The Amino- and Carboxyl-terminal Residues and the Self-digestion of Bovine Pancreatic Carboxypeptidase A

Amino acid sequence of thermolysin. Isolation and characterization of the fragments obtained by cleavage with cyanogen bromide

“…Cyanogen Bromide Cleavage. Cleavage with cyanogen bromide was carried out by the method of Gross and Witkop (1962) as modified by Bargetzi et al (1964) and Sampath . Approximately 500 mg of purified and denatured thermolysin were dissolved in a mixture of 30 ml of purified trifluoroacetic acid and 10 ml of water.…”

Phospholipase A2 and its zymogen from porcine pancreas. VI. Histidine at the active site of phospholipase A2

“…Cyanogen Bromide Fragmentation. Cyanogen bromide cleavage of [14C]-p-bromophenacyl bromide treated S-sulfophospholipase A2 was performed in 70% trifluoroacetic acid as described by Bargetzi et al (1964).…”

Characterization of Bovine Carboxypeptidase A (Allan)

“…The predominant component, ß, has the amino-terminal sequence Ser-Thr-Asn-Thr-Phe-Asn-Tyr-Ala-. Minor components are carboxypeptidase Aa T JL hree methods of preparation of bovine carboxypeptidase A have been described in the literature and the resulting products denoted according to originators are CPA (Anson), CPA (Cox), and CPA(Allan) (Bargetzi et al, 1964). Each of these preparations contains the a, ß, and y forms of the enzyme in varying proportions.…”

“…The composition of CPA (Allan) seems less certain. The amino-terminal residue was reported to be the same as that of CPA(Anson), /.<?., asparagine (Coombs et al, 1964;Bargetzi et al, 1964). The Allan enzyme, however, had a higher solubility and was more completely reactivated on addition of zinc to the apoenzyme than the Anson preparation (Vallee et al, 1960).…”