The alternative splicing of BdTai and its involvement in the development of Bactrocera dorsalis (Hendel)

Liu, Hong; Li, Huimin; Yue, Yong; Song, Zhong-Hao; Wang, Jinjun; Dou, Wei

doi:10.1016/j.jinsphys.2017.07.012

Cited by 11 publications

(12 citation statements)

References 42 publications

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“…It appears that the mRNA levels of LdTai are positively correlated with circulating JH titers in L. decemlineata . Moreover, JH ingestion enhanced the transcription of LdTai in L. decemlineata (Figure 3A), as well as in B. germanica (Lozano et al, 2014) and B. dorsalis (Liu et al, 2017). Upregulation of Tai transcription in response to JH in aforementioned insect species indicates that Tai is the functional receptor of JH.…”

Section: Discussionmentioning

confidence: 96%

“…In holometabolan insects, whether Tai functions as a coactivator of EcR/USP during larval-pupal-adult transition is unknown. In the oriental fruit fly Bactrocera dorsalis , depletion of Tai in larval stage produces no mortality but causes precocious larvae-pupae development (Liu et al, 2017), a typical phenotype in JH deficient insects (Jindra et al, 2013, 2015a). In the red flour beetle Tribolium castaneum (Bitra et al, 2009) and D. melanogaster (Perrimon et al, 1996; Liu et al, 2009; Abdou et al, 2011; König et al, 2011; Jindra et al, 2015b; Wang et al, 2016), RNAi of or mutation in either Met or Tai causes similar larval lethality.…”

Section: Introductionmentioning

confidence: 99%

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Importance of Taiman in Larval-Pupal Transition in Leptinotarsa decemlineata

et al. 2019

Front. Physiol.

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Insect Taiman (Tai) binds to methoprene-tolerant to form a heterodimeric complex, mediating juvenile hormone (JH) signaling to regulate larval development and to prevent premature metamorphosis. Tai also acts as a steroid receptor coactivator of 20-hydroxyecdysone (20E) receptor heterodimer, ecdysone receptor (EcR) and Ultraspiracle (USP), to control the differentiation of early germline cells and the migration of specific follicle cells and border cells in ovaries in several insect species. In holometabolous insects, however, whether Tai functions as the coactivator of EcR/USP to transduce 20E message during larval-pupal transition is unknown. In the present paper, we found that the LdTai mRNA levels were positively correlated with circulating JH and 20E titers in Leptinotarsa decemlineata ; and ingestion of either JH or 20E stimulated the transcription of LdTai . Moreover, RNA interference (RNAi)-aided knockdown of LdTai at the fourth (final) instar stage repressed both JH and 20E signals, inhibited larval growth and shortened larval developing period. The knockdown caused 100% larval lethality due to failure of larval-pupal ecdysis. Under the apolysed larval cuticle, the LdTai RNAi prepupae possessed pupal thorax. In contrast, the process of tracheal ecdysis was uncompleted. Neither JH nor 20E rescued the aforementioned defectives in LdTai RNAi larvae. It appears that Tai mediates both JH and 20E signaling. Our results uncover a link between JH and 20E pathways during metamorphosis in L. decemlineata .

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Importance of Taiman in Larval-Pupal Transition in Leptinotarsa decemlineata

et al. 2019

Front. Physiol.

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“…Tai isoforms, derived from alternative splicing, have been found in other insects. Individual isoforms seem to have distinct contributions to the JH action in metamorphosis and adult reproduction (26)(27)(28). The alternatively included or skipped exons are called insertions and deletions (INDELs) in those studies.…”

Section: Discussionmentioning

confidence: 99%

“…In A. aegypti, Tai is required for the expression of Vg after blood ingestion and is recruited to the Vg promoter via protein interaction with EcR/ USP (25). Isoforms of Tai have been found in the German cockroach Blattella germanica (26), the red flour beetle Tribolium castaneum (26), the migratory locust Locusta migratoria (27), and the oriental fruit fly Bactrocera dorsalis (28). The Tai isoforms in each species differ at the C termini of the proteins, presumably due to alternative splicing.…”

Section: Significancementioning

confidence: 99%

“…The Tai isoforms in each species differ at the C termini of the proteins, presumably due to alternative splicing. Depletion of individual Tai isoforms has indicated that each isoform has a distinct contribution to the antimetamorphic action of JH in B. germanica and B. dorsalis (26,28). In L. migratoria, the JH-regulated ovarian development and oocyte maturation rely heavily on a single Tai isoform containing an insertion of a histidine-and proline-rich motif (27).…”

Section: Significancementioning

confidence: 99%

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Juvenile hormone-regulated alternative splicing of thetaimangene primes the ecdysteroid response in adult mosquitoes

Liu

Zhu

2018

Proc. Natl. Acad. Sci. U.S.A.

