The Amphoteric Behavior of Bovine Plasma Albumin. Evidence for Masked Carboxylate Groups in the Native Protein<sup>*</sup>

Vijai, Kamala Kant; Foster, Joseph F.

doi:10.1021/bi00856a027

Cited by 71 publications

(44 citation statements)

References 20 publications

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“…It has recently been established (Vijai & Foster, 1967) (Dubuisson 1939). Figure 1 B-D shows the time course of change in ionic concentration in 2 times tonicity.…”

Section: Resultsmentioning

confidence: 92%

The effect of hypo‐ and hypertonic solutions on volume and ion distribution of smooth muscle of guinea‐pig taenia coli

Brading¹,

Setekleiv²

1968

The Journal of Physiology

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SUMMARY1. The intra-and extracellular spaces and ionic content of the taenia coli of the guinea-pig have been measured in a series of bathing solutions in which the tonicity varied from 0.5 to 3 times the tonicity of the normal Krebs solution.2. Equilibrium of the tissue in the experimental solution is reached within about 30 min.3. The absolute values of the parameters measured have been shown to depend on the blotting technique used. These differences were eliminated by expressing the results as a percentage of the values found in the normal Krebs solution.4. In hypertonic solutions the cell behaves as a perfect osmometer, the cell volume changing in proportion to the tonicity of the bathing medium. Only a small amount of cations is lost from the cells. A considerable amount of chloride is lost, making the postulation of its replacement by some other anions necessary in order to maintain electroneutrality of the intracellular solution.5. In hypotonic solutions the cells do not behave as predicted for a perfect osmometer. In 0 5 hypotonic solution an actual decrease in cell volume was observed associated with an increase of the extracellular space probably due to penetration of [14C]sorbitol into the cell. The intracellular ionic concentration was decreased. These findings suggest damage of the cell membrane.6. The observed hyperpolarization of the membrane in hypertonic solution can be explained by the increased intracellular potassium concentration.

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“…It has recently been established (Vijai & Foster, 1967) (Dubuisson 1939). Figure 1 B-D shows the time course of change in ionic concentration in 2 times tonicity.…”

Section: Resultsmentioning

confidence: 92%

The effect of hypo‐ and hypertonic solutions on volume and ion distribution of smooth muscle of guinea‐pig taenia coli

Brading¹,

Setekleiv²

1968

The Journal of Physiology

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“…A partial opening of compact domain regions open symbols show the amount of HSA and heparin deter-was suggested by the increased accessibility of some nonpomined on dry polyethylene samples soaked with PBS for lar amino acid residues to solvent (26) and by the increased various time intervals.) The crosslinked samples that were titratability of about 40 carboxyl groups (27). soaked for 12 h in PBS were dried, soaked again in PBS Heparin (28) is a mucopolysaccharide composed of refor 2 h, and dried.…”

Section: Discussionmentioning

confidence: 99%

Multiple Alternating Molecular Layers of Albumin and Heparin on Solid Surfaces

Brynda

Houska

1996

Journal of Colloid and Interface Science

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“…1). From the number of amino acid residues determined in the present study, 585, and a total number of carboxyl groups of 100 (24,25) Apo' of 585 = 0.015 g/ml.…”

Section: Density Gradient Experimentsmentioning

confidence: 73%

“…From the determined Oo'S it is possible to es-timate the extend of hydration or binding of anions and cations to the proteins. In order to get a better understanding of the contribution from the individual ionizable groups to hydration and ion binding, a series of experiments was initiated in which the buoyant titration curves for synthetic polypeptides (1,21) and proteins (13,20,25) were determined. The present article is a contribution to these studies.…”

Section: Introductionmentioning

confidence: 99%

Buoyant titration of bovine mercaptalbumin chemically modified at the carboxyl groups

Svendsen

1976

Carlsberg Res. Commun.

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Bovine mercaptalbumin has been modified at the carboxyl groups by means of I-ethyl-3 (3-dimethylisopropyl) carbodiimide and glycine amide. A stable derivative was obtained after modification of 54 groups. The modified protein differed only slightly from the unmodified one with respect to the optical rotatory dispersion parameters ao, bo and ~. This preparation was studied in the analytical ultracentrifuge in a CsCI gradient in the pH-interval from 2 to 1 I. The buoyant densities were determined and compared with those of the unmodified protein in the same pH-interval. The contribution from the carboxyl groups to the buoyant density is discussed and compared with results obtained with other proteins and synthetic polypeptides.

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The Amphoteric Behavior of Bovine Plasma Albumin. Evidence for Masked Carboxylate Groups in the Native Protein^*

Cited by 71 publications

References 20 publications

The effect of hypo‐ and hypertonic solutions on volume and ion distribution of smooth muscle of guinea‐pig taenia coli

The effect of hypo‐ and hypertonic solutions on volume and ion distribution of smooth muscle of guinea‐pig taenia coli

Multiple Alternating Molecular Layers of Albumin and Heparin on Solid Surfaces

Buoyant titration of bovine mercaptalbumin chemically modified at the carboxyl groups

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The Amphoteric Behavior of Bovine Plasma Albumin. Evidence for Masked Carboxylate Groups in the Native Protein*

Cited by 71 publications

References 20 publications

The effect of hypo‐ and hypertonic solutions on volume and ion distribution of smooth muscle of guinea‐pig taenia coli

The effect of hypo‐ and hypertonic solutions on volume and ion distribution of smooth muscle of guinea‐pig taenia coli

Multiple Alternating Molecular Layers of Albumin and Heparin on Solid Surfaces

Buoyant titration of bovine mercaptalbumin chemically modified at the carboxyl groups

Contact Info

Product

Resources

About

The Amphoteric Behavior of Bovine Plasma Albumin. Evidence for Masked Carboxylate Groups in the Native Protein^*