The ameliorative impacts of curcumin on copper oxychloride-induced hepatotoxicity in rats

Abstract

Background: Copper oxychloride (COC) (50% of its component, copper) is copper-based fungicides. The present study aimed to investigate the possible protective effect of 80 mg/kg curcumin against the toxicity of 500, 1000, or 2000 mg COC per kilogram body weight for 90 days on the liver of a rat. Serum glutamic-pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SGOT), hepatic glutathione reduced content (GSH), and malondialdehyde (MDA) levels were detected. The histological and ultrastructure changes of the liver tissues as well as the hepatic content of copper, iron, manganese, and zinc were also reported. Results: COC-treated rats showed an increase of SGPT and SGOT, with the elevation of copper and zinc content and MDA levels with no change in GSH level. The liver showed a significant increase in the copper and iron contents. The liver of COC-treated rats showed histological and ultrastructural damage that increased with increasing the COC dose. Conversely, curcumin supplementation potentially recovered liver function enzymes in only low doses of COC, reduced MDA level, increased GSH content, and improved the hepatic lesions. These findings revealed that subchronic exposure to even low levels of COC may have potential hazards and harmful effects on the liver, and the curcumin markedly attenuated the COC biochemical, histological, and cellular alterations in liver tissues, best with the low dose of COC. Conclusions: It is concluded that curcumin has a limited protective role against COC liver toxicity.

Study of the toxic effect and safety of vitamin E supplement in male albino rats after 30 days of repeated treatment

“…Afterwards, it was rinsed with distilled water, dehydrated in graded alcohol, cleared in xylene, and embedded in paraffin. Finally, they were cut into 5 μm section with a rotary microtome and stained with haematoxylin and eosin and examined under a microscope (Fischer et al., 2008; El-Hak and Mobarak, 2018; El-Hak et al., 2018). A minimum of three fields for each slide were examined and scored semiquantitatively for severity of changes unaware of the type of treatment.…”

“…The serum separated was used for various parameters such as creatinine, urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxidation (Malondialdehyde) and reduced GSH by using commercial kits (El-Hak and Mobarak, 2018), Serum testosterone which was quantitatively measured by adopting enzyme-linked immunosorbent assay (ELISA) technique using kits purchased from Rapid Labs Ltd., (Essex, U.K). Malondialdehyde formed from the breakdown of polyunsaturated fatty acids that served as a convenient index for determining lipid peroxidation that reacts with thiobarbituric acid to give a red color absorbing at 535 nm (Draper and Hadley, 1990).…”

Pterocarpus mildbraedii(Harms) extract resolves propanil‐induced hepatic injury via repression of inflammatory stress responses in Wistar rats

“…The role of pesticides in the etiology of liver ailments has been well‐documented (Karami‐Mohajeri et al., 2017; Manfo et al., 2020). For example, in animal studies, rats exposed to the copper‐based fungicides, fipronil, and paraquat dichloride presented with oxidative damage and liver injury (Gad El‐Hak & Mobarak, 2018; Kartheek & David, 2018; Ujowundu et al., 2018). Other pesticides, such as imidacloprid (Arfat et al., 2014) and Fenitrothion (Ehsan et al., 2014), caused a hepatic injury in mice and fish models of animal experimentation, respectively.…”

Histological study on the acute injection of aluminum sulfate on different organs of rats

“…Then, Rats were sacrificed by decapitation. Part of the forebrains, heart, lung, liver, kidney, spleen, stomach, small intestine epidydimis, testes, uterus and ovary were isolated and fixed in 10 % of phosphate buffered formalin for 2 days for histological preparation for light microscopic examination [EL-Hak, Moustafa [15,16,17]. 0.5 g of each rat tissue was used for the assessment of aluminum concentrations [18].…”