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Juvenile hormone (JH) regulates many aspects of insect development and reproduction. In some processes, JH plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20E). In mosquitoes, JH prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20E after blood ingestion. The molecular basis of this competence is still not well understood. Here, we report that JH regulates pre-mRNA splicing of the gene, which encodes a key transcriptional regulator required for both JH- and 20E-controlled gene expression. JH stimulated the production of the Taiman isoforms A/B, while reducing the levels of the isoforms C/D, in the fat body after adult eclosion. The appearance of the A/B isoforms in maturing mosquitoes was accompanied by acquisition of the competence to respond to 20E. Depletion of the A/B isoforms, by inhibiting the alternative splicing or by isoform-specific RNA interference, considerably diminished the 20E-induced gene expression after a blood meal and substantially impaired oocyte development. In accordance with this observation, further studies indicated that in the presence of 20E, the Taiman A/B isoforms showed much stronger interactions with the 20E receptor complex than the Taiman C/D isoforms. In contrast, all four isoforms displayed similar capabilities of forming active JH receptor complexes with the methoprene-tolerant protein (Met). This study suggested that JH confers the competence to newly emerged female mosquitoes by regulating mRNA splicing to generate the Taiman isoforms that are essential for the vitellogenic 20E response.

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RNA interference of Argonaute‐1 delays ovarian development in the oriental fruit fly, Bactrocera dorsalis (Hendel)

Yang

Zhang

Fan

et al. 2021

Pest Management Science

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BACKGROUND With the development of rapid resistance, new modes of action for pesticides are needed for insect control, such as RNAi‐based biopesticides targeting essential genes. To explore the function of Argonaute‐1 (Ago‐1) and potential miRNAs in ovarian development of Bactrocera dorsalis, an important agricultural pest, and to develop a novel control strategy for the pest, BdAgo‐1 was first identified in B. dorsalis. RESULTS Spatiotemporal expression analysis indicated that BdAgo‐1 had a relatively high transcriptional level in the ovarian tissues of adult female B. dorsalis during the sexual maturation period. RNA interference (RNAi) experiment showed that BdAgo‐1 knockdown significantly decreased the expression levels of ovarian development‐related genes and delayed ovarian development. Although RNAi‐mediated silencing of Ago‐1 led to a reduced ovary surface area, a subsequent oviposition assay revealed that the influence was minimal over a longer time period. Small RNA libraries were constructed and sequenced from different ovarian developmental stages of B. dorsalis adults. Among 161 identified miRNAs, 84 miRNAs were differentially expressed during the three developmental stages of the B. dorsalis ovary. BdAgo‐1 silencing caused significant down‐regulation of seven differentially expressed miRNAs (DEMs) showing relatively high expression levels (>1000 TPM (Transcripts per kilobase of exon model per million mapped reads)). The expression patterns of these seven core DEMs and their putative target genes were analyzed in the ovaries of B. dorsalis. CONCLUSION The results indicate that Ago‐1 and Ago‐1‐dependent miRNAs are indispensable for normal ovarian development in B. dorsalis and help identify miRNA targets useful for control of this pest.

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The alternative splicing of BdTai and its involvement in the development of Bactrocera dorsalis (Hendel)

Cited by 11 publications

References 42 publications

Importance of Taiman in Larval-Pupal Transition in Leptinotarsa decemlineata

Importance of Taiman in Larval-Pupal Transition in Leptinotarsa decemlineata

Juvenile hormone-regulated alternative splicing of thetaimangene primes the ecdysteroid response in adult mosquitoes

RNA interference of Argonaute‐1 delays ovarian development in the oriental fruit fly, Bactrocera dorsalis (Hendel)

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