The Analysis of Drug Resistance of the Strains of Lactic Acid Bacteria Isolated from Yoghurt Made in China and Outside of China

Abstract

To analysis and compare the drug resistance of lactic acid bacteria isolated from yoghurt made in China and outside of China. Yoghourt products were purchased from Nanchang supermarkets. The Lactic acid bacteria bacterias in yoghourt were isolated by MRS, MC and M17 medium. The isolated strains were identified by gram’s stain, colony morphology observation and biochemistry reaction test. Drug resistance analysis of the identified strains were analyzed by K-B method. 26 kinds of Lactic acid bacteria, 13 strains of cocci and 13 strains of bacillus, were isolated. All strains were identified by physiology and biochemistry test. They were Lactic acid bacteria bulgaricus, Lactic acid bacteria delbrueckii subsp. Bulgaricus, Streptococcus thermophilus. Bifidobacteria (Bacillus bifidus). Drug resistance analysis revealed that the 26 strains of Lactic acid bacteria showed multiple drug resistance to 13 kinds of antibiotics, such as kanamycin. And all of these Lactic acid bacteria were completely resistant to kanamycin, ampicillin and oxacillin. The drug resistance spectrum of China’s Lactic acid bacteria is similar to that of foreign Lactic acid bacteria, but the drug resistance of China’s Lactic acid bacteria is significantly stronger than that of foreign Lactic acid bacteria. The abuse of antibiotics is serious in dairy industry. We should strengthen the management of antibiotics, reduce the chance of super resistant bacteria, and maintain the food health of the general public.