The aminergic control of cockroach salivary glands

Baumann, Otto; Krach, Christian; Baumann, Arnd; Blenau, Wolfgang

doi:10.1002/arch.20128

Cited by 57 publications

(54 citation statements)

References 69 publications

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“…concentration and possibly also in the cAMP concentration in salivary duct cells (Hille and Walz et al, 2006). Because an increase in cAMP concentration activates VATPase in Calliphora salivary glands (Dames et al, 2006), we have therefore asked whether a drop in pH i is sufficient to stimulate V-ATPase-mediated outward H + pumping in Periplaneta salivary duct epithelial cells.…”

Section: +mentioning

confidence: 99%

A vacuolar-type H+-ATPase and a Na+/H+exchanger contribute to intracellular pH regulation in cockroach salivary ducts

Hille

2007

Journal of Experimental Biology

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SUMMARY Cells of the dopaminergically innervated salivary ducts in the cockroach Periplaneta americana have a vacuolar-type H+-ATPase(V-ATPase) of unknown function in their apical membrane. We have studied whether dopamine affects intracellular pH (pHi) in duct cells and whether and to what extent the apical V-ATPase contributes to pHiregulation. pHi measurements with double-barrelled pH-sensitive microelectrodes and the fluorescent dye BCECF have revealed: (1) the steady-state pHi is 7.3±0.1; (2) dopamine induces a dose-dependent acidification up to pH 6.9±0.1 at 1 μmol l–1 dopamine, EC50 at 30 nmol l–1dopamine; (3) V-ATPase inhibition with concanamycin A or Na+-free physiological saline (PS) does not affect the steady-state pHi; (4)concanamycin A, Na+ -free PS and Na+/H+exchange inhibition with 5-(N-ethyl-N-isopropyl)-amiloride(EIPA) each reduce the rate of pHi recovery from a dopamine-induced acidification or an acidification induced by an NH4Cl pulse; (5)pHi recovery after NH4Cl-induced acidification is almost completely blocked by concanamycin A in Na+-free PS or by concanamycin A applied together with EIPA; (6) pHi recovery after dopamine-induced acidification is also completely blocked by concanamycin A in Na+-free PS but only partially blocked by concanamycin A applied together with EIPA. We therefore conclude that the apical V-ATPase and a basolateral Na+/H+ exchange play a minor role in steady-state pHi regulation but contribute both to H+extrusion after an acute dopamine- or NH4Cl-induced acid load.

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Section: +mentioning

confidence: 99%

A vacuolar-type H+-ATPase and a Na+/H+exchanger contribute to intracellular pH regulation in cockroach salivary ducts

Hille

2007

Journal of Experimental Biology

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“…These biogenic amines were believed is vital in physiological events of salivary glands [5]. In cockroach, salivation is regulated by the salivary duct nerve that consists of two neurons: dopaminergic and serotonergenic [6][7][8]. Both of these compounds were found to stimulate the salivation in isolated SG [9][10].…”

Section: Introductionmentioning

confidence: 99%

Morphology of salivary gland and distribution of dopamine and serotonin on red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae)

Hidayah

Wahida²,

Shafinaz³

et al. 2013

AIP Conference Proceedings

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Abstract. The Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier, 1790) is insect pest to plants of the family Palmaceae. No study has been reported on the digestive mechanism of Red Palm Weevil (RPW). Salivary glands are responsible in the feeding regulation of insect while serotonin and dopamine play a significant role in the regulation of this gland. It is great to see the morphology of the salivary gland and how dopamine and serotonin possibly play their role in this gland. Two variation of RPW, striped and spotted RPW were chosen. The morphology of the gland of both RPW variants examined by using light microscopy was found to be a tubular type. Immunohistochemical analysis conducted showed that serotonin and dopamine in both variations did not innervate the glands suggesting they are not act as neurotransmitter. However, it can be detected on few areas within the glands. This suggests that serotonin and dopamine may act as a hormone because there is no evidence on the nerve fibers. The role of these biogenic amines in the salivary gland of RPW needs further investigation. Hopefully the data would help in understanding the mechanism of salivary glands control by biogenic amines in RPW specifically and insects with sucking mouthpart generally.

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“…Biogenic amines [octopamine (OA), serotonin (5-HT) and dopamine (DA)] regulate various functions in the CNS of invertebrates, serving as neurotransmitters and neuromodulators (Walz et al, 2006). In the cockroach R. maderae, injections of OA or its agonist synephrine caused a significant increase in both protein and carbohydrate feeding (Cohen et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Dopamine as an anorectic neuromodulator in the cockroachRhyparobia maderae

Allen

Kummer

Cohen

2011

Journal of Experimental Biology

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SUMMARYInsects, including cockroaches, self-select a balanced diet when faced with different nutrient choices. For self-selection to be carried out effectively, insects possess neuroregulatory systems to control their food intake. In the present study, we examined the role of the neurotransmitter dopamine (DA) in the feeding regulation of the Madeira cockroach (Rhyparobia maderae). When R. maderae nymphs were injected with 20l of 100mmoll -1 DA, they showed an 83.3% reduction in sucrose intake and a 78.9% reduction in total intake compared with saline-injected controls. The DA agonist, 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (6,7-ADTN) (100mmoll -1 in 1l), caused a significant reduction in sucrose feeding, reducing feeding by 47.3% compared with saline-injected controls. Protein feeding was also significantly reduced by 6,7-ADTN to 62%. Rhyparobia maderae nymphs injected with the DA antagonist chlorpromazine (100mmoll -1 in 1l) did not differ significantly from control nymphs in their feeding behavior. Interestingly, R. maderae nymphs injected with 2l or 5l chlorpromazine (100mmoll -1 ) showed significantly increased mortality rates of 47.5% or 66.7%, respectively. The DA antagonist, spiperone (100mmoll -1 in 1l), caused a significant feeding response, showing an increase in feeding in both sucrose (310.6%) and total intake (236.3%). Casein feeding in R. maderae nymphs was also elevated (70.8%) but this was not statistically significant. The experiments with DA, the DA agonist 6,7-ADTN and the DA antagonist spiperone strongly suggest that the neurotransmitter DA is involved in regulating feeding in the cockroach R. maderae.

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The aminergic control of cockroach salivary glands

Cited by 57 publications

References 69 publications

A vacuolar-type H+-ATPase and a Na+/H+exchanger contribute to intracellular pH regulation in cockroach salivary ducts

A vacuolar-type H+-ATPase and a Na+/H+exchanger contribute to intracellular pH regulation in cockroach salivary ducts

Morphology of salivary gland and distribution of dopamine and serotonin on red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae)

Dopamine as an anorectic neuromodulator in the cockroachRhyparobia maderae

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