The relationship between surface tension and release of rat jejunal brush border membrane hydrolases induced by sodium deoxycholate

Vasseur, Monique; Pousse, A.; Férard, G

doi:10.1051/rnd:19800805

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…In this way the light microscopic detection of the reaction product might be critically affected. Thus, redistribution possibly accounts for the decreased reactions of surface enzymes (ALP and MgATPase) in the present experiments, particularly since their concentration may already have been critically reduced as a result of the solubilizing and extracting effect of DOC (2,3,5,6,36).…”

Section: Discussionmentioning

confidence: 89%

Enzyme changes in remodelling epithelial cells: A histochemical study of the rat jejunum in vivo during and following exposure to deoxycholic acid

Matovelo¹,

Landsverk²,

Sund³

1993

APMIS

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Loops of rat jejunum were exposed in vivo to different concentrations of deoxycholic acid (DOC; 0, 2.5, 5, 10 and 20 mM). Following a 30 min exposure period, DOC was washed out of the loops and the intestines were allowed to recover for 15 or 150 min. Frozen tissue for enzyme histochemistry was collected during exposure and following the recovery periods. As shown previously, exposure to DOC caused a dose‐dependent loss of epithelial cells at the villous tips and denudation of the lamina propria. Flattened epithelial cells bordering the denuded areas were, however, responsible for a rapid restoration of epithelial continuity, which was completed within 15 min. In the present study, these flattened cells showed normal reactivity for non‐specific esterase and succinate dehydrogenase. In contrast, following a prolonged recovery period (150 min), a subpopulation of enterocytes at the villous tips that otherwise appeared normal showed decreased reactivity for brush border enzymes and non‐specific esterase, and a positive reaction for mucin. A shutdown in the synthesis of cytoplasmic enzymes and redistribution of cell surface enzymes could be responsible for these late occuring enzyme changes, that were consistently observed after 150 min of recovery from DOC at 20 mM. Alternatively, retention of goblet cells and/or a modification in enzyme synthesis may explain the presence of mucin that was demonstrated in the epithelial cells which had low enzyme reactivity.

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Section: Discussionmentioning

confidence: 89%

Enzyme changes in remodelling epithelial cells: A histochemical study of the rat jejunum in vivo during and following exposure to deoxycholic acid

Matovelo¹,

Landsverk²,

Sund³

1993

APMIS

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“…The inhibition of glu cose may be also due to an interaction of the bile salt with the carrier in the brush border membrane. In effect, a 1-mmol/1 deoxycho late perfusion produced significant release of some brush border hydrolases (25). Finally, the location of deoxycholate inhibition of glucose transfer requires further clarifica tion.…”

Section: Glucose Absorptionmentioning

confidence: 99%

Effect of Sodium Deoxycholate on Intestinal Glucose Absorption and (Na+-K+)-ATPase in the Rat

Férard¹,

Galluser²,

Sall³

et al. 1980

Enzyme

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Action Mechanisms of Secretagogue Drugs

Nell¹,

Rummel²

1984

Pharmacology of Intestinal Permeation II

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The relationship between surface tension and release of rat jejunal brush border membrane hydrolases induced by sodium deoxycholate

Abstract: Summary. Rat 4. The maximum releasing effect also coincided with the critical micellar concentration of deoxycholate (1.5-2.5 mM).Introduction.

Cited by 3 publications

References 7 publications

Enzyme changes in remodelling epithelial cells: A histochemical study of the rat jejunum in vivo during and following exposure to deoxycholic acid

Enzyme changes in remodelling epithelial cells: A histochemical study of the rat jejunum in vivo during and following exposure to deoxycholic acid

Effect of Sodium Deoxycholate on Intestinal Glucose Absorption and (Na+-K+)-ATPase in the Rat

Action Mechanisms of Secretagogue Drugs

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