The Allergens of Cat Epithelia and Cat Serum

Abstract

Cat epithelia extracts were found to contain several electrophoretically separable allergens. Some of these were also detected in cat serum. In addition to serum proteins the epithelia extracts contain allergens with higher electrophoretic mobility than albumin, which was found to be the main allergen of cat serum. The molecular weight of the main cat epithelia allergen was about 32,000. 56 sera from allergic patients were studied by means of the radioallergosorbent test using cat epithelia extracts, a purified epithelia allergen fraction and cat serum as test allergens. About 7% of the patient sera were found to contain IgE specific to cat serum proteins. IgE specific to the epithelia allergen fraction, which was free from serum proteins, was found in 66% of the patient sera, while the rest, about 27%, contained IgE reacting with both kinds of allergens.

Two Newly Identified Cat Allergens: The von Ebner Gland Protein Fel d 7 and the Latherin-Like Protein Fel d 8

“…Since this study did not assume that all cat-allergic subjects would have Fel d 1 as their major allergenic specificity, subjects with the low CAP score of 1 to dander were included so as not to bias the sample to those with a high reactivity to an allergen that predominates in this source. The finding that only 60% of the sera had IgE antibody to Fel d 1 (greater than the mean plus 95% confidence limits of non-cat-allergic titers) is remarkably similar to early studies on epithelial extracts [31,32,33]. Some of the subjects in the current study with binding less than the cutoff did have IgE anti Fel d 1 titers greater than the reaction-negative controls and about 10% of these had reactivity to Fel d 7 or Fel d 8.…”

Comparative Study on Cat Allergens from Fur and Saliva

“…Immunoelectrophoretic and immunodiffusion analysis showed the presence of cat serum albumin. This serum protein could be responsible on the allerginicity of this frac tion and numerous reports indicate that albumin is an important allergen in horse [11] and dog [12] epithelia and to a lesser extent in cat extracts [2,4], The fraction with a mean molecular weight of 40.000 was resolved in two peaks of activity. One of them was associated with the protein band of pi 3.5.…”

“…Using a wide-range pH gradient (3)(4)(5)(6)(7)(8)(9)(10), all the al lergenic components eluted in the pH range 3.9-5 ex cept one with a more acid pi of 3.6. The results are in agreement with those obtained by Varga and Ceska [3] and Stokes and Turner [2] with cat pelt extracts.…”

“…How ever, it seems that the most important allergenic com ponent is 'Cat Allergen One', identified by Ohman et al [1] in cat pelt extract. On the other hand, for some authors [4,5], serum albumin could play a role in the allergenicity of cat pelt. Many immunological studies have been conducted on cat allergenic extracts but limited information is available on the biochemical properties of allergens contained in cat extracts.…”

Häufigkeit Uno Korrelative Beziehung Von Diagnostischen Kriterien Bei Der Tierschuppenallergie

“…4, oben). Besonders zu beriicksichtigen ist hier der unterschiedliche Allergengehalt kommerzieller Tierschuppenextrakte (2,5). Moglicherweise sind hierfiir partielle Antigen-gemeinschaften zwischen den Schuppen verschiedener Tierarten verantwortlich.…”