the vitellogenic oocytes invade them to phagocyte the vitellum.…”

Section: Discussionmentioning

confidence: 82%

The Effect of Glyphosate on the Reproduction of Estuarine Crabs: Neohelice granulata as a Study Model

et al. 2021

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This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of Neohelice granulata female crabs, as well as the effects of glyphosate on sperm production in males of the same species. After long-term in vivo assays, made during the 3-month pre-reproductive period of this species, both formulated and technical glyphosate were able to produce a significant incidence of oocyte reabsorption in the ovary, together with a concomitant decreased of vitellogenin content, at concentrations ranging from 0.2 to 1 mg/L. Despite this, after 32-day in vivo assays, glyphosate stimulated oocyte growth, in terms of a higher percentage of vitellogenic oocytes, suggesting that glyphosate could be acting as an endocrine disruptor. In vitro assays made with isolated ovarian pieces showed a decrease of vitellogenin content, in correlation with lower protein synthesis, although some advance in maturation was observed in the histological analysis. In male crabs exposed in vivo to both technical and formulated glyphosate at 1 mg/L, several reproductive imbalances were noted, such as a significant decrease of the sperm count, abnormal spermatophores, and possible disrupting effects of glyphosate on the androgenic gland.

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Interference of an atrazine commercial formulation with the endocrine control of ovarian growth exerted by the eyestalks

Silveyra

Canosa

Zanitti

et al. 2019

Environ Sci Pollut Res

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Atrazine is currently one of the most used herbicides worldwide. We tested the possible effect of the widely used herbicide atrazine on the endocrine control of ovarian growth exerted by the neurohormones secreted at the eyestalk of the estuarine crab Neohelice granulata. For this, both in vivo and in vitro assays were carried out. The in vivo assay comprised the exposure for 1 month to 3 mg/L of a commercial formulation containing 90% of atrazine as active ingredient (Gesaprim 90 WDG®, Syngenta) on three categories of females: intact, ablated of one eyestalk, and ablated of both eyestalks. At the end of the assay, only the intact females showed a significant (p < 0.05) decrease in both content of vitellogenic ovarian proteins and proportion of vitellogenic oocytes, compared to a concurrent control. The results of the in vitro incubation of ovarian pieces with the eventual addition to the incubation medium of eyestalk tissue and/or atrazine at 3 mg/L showed a significant (p < 0.05) decrease in the proportion of vitellogenic oocytes only when atrazine and eyestalk tissue were added. Taken together, these results strongly suggest that the assayed atrazine formulation may act as an endocrine disruptor at the eyestalk level, by altering the normal secretion of some eyestalk hormone, therefore inhibiting ovarian growth.

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The adverse effect of the herbicide atrazine on the reproduction in the intertidal varunid crab Neohelice granulata (Dana, 1851)

Cited by 10 publications

References 30 publications

Effects of the Herbicide Atrazine on Crustacean Reproduction. Mini-Review

Effects of the Herbicide Atrazine on Crustacean Reproduction. Mini-Review

The Effect of Glyphosate on the Reproduction of Estuarine Crabs: Neohelice granulata as a Study Model

Interference of an atrazine commercial formulation with the endocrine control of ovarian growth exerted by the eyestalks

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