The alcohol deprivation effect model for studying relapse behavior: A comparison between rats and mice

Vengeliene, Valentina; Bilbao, Ainhoa; Spanagel, Rainer

doi:10.1016/j.alcohol.2014.03.002

Cited by 162 publications

(143 citation statements)

References 72 publications

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“…This enhanced intake indicates that the HD animals were more sensitive to the four days of interruption to alcohol access, i.e. in the HD animals, this modified intermittent paradigm gave rise to an alcohol deprivation effect [34,35], which is typically not seen when using intermittent access models [22] and to our knowledge only has been reported in one previous study [36]. The alcohol deprivation effect found herein was not as pronounced as that described for some Wistar rats when deprived from alcohol after about two months of continuous access under a 4-bottle free-choice paradigm [35].…”

Section: Alcohol Intake Using Three Consecutive Days Of Intermittent mentioning

confidence: 96%

“…in the HD animals, this modified intermittent paradigm gave rise to an alcohol deprivation effect [34,35], which is typically not seen when using intermittent access models [22] and to our knowledge only has been reported in one previous study [36]. The alcohol deprivation effect found herein was not as pronounced as that described for some Wistar rats when deprived from alcohol after about two months of continuous access under a 4-bottle free-choice paradigm [35]. However, this alcohol access paradigm, with pronounced subgroup-dependent effects, including alcohol deprivation effects, has clinical validity and may be useful in future investigations of molecular, neuronal, neurochemical, or behavioral adaptations related to excessive alcohol intake as both individuals that escalate their intake over time (HD animals) and low-drinking individuals (LD animals) without escalation are to be distinguished [22].…”

Section: Alcohol Intake Using Three Consecutive Days Of Intermittent mentioning

confidence: 99%

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Subgroup-dependent effects of voluntary alcohol intake on behavioral profiles in outbred Wistar rats

Momeni

Roman

2014

Behavioural Brain Research

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Experimental animal models are critical for understanding the genetic, environmental and neurobiological underpinnings of alcohol use disorders. Limited studies investigate alcohol-induced effects on behavior using free-choice paradigms. The aims of the present experiment were to study voluntary alcohol intake using a modified intermittent access paradigm, investigate the effects of voluntary alcohol intake on behavioral profiles in water- and alcohol-drinking rats, and select extreme low- and high-drinking animals for a more detailed behavioral characterization. Sixty outbred male Wistar rats were randomized into water and alcohol groups. Behavioral profiles in the multivariate concentric square field™ (MCSF) test were assessed prior to and after voluntary alcohol intake. The animals had intermittent access to 20% alcohol and water for three consecutive days per week for seven weeks. The results revealed increased alcohol intake over time. No major alcohol-induced differences on behavior profiles were found when comparing water- and alcohol-drinking animals. The high-drinking animals displayed an alcohol deprivation effect, which was not found in the low-drinking animals. High-drinking rats had lower risk-taking behavior prior to alcohol access and lower anxiety-like behavior after voluntary alcohol intake compared to low-drinking rats. In conclusion, the modified intermittent access paradigm may be useful for pharmacological manipulation of alcohol intake. With regard to behavior, the present findings highlights the importance of studying subgroup-dependent differences and add to the complexity of individual differences in behavioral traits of relevance to the vulnerability for excessive alcohol intake.

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Section: Alcohol Intake Using Three Consecutive Days Of Intermittent mentioning

confidence: 96%

Section: Alcohol Intake Using Three Consecutive Days Of Intermittent mentioning

confidence: 99%

Subgroup-dependent effects of voluntary alcohol intake on behavioral profiles in outbred Wistar rats

Momeni

Roman

2014

Behavioural Brain Research

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“…Various paradigms of alcohol abuse have been proposed representing stages in the development of an AUD including models of voluntary consumption, binge-like ethanol drinking, dependence-like drinking, and relapse-like drinking (Vengeliene et al, 2014, Gilpin et al, 2009, Tabakoff and Hoffman, 2000). Two models that are often used to study pre-dependent ethanol drinking include “drinking in the dark” (DID) and continuous two-bottle choice (C2BC) procedures (Thiele and Navarro, 2014, Ozburn et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Effects of 6 Standard Rodent Diets on Binge-Like and Voluntary Ethanol Consumption in Male C57BL/6J Mice

