The Anaerobic Monotrichous Butyric Acid-Producing Curved Rod-Shaped Bacteria of the Rumen

Bryant, M. P.; Small, Nola

doi:10.1128/jb.72.1.16-21.1956

Cited by 225 publications

(151 citation statements)

References 8 publications

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“…The bacterium B. fibrisolvens strain D1 has been the subject of numerous studies because it is the type strain of an important species in the rumen bacterial population (Gill and King 1958;Diez-Gonzalez et al 1999). Our findings are in accordance with those of Bryant and Small (1956) showing that the main product of fermentation of glucose by strain D1 is butyric acid. The lower content of this acid obtained in our studies can be the result of differences in culture as well as in assay methods.…”

Section: Discussionsupporting

confidence: 90%

“…The ability of B. fibrisolvens to ferment inulin was first reported by Bryant and Small (1956) and then by Brown and Moore (1960) and Margherita et al (1964). This suggests that bacteria synthesize at least an enzyme(s) capable of digesting inulin-type fructans.…”

Section: Introductionmentioning

confidence: 95%

“…The species Butyrivibrio fibrisolvens was described by Bryant and Small (1956) as a butyrate-producing curved rod-shaped rumen bacteria able to digest and then to ferment the majority of polysaccharides occurring in ruminant diets, i.e., dextrin, starch, xylan and in minor amounts, cellulose. Since then, amylolytic, xylanolytic and even pectinolytic properties of strains belonging to this species have been documented in numerous studies (Wojciechowicz et al 1982;Stewart et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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The fructanolytic abilities of the rumen bacteriumButyrivibrio fibrisolvensstrain 3071

et al. 2015

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Aims To determine if Butyrivibrio fibrisolvens strain 3071 is able to use fructose polymers for growth and to identify the enzymes involved in their digestion. Methods and Results Strain 3071 utilized 97, 89, 85 and 60% of sucrose, timothy grass fructan, inulin oligosaccharides and inulin, respectively, in the growth medium. A cell extract from timothy grass fructan‐grown bacteria was used for identification of fructanolytic enzymes by anion exchange chromatography, gel filtration, zymography and thin‐layer chromatography. The bacterium synthesizes a specific endolevanase and a nonspecific β‐fructofuranosidase. Both enzymes occurred in two forms differing in molecular weight. The β‐fructofuranosidase was not able to digest long‐chain inulin or timothy grass fructan, but degraded inulin oligosaccharides and sucrose. Addition of 1,4‐dithioerythritol to an enzyme solution did not affect the activity of endolevanase(s), but increased the ability of β‐fructofuranosidase to digest sucrose. The digestion of timothy grass fructan by endolevanase(s) was described by Michaelis–Menten kinetics in which Km = 2·82 g l−1 and Vmax = 4·01 μmoles reducing sugar equivalents × mg−1 × min−1. Conclusion Strain 3071 synthesizes enzymes enabling it to use grass fructans for growth. Significance and Impact of the Study Butyrivibrio fibrisolvens strain 3071 can be considered a member of the rumen fructanolytic guild.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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The fructanolytic abilities of the rumen bacteriumButyrivibrio fibrisolvensstrain 3071

et al. 2015

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“…Although many ruminal bacteria ferment pentoses, there has been relatively little study devoted to pentose sugar utilization. Butyrivibrio fibrisolvens is a predominant ruminal organism that has also been isolated from fecal material of non-ruminants and from anaerobic digesters [1,2]. All characterized strains are xylanolytic, and hemicellulose degradation may be an important niche for this bac-D-isomer of xylose and L-isomer of arabinose were used in all experiments.…”

Section: Introductionmentioning

confidence: 99%

Pentose transport by the ruminal bacterium Butyrivibrio fibrisolvens

Strobel

1994

FEMS Microbiology Letters

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Butyrivibrio fibrisolvens is a fibrolytic ruminal bacterium that degrades hemicellulose and ferments the resulting pentose sugars. Washed cells of strain D1 accumulated radiolabelled xylose (Km = 1.5 microM) and arabinose (Km = 0.2 microM) when the organism was grown on xylose, arabinose, or glucose, but cultures grown on sucrose or cellobiose had little capacity to transport pentose. Glucose and xylose inhibited transport of each other non-competitively. Both sugars were utilized preferentially over arabinose, but since they did not inhibit transport of arabinose, it appeared that the preference was related to an internal metabolic step. Although the protonmotive force was completely abolished by ionophores, cells retained some ability to transport pentose. In contrast, the metabolic inhibitors iodoacetate, arsenate, and fluoride had little effect on protonmotive force but caused a large decrease in intracellular ATP and xylose and arabinose uptake. These results suggested that high-affinity, ATP-dependent mechanisms were responsible for pentose transport and hexose sugars affected the utilization of xylose and arabinose.

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“…Russell and Baldwin [1] demonstrated that rumen bacteria exhibited substrate preferences and that catabolite regulatory mechanisms were used to control substrate utilization. Most work has focused on the utilization of hexose sugars by rumen organisms, but pentoses, particularly xylose and arabinose, comprise a large proportion (up to 80%) of the carbohydrate moieties found in hemicellulose ButyriL,ibrio fibrisoluens is an anaerobic bacterium which is found in the rumen, lower gastrointestinal tracts of non-ruminants, and anaerobic digesters [3,4]. It ferments a wide variety of carbohydrates and all strains are xylanolytic, with many strains metabolizing the resulting pentoses.…”

Section: Introductionmentioning

confidence: 99%

Xylose and arabinose utilization by the rumen bacterium Butyrivibrio fibrisolvens

Strobel

1993

FEMS Microbiology Letters

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The rumen bacterium Butyrivibrio fibrisolvens strain D1 co-utilized xylose and glucose in batch culture, but there was a marked preference for glucose over arabinose. When both pentoses were provided, xylose was preferred over arabinose. Strain D1 co-utilized a combination of either pentose and cellobiose, but preferred over maltose. Pentose sugars were depleted less rapidly in the presence of sucrose than controls containing only pentose. In contrast, B. fibrisolvens strain A38 exhibited a strong preference for disaccharides, including maltose, over either xylose or arabinose. Theoretical maximum growth yields for strain D1 in single-substrate continuous culture were highest for sucrose and cellobiose and the maintenance energy coefficient for arabinose was at least 3.8-fold greater than for other substrates. We suggest that B. fibrisolvens may have evolved a mechanism to utilize certain sugars before arabinose in order to avoid this high maintenance energy expenditure.

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The Anaerobic Monotrichous Butyric Acid-Producing Curved Rod-Shaped Bacteria of the Rumen

Cited by 225 publications

References 8 publications

The fructanolytic abilities of the rumen bacteriumButyrivibrio fibrisolvensstrain 3071

The fructanolytic abilities of the rumen bacteriumButyrivibrio fibrisolvensstrain 3071

Pentose transport by the ruminal bacterium Butyrivibrio fibrisolvens

Xylose and arabinose utilization by the rumen bacterium Butyrivibrio fibrisolvens

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