The Analysis of Aqueous Humor Constituents Using Capillary Zone Electrophoresis

Cowdrey, Geoffrey; Firth, Maria; Moss, R.E.; Karim, Abdul; Thompson, G M; Firth, Gary

doi:10.1006/exer.1998.0542

Cited by 5 publications

(6 citation statements)

References 4 publications

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“…The finding of protein spots derived from albumin suggests two different mechanisms for changes in the AH. Albumin is a well known constituent of aqueous humour (Krause et al 1970; Cowdrey et al 1998; Rohde et al 1998). Moreover, it is well known that albumin concentrations increase in AH in eyes with uveitis, because of breakdown of the blood–aqueous barrier (Shah et al 1992; Chiou et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of aqueous humour from patients with acute corneal rejection

Funding

Vorum

Honoré

et al. 2005

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To compare the basic proteomic composition of aqueous humour (AH) from patients with corneal rejection (patients) with AH from patients with cataract (controls). Methods: Aqueous humour was analysed for total protein concentration using Bradford's method and for protein composition using two-dimensional (2D) gel electrophoresis. Image analysis was used to detect protein spots in 2D gels that were increased by more than factor 2 in patients as compared with controls. Increased spots were identified by immunoblotting and mass spectrometry. Results: Aqueous humour from patients contained significantly higher total protein concentration than did AH from controls. A total of 31 spots were significantly increased in 2D gels from patients. The spots were derived from albumin, a1-antitrypsin, apolipoprotein J, cytokeratin type II, serin proteinase inhibitor and transthyretin. After correction of spot volumes by total protein concentrations, 10 spots derived from albumin, cytokeratin type II and a1-antitrypsin remained significantly increased. Conclusion: The proteomic composition of AH differed significantly between patients and controls. The identified proteins suggest that the changes in AH are due to at least three different mechanisms: breakdown of the aqueous-blood barrier, enzymatic degradation, and liberation of locally synthesized proteins.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of aqueous humour from patients with acute corneal rejection

Funding

Vorum

Honoré

et al. 2005

Acta Ophthalmologica Scandinavica

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“…Similar to their work in SF, Grimshaw et al determined hyaluronan in vitreous humor [149]. Using an approach adapted from their work with CSF [108], Cowdrey et al were able to profile proteins in 50-150 L samples of aqueous humor obtained from subjects undergoing surgery [150]. A borate buffer pH 9.4 with added methylcellulose revealed proteins such as albumin, transferrin and IgG, as well as organic anions such as ascorbic and lactic acid.…”

Section: Aqueous and Vitreous Humormentioning

confidence: 96%

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

Lloyd

2008

Journal of Chromatography B

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“…The human tear film is a complex three-layered structure comprising an outermost lipid layer, an aqueous layer and a mucus gel layer 22 . The lipid layer mainly consist of cholesterol esters, and ester waxes; the aqueous layer mainly contains water (99%), proteins (lysozyme, lactoferrin, lipocalin, secretory IgA), electrolytes (Na + , K + , Cl - , HCO - , Mg 2+ , Ca 2+ ), and small molecules (ascorbic acid, glucose, lactate, uric acid and sialic acid) 23 24 25 26 27 . The main component of the mucus layer is mucin, which in turn is characterized by a polymeric assembly of units forming linear polyanionic molecules.…”

Section: Resultsmentioning

confidence: 99%

Point-of-service, quantitative analysis of ascorbic acid in aqueous humor for evaluating anterior globe integrity

Gartia

Misra

et al. 2015

Sci Rep

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Limited training, high cost, and low equipment mobility leads to inaccuracies in decision making and is concerning with serious ocular injuries such as suspected ruptured globe or post-operative infections. Here, we present a novel point-of-service (POS) quantitative ascorbic acid (AA) assay with use of the OcuCheck Biosensor. The present work describes the development and clinical testing of the paper-based biosensor that measures the changes in electrical resistance of the enzyme-plated interdigitated electrodes to quantify the level of AA present in ocular fluid. We have demonstrated the proof-of-concept of the biosensor testing 16 clinical samples collected from aqueous humor of patients undergoing therapeutic anterior chamber paracentesis. Comparing with gold standard colorimetric assay for AA concentration, OcuCheck showed accuracy of >80%, sensitivity of >88% and specificity of >71%. At present, there are no FDA-approved POS tests that can directly measures AA concentration levels in ocular fluid. We envisage that the device can be realized as a handheld, battery powered instrument that will have high impact on glaucoma care and point-of-care diagnostics of penetrating ocular globe injuries.

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The Analysis of Aqueous Humor Constituents Using Capillary Zone Electrophoresis

Cited by 5 publications

References 4 publications

Proteomic analysis of aqueous humour from patients with acute corneal rejection

Proteomic analysis of aqueous humour from patients with acute corneal rejection

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

Point-of-service, quantitative analysis of ascorbic acid in aqueous humor for evaluating anterior globe integrity

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