The aerobic respiratory chain of Escherichia coli: from genes to supercomplexes

Sousa, Pedro M. F.; Videira, Marco A.M.; Böhn, Andreas; Hood, Brian L.; Conrads, Thomas P.; Goulão, Luís F.; Melo, Ana M.

doi:10.1099/mic.0.056531-0

Cited by 35 publications

(37 citation statements)

References 57 publications

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“…18,35 Therefore, the membrane was an important location for energy transfer. More specifically, the respiratory chain in the bacterial membrane was in charge of electron and energy transfer, in which, the reductive coenzyme of NADH was converted into oxidative coenzyme of NAD + , and O 2 was reduced to H 2 O by gaining the electron, and the energy was released for the subsequent ATP synthesis in the biological system.…”

mentioning

confidence: 99%

“…More specifically, the respiratory chain in the bacterial membrane was in charge of electron and energy transfer, in which, the reductive coenzyme of NADH was converted into oxidative coenzyme of NAD + , and O 2 was reduced to H 2 O by gaining the electron, and the energy was released for the subsequent ATP synthesis in the biological system. 35 The occurrence of membrane silver might pose high risks for the disruption of the respiratory chain, which led to intracellular NADH consumption and ROS accumulation. 36,37 The evaluation for the respiratory chain of E. coli ( Figure 4B) shows that NAD + /NADH ratios are significantly increased by both Cit@ AgNPs (0.57) and Ag + treatments (0.62) comparing to the control (0.48), indicating the imbalance of the key elements in the respiratory chain.…”

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confidence: 99%

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Surface ligand controls silver ion release of nanosilver and its antibacterial activity against <em>Escherichia coli</em>

Long¹,

Hu²,

Yan³

et al. 2017

IJN

126

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Understanding the mechanism of nanosilver-dependent antibacterial activity against microorganisms helps optimize the design and usage of the related nanomaterials. In this study, we prepared four kinds of 10 nm-sized silver nanoparticles (AgNPs) with dictated surface chemistry by capping different ligands, including citrate, mercaptopropionic acid, mercaptohexanoic acid, and mercaptopropionic sulfonic acid. Their surface-dependent chemistry and antibacterial activities were investigated. Owing to the weak bond to surface Ag, short carbon chain, and low silver ion attraction, citrate-coated AgNPs caused the highest silver ion release and the strongest antibacterial activity against Escherichia coli , when compared to the other tested AgNPs. The study on the underlying antibacterial mechanisms indicated that cellular membrane uptake of Ag, NAD + /NADH ratio increase, and intracellular reactive oxygen species (ROS) generation were significantly induced in both AgNP and silver ion exposure groups. The released silver ions from AgNPs inside cells through a Trojan-horse-type mechanism were suggested to interact with respiratory chain proteins on the membrane, interrupt intracellular O 2 reduction, and induce ROS production. The further oxidative damages of lipid peroxidation and membrane breakdown caused the lethal effect on E. coli . Altogether, this study demonstrated that AgNPs exerted antibacterial activity through the release of silver ions and the subsequent induction of intracellular ROS generation by interacting with the cell membrane. The findings are helpful in guiding the controllable synthesis through the regulation of surface coating for medical care purpose.

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confidence: 99%

mentioning

confidence: 99%

Surface ligand controls silver ion release of nanosilver and its antibacterial activity against <em>Escherichia coli</em>

Long¹,

Hu²,

Yan³

et al. 2017

IJN

126

View full text Add to dashboard Cite

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“…NQOs are the electron entry site of the respiratory chain, and they are normally needed for bacterial growth, even in anaerobic conditions . However, an active respiratory chain does not appear to be required in some bacteria, and inactivating NQOs can even stimulate aerobic growth of Z. mobilis .…”

Section: Discussionmentioning

confidence: 99%

Inactivating NADH:quinone oxidoreductases affects the growth and metabolism of Klebsiella pneumoniae

Zhang

Bao

Wei

et al. 2018

Biotech and App Biochem

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NADH:quinone oxidoreductases (NQOs) act as the electron entry sites in bacterial respiration and oxidize intracellular NADH that is essential for the synthesis of numerous molecules. Klebsiella pneumoniae contains three NQOs (NDH-1, NDH-2, and NQR). The effects of inactivating these NQOs, separately and together, on cell metabolism were investigated under different culture conditions. Defective growth was evident in NDH-1-NDH-2 double and NDH-1-NDH-2-NQR triple deficient mutants, which was probably due to damage to the respiratory chain. The results also showed that K. pneumoniae can flexibly use NQOs to maintain normal growth in single NQO-deficient mutants. And more interestingly, under aerobic conditions, inactivating NDH-1 resulted in a high intracellular NADH:NAD ratio, which was proven to be beneficial for 2,3-butanediol production. Compared with the parent strain, 2,3-butanediol production by the NDH-1-deficient mutant was increased by 46% and 62% in glycerol- and glucose-based media, respectively. Thus, our findings provide a practical strategy for metabolic engineering of respiratory chains to promote the biosynthesis of 2,3-butanediol in K. pneumoniae.

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“…17B). Some Gram-negative bacteria, like E. coli, lack complex III, but these organisms have their share in SCs as well, supporting the various respiratory pathways that enable these organisms to grow under varied environmental conditions (Sousa et al, 2011(Sousa et al, , 2012Sousa, Videira, & Melo, 2013;Sousa, Videira, Santos, et al, 2013). Using BNE and CNE combined with kinetic data of wild-type and mutant strains and transcription analyses, these authors identified a number of SCs, including a trimer of SDH, a direct association between the two types of NDH found in E. coli, viz.…”

Section: Bacterial Supercomplexesmentioning

confidence: 95%

Bacterial Electron Transfer Chains Primed by Proteomics

Wessels¹,

Almeida²,

Kartal³

et al. 2016

Advances in Bacterial Electron Transport Systems and Their Regulation

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Electron transport phosphorylation is the central mechanism for most prokaryotic species to harvest energy released in the respiration of their substrates as ATP. Microorganisms have evolved incredible variations on this principle, most of these we perhaps do not know, considering that only a fraction of the microbial richness is known. Besides these variations, microbial species may show substantial versatility in using respiratory systems. In connection herewith, regulatory mechanisms control the expression of these respiratory enzyme systems and their assembly at the translational and posttranslational levels, to optimally accommodate changes in the supply of their energy substrates. Here, we present an overview of methods and techniques from the field of proteomics to explore bacterial electron transfer chains and their regulation at levels ranging from the whole organism down to the Ångstrom scales of protein structures. From the survey of the literature on this subject, it is concluded that proteomics, indeed, has substantially contributed to our comprehending of bacterial respiratory mechanisms, often in elegant combinations with genetic and biochemical approaches. However, we also note that advanced proteomics offers a wealth of opportunities, which have not been exploited at all, or at best underexploited in hypothesis-driving and hypothesis-driven research on bacterial bioenergetics. Examples obtained from the related area of mitochondrial oxidative phosphorylation research, where the application of advanced proteomics is more common, may illustrate these opportunities.

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The aerobic respiratory chain of Escherichia coli: from genes to supercomplexes

Cited by 35 publications

References 57 publications

Surface ligand controls silver ion release of nanosilver and its antibacterial activity against <em>Escherichia coli</em>

Surface ligand controls silver ion release of nanosilver and its antibacterial activity against <em>Escherichia coli</em>

Inactivating NADH:quinone oxidoreductases affects the growth and metabolism of Klebsiella pneumoniae

Bacterial Electron Transfer Chains Primed by Proteomics

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