1995
DOI: 10.1016/0014-5793(95)00097-s
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The analysis of modified peroxisome proliferator responsive elements of the peroxisomal bifunctional enzyme in transfected HepG2 cells reveals two regulatory motifs

Abstract: Key words: Peroxisome proliferator-activated receptor; Peroxisome proliferator response element; 9-cis Retinoic acid receptor alpha; Rat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase; Transcriptional activation sists of direct repeats of the sequence TGACCT, the consensus binding sequence for several members of the nuclear steroid hormone receptor superfamily [7]. PPAR binds to these PPREs through heterodimerization with the 9-cis retinoic acid receptor RXR~ [4,8]. Transcriptional activation … Show more

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Cited by 17 publications
(12 citation statements)
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“…Results from other laboratories indicate that mutations that disrupt the DR1 motifs but preserve the DR2 motif greatly diminish the responsiveness of the PPRE and the binding of PPAR/RXR (30,31). In contrast to the RevDR2 element, the 5Ј-extended sequence is not preserved in this DR2 (Table I).…”
Section: Resultsmentioning
confidence: 75%
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“…Results from other laboratories indicate that mutations that disrupt the DR1 motifs but preserve the DR2 motif greatly diminish the responsiveness of the PPRE and the binding of PPAR/RXR (30,31). In contrast to the RevDR2 element, the 5Ј-extended sequence is not preserved in this DR2 (Table I).…”
Section: Resultsmentioning
confidence: 75%
“…Naturally occurring PPREs do not generally exhibit a DR2 motif with the exception of the PPRE of the rat bifunctional enzyme that contains two DR1 motifs separated by two nucleotides (30). As reported by others, PPAR/RXR heterodimers bind to each of the DR1 motifs but not to the DR2 motif (30,31). In contrast, both binding and transactivation are evident for the RevDR2 element.…”
Section: Discussionmentioning
confidence: 72%
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“…Activation of PPARA tends to induce further expression of PPARA (Sharifpanah et al 2008) and subsequent peroxisome proliferation, including the transcription of peroxisomal b-oxidation-encoding genes such as acylCoA oxidase, multifunctional protein and thiolase (Bardot et al 1995).…”
Section: Ppara Slc16a7 and Catalasementioning
confidence: 99%
“…PPARs bind to PPRE composed of a direct repeat (DR) preferably spaced by one nucleotide (DR1) with a consensus sequence of AGGTCA-N-AGGTCA. PPARs also recognize DR2 motifs that are preferably spaced by two nucleotides [27, 28]. We first measured the expression pattern of PPARs in the HK-2 cells and found that the highest expressing isoform was PPARβ/δ followed by PPARα and PPARγ (Fig 6A).…”
Section: Resultsmentioning
confidence: 99%