The AhRR-c.565C>G transversion may increase total antioxidant capacity levels of the seminal plasma in infertile men

Abstract

The Aryl hydrocarbon receptor (AhR)-repressor (AhRR) is a regulator of the AhR pathway, which plays an important role in xenobiotic and reactive oxygen species (ROS) metabolism. Total antioxidant capacity (TAC) is a major factor in semen quality that protects sperm against ROS stress. Malondialdehyde (MDA) is the indicator of lipid peroxidation damage that is occurred due to ROSs. In this study, we determined and compared the MDA and TAC levels of infertile men's semen and blood plasma regarding genotype groups of AhRR-c.565C>G transversion. Semen and blood samples of 123 infertile males were collected from the Fatemeh Zahra IVF Centre, Babol, Iran. TAC and MDA levels of seminal and blood plasma were measured by TBARS and FRAP methods, respectively. Cases were genotyped by the PCR-RFLP method. The frequency of c.565C>G genotypes was determined as CC (34.14%), CG (55.28%) and GG (10.58%). Mean levels of TAC μm/L and MDA nmol/mL in semen plasma of CC, CG and GG groups were (1365.7, 1.28), (1542.8, 1.51) and (1860.2, 0.82), respectively. Also, mean levels of TAC μm/L and MDA nmol/mL in blood plasma samples in CC, CG and GG genotypes were (806.14, 1.168), (727.1, 1.006) and (635.7, 0.83), respectively. Comparison of marker levels between genotypes revealed that the TAC level of semen plasma in the GG genotype was significantly higher than its level in the CC group (p < 0.05). Our findings showed that in seminal plasma of infertile men with the GG genotype of AhRR-c.565C>G transversion, the level of total antioxidant capacity is significantly higher in comparison with the CC genotype. Then, the G allele of AhRR-c.565C>G transversion may have a role in the increase in antioxidant capacity of seminal plasma.

Association of the human aryl hydrocarbon receptor repressor (AhRR)‐c.565C>G transversion with male infertility: A case‐control study from Iran

“…Our results demonstrate that the AhRR codon Pro 185 genotype is associated with both susceptibility and severity of infertility. In addition, the present study suggested that the wild‐type allele of Pro185 is relevant to the incidence of severe infertility …”

“…In addition, there are environmental factors for endocrine disruptors, it can be here mentioned that endocrine disrupters are chemicals that interfere in the body's endocrine system . In addition, the environmental factors impressed the systems of genital, neurological, and human immunity of the human body and the wildlife . A wide range of materials have been recognized as endocrine disrupters including drugs, dioxins and dioxin compounds, such as polychlorinated biphenyls, dichlorodiphenyltrichloroethane, and other pestilences .…”

“…44 In addition, the environmental factors impressed the systems of genital, neurological, and human immunity of the human body and the wildlife. 10 A wide range of materials have been recognized as endocrine disrupters including drugs, dioxins and dioxin compounds, such as polychlorinated biphenyls, dichlorodiphenyltrichloroethane, and other pestilences. 45 These chemicals are detected in many products nowadays including plastic bottles, metal food canisters, detergents, foodstuffs, toys, supplies for pesticides, and pesticides.…”

“…The AhRR protein has been offered including several tumor suppressor genes . The AhR signaling interferes with the poisonous impact of TCDD, containing tumor progress, and influences regulation of cell growth and development …”

Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

“…The MDA in seminal plasma can reflect the degree of peroxidative damage to spermatozoa (Agarwal et al, 2014). In general, our MDA value (1.02 nmol/ml) was slightly higher than the value of 0.94 nmol/ml (infertile) reported by (Colagar et al, 2009) but lower than many other studies (Atig et al, 2017, Josarayi et al, 2017, Collodel et al, 2015. Our seminal plasma MDA values represent all men undergone IVF treatment.…”