Marshall

Rinker

et al. 2015

Alcohol Clin Exp Res

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Background In recent years much attention has been given to the lack of reproducibility in biomedical research, particularly in pre-clinical animal studies. This is a problem that also plagues the alcohol research field, particularly in consistent consumption in animal models of alcohol use disorders. One often overlooked factor that could affect reproducibility is the maintenance diet used in pre-clinical studies. Methods Herein, two well-established models of alcohol consumption, the “drinking in the dark” (DID) procedure and the continuous two-bottle choice paradigm (C2BC), were employed to determine the effects of diet on ethanol consumption. Male C57BL/6J were given one of six standard rodent-chow diets obtained from Purina LabDiet®, Inc. [St. Louis, MO; Prolab® RMH 3000] or Harlan Laboratories Inc. [Indianapolis, IN; Teklad Diets T.2916, T.2918, T.2920X, T.7912, or T.8940]. A separate group of animals were used to test dietary effects on ethanol pharmacokinetics and behavioral measures following intraperitoneal (IP) injections of various doses of ethanol. Results Mice eating Harlan diets T.2916 (H2916) and T.2920X (H2920) consumed significantly less ethanol and exhibited lower blood ethanol concentrations (BECs) during DID; however, during C2BC animals maintained on Harlan T.7912 (H7912) consumed more ethanol and had a higher ethanol preference than the other diet groups. Ethanol consumption levels did not stem from changes in alcohol pharmacokinetics, as a separate group of animals administered ethanol IP showed no difference in BECs. However, animals on Harlan diet T.2920X (H2920) were more sensitive to alcohol-induced locomotor activity in an open-field task. No diet dependent differences were seen in alcohol-induced sedation as measured with loss of righting reflex. Conclusions Although these data do not identify a specific mechanism, together they clearly show that the maintenance diet impacts ethanol consumption. It is incumbent upon the research community to consider the importance of describing nutritional information in methods, which may help decrease inter-laboratory reproducibility issues.

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“…In this model, renewed access to alcohol solutions after a period of deprivation for several days leads to a pronounced, although temporary, increase in voluntary alcohol intake in animals (Vengeliene et al, 2014;Eisenhardt et al, 2015). In rats, the ADE was attenuated by repeated subacute treatment with memantine and other NMDAR antagonists (Hölter et al, 2000;Vengeliene et al, 2005Vengeliene et al, , 2008). …”

Section: Introductionmentioning

confidence: 99%

“…However, relapse behavior, as measured by the alcohol deprivation effect (ADE), is attenuated or blocked by several NMDAR and AMPAR antagonists (Hölter et al, 1996;Vengeliene et al, 2005Vengeliene et al, , 2008Sanchis-Segura et al, 2006;Holmes et al, 2013). Thus, there is some indication that glutamate receptors are, at least in part, involved in mediating the addictive properties of alcohol, but the neuroanatomical substrates and the specific contributions of AMPARs and NMDARs are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Glutamate Receptors within the Mesolimbic Dopamine System Mediate Alcohol Relapse Behavior

et al. 2015

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Glutamatergic input within the mesolimbic dopamine (DA) pathway plays a critical role in the development of addictive behavior. Although this is well established for some drugs of abuse, it is not known whether glutamate receptors within the mesolimbic system are involved in mediating the addictive properties of chronic alcohol use. Here we evaluated the contribution of mesolimbic NMDARs and AMPARs in mediating alcohol-seeking responses induced by environmental stimuli and relapse behavior using four inducible mutant mouse lines lacking the glutamate receptor genes Grin1 or Gria1 in either DA transporter (DAT) or D1R-expressing neurons. We first demonstrate the lack of GluN1 or GluA1 in either DAT-or D1R-expressing neurons in our mutant mouse lines by colocalization studies. We then show that GluN1 and GluA1 receptor subunits within these neuronal subpopulations mediate the alcohol deprivation effect, while having no impact on context-plus cue-induced reinstatement of alcohol-seeking behavior. We further validated these results pharmacologically by demonstrating similar reductions in the alcohol deprivation effect after infusion of the NMDAR antagonist memantine into the nucleus accumbens and ventral tegmental area of control mice, and a rescue of the mutant phenotype via pharmacological potentiation of AMPAR activity using aniracetam. In conclusion, dopamine neurons as well as D1R-expressing medium spiny neurons and their glutamatergic inputs via NMDARs and AMPARs act in concert to influence relapse responses. These results provide a neuroanatomical and molecular substrate for relapse behavior and emphasize the importance of glutamatergic drugs in modulating relapse behavior.

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The alcohol deprivation effect model for studying relapse behavior: A comparison between rats and mice

Cited by 162 publications

References 72 publications

Subgroup-dependent effects of voluntary alcohol intake on behavioral profiles in outbred Wistar rats

Subgroup-dependent effects of voluntary alcohol intake on behavioral profiles in outbred Wistar rats

Assessment of the Effects of 6 Standard Rodent Diets on Binge-Like and Voluntary Ethanol Consumption in Male C57BL/6J Mice

Glutamate Receptors within the Mesolimbic Dopamine System Mediate Alcohol Relapse Behavior

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