The aminopeptidase from hog intestinal brush border

Maroux, S.; Louvard, Daniel; Baratti, J.

doi:10.1016/0005-2744(73)90083-1

Cited by 502 publications

(301 citation statements)

References 25 publications

Supporting

Mentioning

293

Contrasting

Unclassified

Order By: Relevance

“…Maltase and sucrase activities were measured according to Dahlqvist (1964), using maltose and sucrose as substrates, respectively. The activity of aminopeptidase N (APN) was determined according to Maroux et al (1973). The method described by Pierce and Suelter (1977) was used to estimate the protein concentration of mucosa samples.…”

Section: Small Intestinal Enzyme Activities Determinationmentioning

confidence: 99%

Adaptability of the digestive function according to age at weaning in the rabbit: I. Effect on feed intake and digestive functionality

Gallois

Huërou‐Luron

Fortun-Lamothe

et al. 2008

Animal

View full text Add to dashboard Cite

The functional adaptability of the digestive system to the level of feed intake was investigated in the young rabbits by comparing two groups of 12 litters each, weaned at 21 (W21) or 35 (W35) days of age. From 14 days onwards, rabbits were fed a pelleted feed (NDF: 332 g/kg, CP: 177 g/kg, starch: 98 g/kg, as-fed basis). Until 49 days of age, the profile of digestive enzymes was weekly determined in the small intestinal content and mucosa, as well as caecal fermentation traits and fibrolytic activities. In the W21 group, the solid feed intake was increased by 57% between 21 and 35 days (P , 0.01), while the daily body growth was lower from 21 till 42 days (217%, P , 0.05) when compared with the W35 group. Activities of enzymes of pancreatic origin were only scarcely influenced by the weaning age. In the W21 group, amylase activity tended to be lower at 28 days of age (236%, P 5 0.064), and trypsin activity was decreased by 31% at 49 days of age (P , 0.01). Lipase activity was similar in both weaning groups. Duodenal and jejunal activities of maltase and aminopeptidase N (APN) were higher on day 28 in the W21 group as compared with the W35 group (31.4 to 32.4, respectively, P , 0.05). On day 35, duodenal APN activity was twice as higher in the W21 group than in the W35 group (P , 0.01). In caecum, major differences between both weaning groups were observed at 28 days of age with a decrease in ammonia concentration (243%, P , 0.01) in W21 compared with W35 rabbits. Conversely, the acetate proportion was 5% higher in the W21 group (P , 0.01) on day 28. In conclusion, the digestive tract of early-weaned rabbits showed some adaptative properties in response to nutritional environment changes, but they were insufficient to maintain their growth rate.

show abstract

Section: Small Intestinal Enzyme Activities Determinationmentioning

confidence: 99%

Adaptability of the digestive function according to age at weaning in the rabbit: I. Effect on feed intake and digestive functionality

Gallois

Huërou‐Luron

Fortun-Lamothe

et al. 2008

Animal

View full text Add to dashboard Cite

show abstract

“…This technique has been also tested with the anti-aminopeptidase antibody, from intestinal brush border membrane, purified as described previously [7] on immunoadsorbent column of aminopeptidase coupled to indubiose. Identical results were obtained although the number of antigenic determinants is twice that of the alkaline phosphatase ofE.…”

Section: Discussionmentioning

confidence: 99%

“…Alkaline phosphatase was prepared from E. coli C 90 [6] and aminopeptidase was purified from pig intestinal mucosa as previously described [7].…”

Section: Protein Antigensmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of active iodinated specific antibodies

Pagès¹,

Louvard²,

Lazdunski³

1975

FEBS Letters

View full text Add to dashboard Cite

“…Sucrase and maltase were assayed according to Dahlqvist (1968), leucine aminopeptidase according to Maroux et al (1973) (Vasseur et al, 1979 Sladen and Harries, 1972 ;Teem and Phillips, 1972). These in vivo functional effects on the digestive and absorptive processes were probably not due to gross morphological damage.…”

mentioning

confidence: 99%