The Alternatively Spliced Exon of the Platelet-Derived Growth Factor A Chain Encodes a Nuclear Targeting Signal

Maher, D. W.; Lee, B A; Donoghue, Daniel J.

doi:10.1128/mcb.9.5.2251-2253.1989

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…After the first description of internalization of [ 3 H] labelled TRH to the nucleus (Gourdji et al, 1973) many others peptides have been detected in the nucleus of target cells (Rakowicz-Szulczynska et al, 1986;Smith and Jarett, 1987;Smith et al, 1988;Maher et al, 1989;Soler et al, 1989;Baldin et al, 1990;Morel, 1994;Wiedlocha et al, 1994Wiedlocha et al, , 1996Maher, 1996;Jans et al, 1997) including Growth hormone (GH) and its receptor (GHR). After its binding to the plasma membrane receptor, GH is internalized in different cellular compartments (Roupas and Herington, 1989;Ilondo et al, 1992;.…”

Section: Introductionmentioning

confidence: 99%

Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

Morel¹

2023

Preprint

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Growth hormone (GH) and many other hormones or growth factors have been shown to be rapidly internalized and translocated into the nucleus. The first event of peptide action is binding to its receptor which initiates both signal transduction pathways and internalization. The latter process involves the nucleus and probably gene transcription. In order to analyze the consequences of internalization of GH on gene expression, we used different populations of CHO cells, transfected with either full length GH receptor, or with defective receptor unable to trigger either signal transduction (deletion of box1) or the internalization of GH (Phe346mutation in Ala). In addition, functional isolated nuclei were incubated 10 and 30 min with 50 nM GH in order to analyze the direct effect of GH on gene expression without surrounding cytoplasmic structures. The genes involved in signal transduction pathways were not revealed if GH internalization is the only functional activity in the whole cell as well as with isolated nuclei. In intact cell, internalization increased expression of 297 genes and decreased fewer than 10% of those known to be influenced by GH. Variations of expression in purified nuclei showed large variations with time. If cell signaling was not modified, cellular growth and proliferation, nucleic acid metabolism, cellular development, cell cycle and gene expression showed many variations with time. GH internalization shows direct effects on gene expression, different from those stimulated by signal transduction.

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Section: Introductionmentioning

confidence: 99%

Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

Morel¹

2023

Preprint

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“…Maher et al (11) recently have shown that the nuclear targeting signal of the A chain of platelet-derived growth factor is dependent on alternative splicing. Thus, bFGF represents another example of an endogenous growth factor with two possible subcellular locations regulated at the posttranscriptional level.…”

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confidence: 99%

Alternative Initiation of Translation Determines Cytoplasmic or Nuclear Localization of Basic Fibroblast Growth Factor

Bugler

Amalríc

Piégay

1991

Molecular and Cellular Biology

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Three forms of basic fibroblast growth factor (bFGF), initiated at an AUG (18 kDa) and two CUG (21 and 22.5 kDa) start codons, were produced following transfection of COS cells with human hepatoma bFGF cDNA. The subcellular localization of the different forms was investigated directly or by using chimeric genes constructed by fusion of the bFGF and chloramphenicol acetyltransferase open reading frames. The AUG-initiated proteins were cytoplasmic, while the CUG-initiated forms were nuclear. The signal sequence responsible for the nuclear localization of bFGF is contained within 37 amino acid residues between the second CUG and the AUG start codons. Alternative initiation of translation regulates the subcellular localization of bFGF and thus could modulate its role in cell growth and differentiation control.

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Cloning and molecular characterization of a novel gene strongly induced by the adenovirus E1A gene in rat thyroid cells

et al. 2003

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The Alternatively Spliced Exon of the Platelet-Derived Growth Factor A Chain Encodes a Nuclear Targeting Signal

Cited by 3 publications

References 22 publications

Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

Alternative Initiation of Translation Determines Cytoplasmic or Nuclear Localization of Basic Fibroblast Growth Factor

Cloning and molecular characterization of a novel gene strongly induced by the adenovirus E1A gene in rat thyroid cells

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